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Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides
Here it is demonstrated that the yeast Saccharomyces cerevisiae can take up and assemble at least 38 overlapping single-stranded oligonucleotides and a linear double-stranded vector in one transformation event. These oligonucleotides can overlap by as few as 20 bp, and can be as long as 200 nucleoti...
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| Formato: | Texto |
| Lenguaje: | English |
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Oxford University Press
2009
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777417/ https://www.ncbi.nlm.nih.gov/pubmed/19745056 http://dx.doi.org/10.1093/nar/gkp687 |