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Evidence of the crucial role of the linker domain on the catalytic activity of human topoisomerase I by experimental and simulative characterization of the Lys681Ala mutant

The functional and structural-dynamical properties of the Lys681Ala mutation in the human topoisomerase IB linker domain have been investigated by catalytic assays and molecular dynamics simulation. The mutant is characterized by a comparable cleavage and a strongly reduced religation rate when comp...

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Detalles Bibliográficos
Autores principales:	Fiorani, Paola, Tesauro, Cinzia, Mancini, Giordano, Chillemi, Giovanni, D'A;nnessa, Ilda, Graziani, Grazia, Tentori, Lucio, Muzi, Alessia, Desideri, Alessandro
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2009
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777420/ https://www.ncbi.nlm.nih.gov/pubmed/19767617 http://dx.doi.org/10.1093/nar/gkp669

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