Time-resolved fluorescence studies of nucleotide flipping by restriction enzymes

Restriction enzymes Ecl18kI, PspGI and EcoRII-C, specific for interrupted 5-bp target sequences, flip the central base pair of these sequences into their protein pockets to facilitate sequence recognition and adjust the DNA cleavage pattern. We have used time-resolved fluorescence spectroscopy of 2-...

Descripción completa

Detalles Bibliográficos
Autores principales: Neely, Robert K., Tamulaitis, Gintautas, Chen, Kai, Kubala, Marta, Siksnys, Virginijus, Jones, Anita C.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2009
Materias:
Nucleic Acid Enzymes
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777440/
https://www.ncbi.nlm.nih.gov/pubmed/19740769
http://dx.doi.org/10.1093/nar/gkp688

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777440/
https://www.ncbi.nlm.nih.gov/pubmed/19740769
http://dx.doi.org/10.1093/nar/gkp688

Ejemplares similares