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Time-resolved fluorescence studies of nucleotide flipping by restriction enzymes
Restriction enzymes Ecl18kI, PspGI and EcoRII-C, specific for interrupted 5-bp target sequences, flip the central base pair of these sequences into their protein pockets to facilitate sequence recognition and adjust the DNA cleavage pattern. We have used time-resolved fluorescence spectroscopy of 2-...
Autores principales: | Neely, Robert K., Tamulaitis, Gintautas, Chen, Kai, Kubala, Marta, Siksnys, Virginijus, Jones, Anita C. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777440/ https://www.ncbi.nlm.nih.gov/pubmed/19740769 http://dx.doi.org/10.1093/nar/gkp688 |
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