Cargando…
MeCP2/H3meK9 are involved in IL-6 gene silencing in pancreatic adenocarcinoma cell lines
The aim of the present study was to analyse the molecular mechanisms involved in the Interleukin-6 (IL-6) silencing in pancreatic adenocarcinoma cell lines. Our results demonstrate that TNF-α, a major IL-6 inducer, is able to induce IL-6 only in three out of six cell lines examined. 5-aza-2′-deoxycy...
Autores principales: | , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777443/ https://www.ncbi.nlm.nih.gov/pubmed/19745053 http://dx.doi.org/10.1093/nar/gkp723 |
_version_ | 1782174192029925376 |
---|---|
author | Dandrea, Mario Donadelli, Massimo Costanzo, Chiara Scarpa, Aldo Palmieri, Marta |
author_facet | Dandrea, Mario Donadelli, Massimo Costanzo, Chiara Scarpa, Aldo Palmieri, Marta |
author_sort | Dandrea, Mario |
collection | PubMed |
description | The aim of the present study was to analyse the molecular mechanisms involved in the Interleukin-6 (IL-6) silencing in pancreatic adenocarcinoma cell lines. Our results demonstrate that TNF-α, a major IL-6 inducer, is able to induce IL-6 only in three out of six cell lines examined. 5-aza-2′-deoxycytidine (DAC), but not trichostatin A (TSA), activates the expression of IL-6 in all cell lines, indicating that DNA methylation, but not histone deacetylation, plays an essential role in IL-6 silencing. Indeed, the IL-6 upstream region shows a methylation status that correlates with IL-6 expression and binds MeCP2 and H3meK9 only in the non-expressing cell lines. Our results suggest that critical methylations located from positions –666 to –426 relative to the transcription start site of IL-6 may act as binding sites for MeCP2. |
format | Text |
id | pubmed-2777443 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27774432009-11-16 MeCP2/H3meK9 are involved in IL-6 gene silencing in pancreatic adenocarcinoma cell lines Dandrea, Mario Donadelli, Massimo Costanzo, Chiara Scarpa, Aldo Palmieri, Marta Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics The aim of the present study was to analyse the molecular mechanisms involved in the Interleukin-6 (IL-6) silencing in pancreatic adenocarcinoma cell lines. Our results demonstrate that TNF-α, a major IL-6 inducer, is able to induce IL-6 only in three out of six cell lines examined. 5-aza-2′-deoxycytidine (DAC), but not trichostatin A (TSA), activates the expression of IL-6 in all cell lines, indicating that DNA methylation, but not histone deacetylation, plays an essential role in IL-6 silencing. Indeed, the IL-6 upstream region shows a methylation status that correlates with IL-6 expression and binds MeCP2 and H3meK9 only in the non-expressing cell lines. Our results suggest that critical methylations located from positions –666 to –426 relative to the transcription start site of IL-6 may act as binding sites for MeCP2. Oxford University Press 2009-11 2009-09-10 /pmc/articles/PMC2777443/ /pubmed/19745053 http://dx.doi.org/10.1093/nar/gkp723 Text en © The Author(s) 2009. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Gene Regulation, Chromatin and Epigenetics Dandrea, Mario Donadelli, Massimo Costanzo, Chiara Scarpa, Aldo Palmieri, Marta MeCP2/H3meK9 are involved in IL-6 gene silencing in pancreatic adenocarcinoma cell lines |
title | MeCP2/H3meK9 are involved in IL-6 gene silencing in pancreatic adenocarcinoma cell lines |
title_full | MeCP2/H3meK9 are involved in IL-6 gene silencing in pancreatic adenocarcinoma cell lines |
title_fullStr | MeCP2/H3meK9 are involved in IL-6 gene silencing in pancreatic adenocarcinoma cell lines |
title_full_unstemmed | MeCP2/H3meK9 are involved in IL-6 gene silencing in pancreatic adenocarcinoma cell lines |
title_short | MeCP2/H3meK9 are involved in IL-6 gene silencing in pancreatic adenocarcinoma cell lines |
title_sort | mecp2/h3mek9 are involved in il-6 gene silencing in pancreatic adenocarcinoma cell lines |
topic | Gene Regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777443/ https://www.ncbi.nlm.nih.gov/pubmed/19745053 http://dx.doi.org/10.1093/nar/gkp723 |
work_keys_str_mv | AT dandreamario mecp2h3mek9areinvolvedinil6genesilencinginpancreaticadenocarcinomacelllines AT donadellimassimo mecp2h3mek9areinvolvedinil6genesilencinginpancreaticadenocarcinomacelllines AT costanzochiara mecp2h3mek9areinvolvedinil6genesilencinginpancreaticadenocarcinomacelllines AT scarpaaldo mecp2h3mek9areinvolvedinil6genesilencinginpancreaticadenocarcinomacelllines AT palmierimarta mecp2h3mek9areinvolvedinil6genesilencinginpancreaticadenocarcinomacelllines |