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Genome-wide analysis of a G-quadruplex-specific single-chain antibody that regulates gene expression
G-quadruplex nucleic acids have been proposed to play a role in a number of fundamental biological processes that include transcription and translation. We have developed a single-chain antibody that is selective for G-quadruplex DNA over double-stranded DNA, and here show that when it is expressed...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777450/ https://www.ncbi.nlm.nih.gov/pubmed/19745055 http://dx.doi.org/10.1093/nar/gkp740 |
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author | Fernando, Himesh Sewitz, Sven Darot, Jeremy Tavaré, Simon Huppert, Julian Leon Balasubramanian, Shankar |
author_facet | Fernando, Himesh Sewitz, Sven Darot, Jeremy Tavaré, Simon Huppert, Julian Leon Balasubramanian, Shankar |
author_sort | Fernando, Himesh |
collection | PubMed |
description | G-quadruplex nucleic acids have been proposed to play a role in a number of fundamental biological processes that include transcription and translation. We have developed a single-chain antibody that is selective for G-quadruplex DNA over double-stranded DNA, and here show that when it is expressed in human cells, it significantly affects the expression of a wide variety of genes, in a manner that correlates with the presence of predicted G-quadruplexes. We observe cases where gene expression is increased or decreased, and that there are apparent interactions with G-quadruplex motifs at the beginning and end of the genes, and on either strand. The outcomes of this genome-wide study demonstrate that G-quadruplex recognition by the antibody has physiological consequences, and provides insights into some of the complexity associated with G-quadruplex-based regulation. |
format | Text |
id | pubmed-2777450 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27774502009-11-16 Genome-wide analysis of a G-quadruplex-specific single-chain antibody that regulates gene expression Fernando, Himesh Sewitz, Sven Darot, Jeremy Tavaré, Simon Huppert, Julian Leon Balasubramanian, Shankar Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics G-quadruplex nucleic acids have been proposed to play a role in a number of fundamental biological processes that include transcription and translation. We have developed a single-chain antibody that is selective for G-quadruplex DNA over double-stranded DNA, and here show that when it is expressed in human cells, it significantly affects the expression of a wide variety of genes, in a manner that correlates with the presence of predicted G-quadruplexes. We observe cases where gene expression is increased or decreased, and that there are apparent interactions with G-quadruplex motifs at the beginning and end of the genes, and on either strand. The outcomes of this genome-wide study demonstrate that G-quadruplex recognition by the antibody has physiological consequences, and provides insights into some of the complexity associated with G-quadruplex-based regulation. Oxford University Press 2009-11 2009-09-10 /pmc/articles/PMC2777450/ /pubmed/19745055 http://dx.doi.org/10.1093/nar/gkp740 Text en © The Author(s) 2009. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Gene Regulation, Chromatin and Epigenetics Fernando, Himesh Sewitz, Sven Darot, Jeremy Tavaré, Simon Huppert, Julian Leon Balasubramanian, Shankar Genome-wide analysis of a G-quadruplex-specific single-chain antibody that regulates gene expression |
title | Genome-wide analysis of a G-quadruplex-specific single-chain antibody that regulates gene expression |
title_full | Genome-wide analysis of a G-quadruplex-specific single-chain antibody that regulates gene expression |
title_fullStr | Genome-wide analysis of a G-quadruplex-specific single-chain antibody that regulates gene expression |
title_full_unstemmed | Genome-wide analysis of a G-quadruplex-specific single-chain antibody that regulates gene expression |
title_short | Genome-wide analysis of a G-quadruplex-specific single-chain antibody that regulates gene expression |
title_sort | genome-wide analysis of a g-quadruplex-specific single-chain antibody that regulates gene expression |
topic | Gene Regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777450/ https://www.ncbi.nlm.nih.gov/pubmed/19745055 http://dx.doi.org/10.1093/nar/gkp740 |
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