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Translocation of double stranded DNA through membrane adapted phi29 motor protein nanopore
Biological pores have been used to study the transport of DNA and other molecules but most pores have channels that allow only the movement of small molecules and single-stranded DNA and RNA. The bacteriophage phi29 DNA-packaging motor, which allows double-stranded DNA to enter and exit during a vir...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777743/ https://www.ncbi.nlm.nih.gov/pubmed/19893523 http://dx.doi.org/10.1038/nnano.2009.259 |
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author | Wendell, David Jing, Peng Geng, Jia Subramaniam, Varuni Lee, Tae Jin Montemagno, Carlo Guo, Peixuan |
author_facet | Wendell, David Jing, Peng Geng, Jia Subramaniam, Varuni Lee, Tae Jin Montemagno, Carlo Guo, Peixuan |
author_sort | Wendell, David |
collection | PubMed |
description | Biological pores have been used to study the transport of DNA and other molecules but most pores have channels that allow only the movement of small molecules and single-stranded DNA and RNA. The bacteriophage phi29 DNA-packaging motor, which allows double-stranded DNA to enter and exit during a viral infection, contains a connector protein that has a 3.6 – 6.0 nm wide channel. Here we show that a modified version of the connector protein, when reconstituted into liposomes and inserted into planar lipid bilayers, can act as conductive channels to allow the translocation of double-stranded DNA. Single-channel conductance assays and quantitative PCR confirmed the translocation through the pore. The measured conductance of a single connector channel was 4.8 nS in 1 M KCl. This engineered and membrane-adapted phage connector is expected to have interesting applications in nanotechnology and nanomedicine, such as MEMS sensing, microreactors, gene delivery, drug loading, and DNA sequencing. |
format | Text |
id | pubmed-2777743 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
record_format | MEDLINE/PubMed |
spelling | pubmed-27777432010-05-01 Translocation of double stranded DNA through membrane adapted phi29 motor protein nanopore Wendell, David Jing, Peng Geng, Jia Subramaniam, Varuni Lee, Tae Jin Montemagno, Carlo Guo, Peixuan Nat Nanotechnol Article Biological pores have been used to study the transport of DNA and other molecules but most pores have channels that allow only the movement of small molecules and single-stranded DNA and RNA. The bacteriophage phi29 DNA-packaging motor, which allows double-stranded DNA to enter and exit during a viral infection, contains a connector protein that has a 3.6 – 6.0 nm wide channel. Here we show that a modified version of the connector protein, when reconstituted into liposomes and inserted into planar lipid bilayers, can act as conductive channels to allow the translocation of double-stranded DNA. Single-channel conductance assays and quantitative PCR confirmed the translocation through the pore. The measured conductance of a single connector channel was 4.8 nS in 1 M KCl. This engineered and membrane-adapted phage connector is expected to have interesting applications in nanotechnology and nanomedicine, such as MEMS sensing, microreactors, gene delivery, drug loading, and DNA sequencing. 2009-09-27 2009-11 /pmc/articles/PMC2777743/ /pubmed/19893523 http://dx.doi.org/10.1038/nnano.2009.259 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Wendell, David Jing, Peng Geng, Jia Subramaniam, Varuni Lee, Tae Jin Montemagno, Carlo Guo, Peixuan Translocation of double stranded DNA through membrane adapted phi29 motor protein nanopore |
title | Translocation of double stranded DNA through membrane adapted phi29 motor protein nanopore |
title_full | Translocation of double stranded DNA through membrane adapted phi29 motor protein nanopore |
title_fullStr | Translocation of double stranded DNA through membrane adapted phi29 motor protein nanopore |
title_full_unstemmed | Translocation of double stranded DNA through membrane adapted phi29 motor protein nanopore |
title_short | Translocation of double stranded DNA through membrane adapted phi29 motor protein nanopore |
title_sort | translocation of double stranded dna through membrane adapted phi29 motor protein nanopore |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2777743/ https://www.ncbi.nlm.nih.gov/pubmed/19893523 http://dx.doi.org/10.1038/nnano.2009.259 |
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