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Characterization of Acute Lymphoblastic Leukemia Subtypes in Moroccan Children
We present the incidence and the immunologic characteristics of acute lymphoblastic leukemia (ALL) subsets in Moroccan children. We studied 279 unselected patients below the age of 18 years with newly diagnosed ALL. Cases were classified according to immunophenotype: 216 (77.42%) precursor B-cell ph...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2778177/ https://www.ncbi.nlm.nih.gov/pubmed/20041009 http://dx.doi.org/10.1155/2009/674801 |
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author | Bachir, Fatima Bennani, Sanae Lahjouji, Ali Cherkaoui, Siham Harif, M'hamed Khattab, Mohamed Nassereddine, Ilham Zafad, Saadia El Aouad, Rajae |
author_facet | Bachir, Fatima Bennani, Sanae Lahjouji, Ali Cherkaoui, Siham Harif, M'hamed Khattab, Mohamed Nassereddine, Ilham Zafad, Saadia El Aouad, Rajae |
author_sort | Bachir, Fatima |
collection | PubMed |
description | We present the incidence and the immunologic characteristics of acute lymphoblastic leukemia (ALL) subsets in Moroccan children. We studied 279 unselected patients below the age of 18 years with newly diagnosed ALL. Cases were classified according to immunophenotype: 216 (77.42%) precursor B-cell phenotype (pB-cell), mature B-cell in 4 (1.43%), and T-cell in 59 (21.15%) cases. The subclassification using the CD10 antibody revealed 197 cases pB-ALL CD10+ (91.2%) and 9 cases T-ALL CD10+ (19.2%). The age distribution showed a peak in incidence between 3 and 5 years among the pB-cell ALLs subtype. There was a significantly higher frequency of males in the T-ALL subset (M/F ratio: 2.93 : 1) and more females in the T-ALL CD10+ subset when compared with the T-ALL CD10– subset. All tested pB-cell-lineage ALLs expressed CD19, CD79a, and surface CD22, terminal deoxynucleotidyl transferase (TdT) was detectable in 89.9% of cases, and cells in 74.1% of cases express CD34. All tested T-lineage ALL cells have surface CD7 and cytoplasmic CD3 (cCD3) antigens, CD5 was found in 98.2% cases, and 70.5% express TdT. CD1a, surface CD3 (sCD3), and CD4 are detected in more than 80% of cases; this frequency is higher than the 45% generally observed. Myeloid antigens occur more frequently and were expressed in 124 (57.4%) of pB-cell-ALL cases and 20 (33.9%) of T-cell ALL cases. Our results show that the distribution of ALLs in Moroccan children is similar with the general distribution pattern in developed countries except for the high frequency of T-ALL phenotype. The phenotypic profiles of our patients are close to those reported in literature for B-lineage ALLs; for the T-cell ALL subgroup, the blast cells express more CD1a, surface CD3, and CD4 while expressing less TdT. The high frequency of CD1a expression resulted in an excess of the common thymocyte subtype. |
format | Text |
id | pubmed-2778177 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-27781772009-12-29 Characterization of Acute Lymphoblastic Leukemia Subtypes in Moroccan Children Bachir, Fatima Bennani, Sanae Lahjouji, Ali Cherkaoui, Siham Harif, M'hamed Khattab, Mohamed Nassereddine, Ilham Zafad, Saadia El Aouad, Rajae Int J Pediatr Clinical Study We present the incidence and the immunologic characteristics of acute lymphoblastic leukemia (ALL) subsets in Moroccan children. We studied 279 unselected patients below the age of 18 years with newly diagnosed ALL. Cases were classified according to immunophenotype: 216 (77.42%) precursor B-cell phenotype (pB-cell), mature B-cell in 4 (1.43%), and T-cell in 59 (21.15%) cases. The subclassification using the CD10 antibody revealed 197 cases pB-ALL CD10+ (91.2%) and 9 cases T-ALL CD10+ (19.2%). The age distribution showed a peak in incidence between 3 and 5 years among the pB-cell ALLs subtype. There was a significantly higher frequency of males in the T-ALL subset (M/F ratio: 2.93 : 1) and more females in the T-ALL CD10+ subset when compared with the T-ALL CD10– subset. All tested pB-cell-lineage ALLs expressed CD19, CD79a, and surface CD22, terminal deoxynucleotidyl transferase (TdT) was detectable in 89.9% of cases, and cells in 74.1% of cases express CD34. All tested T-lineage ALL cells have surface CD7 and cytoplasmic CD3 (cCD3) antigens, CD5 was found in 98.2% cases, and 70.5% express TdT. CD1a, surface CD3 (sCD3), and CD4 are detected in more than 80% of cases; this frequency is higher than the 45% generally observed. Myeloid antigens occur more frequently and were expressed in 124 (57.4%) of pB-cell-ALL cases and 20 (33.9%) of T-cell ALL cases. Our results show that the distribution of ALLs in Moroccan children is similar with the general distribution pattern in developed countries except for the high frequency of T-ALL phenotype. The phenotypic profiles of our patients are close to those reported in literature for B-lineage ALLs; for the T-cell ALL subgroup, the blast cells express more CD1a, surface CD3, and CD4 while expressing less TdT. The high frequency of CD1a expression resulted in an excess of the common thymocyte subtype. Hindawi Publishing Corporation 2009 2009-07-19 /pmc/articles/PMC2778177/ /pubmed/20041009 http://dx.doi.org/10.1155/2009/674801 Text en Copyright © 2009 Fatima Bachir et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Clinical Study Bachir, Fatima Bennani, Sanae Lahjouji, Ali Cherkaoui, Siham Harif, M'hamed Khattab, Mohamed Nassereddine, Ilham Zafad, Saadia El Aouad, Rajae Characterization of Acute Lymphoblastic Leukemia Subtypes in Moroccan Children |
title | Characterization of Acute Lymphoblastic Leukemia Subtypes in Moroccan Children |
title_full | Characterization of Acute Lymphoblastic Leukemia Subtypes in Moroccan Children |
title_fullStr | Characterization of Acute Lymphoblastic Leukemia Subtypes in Moroccan Children |
title_full_unstemmed | Characterization of Acute Lymphoblastic Leukemia Subtypes in Moroccan Children |
title_short | Characterization of Acute Lymphoblastic Leukemia Subtypes in Moroccan Children |
title_sort | characterization of acute lymphoblastic leukemia subtypes in moroccan children |
topic | Clinical Study |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2778177/ https://www.ncbi.nlm.nih.gov/pubmed/20041009 http://dx.doi.org/10.1155/2009/674801 |
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