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A dual function for Pex3p in peroxisome formation and inheritance

Saccharomyces cerevisiae Pex3p has been shown to act at the ER during de novo peroxisome formation. However, its steady state is at the peroxisomal membrane, where its role is debated. Here we show that Pex3p has a dual function: one in peroxisome formation and one in peroxisome segregation. We show...

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Detalles Bibliográficos
Autores principales: Munck, Joanne M., Motley, Alison M., Nuttall, James M., Hettema, Ewald H.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779223/
https://www.ncbi.nlm.nih.gov/pubmed/19948495
http://dx.doi.org/10.1083/jcb.200906161
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author Munck, Joanne M.
Motley, Alison M.
Nuttall, James M.
Hettema, Ewald H.
author_facet Munck, Joanne M.
Motley, Alison M.
Nuttall, James M.
Hettema, Ewald H.
author_sort Munck, Joanne M.
collection PubMed
description Saccharomyces cerevisiae Pex3p has been shown to act at the ER during de novo peroxisome formation. However, its steady state is at the peroxisomal membrane, where its role is debated. Here we show that Pex3p has a dual function: one in peroxisome formation and one in peroxisome segregation. We show that the peroxisome retention factor Inp1p interacts physically with Pex3p in vitro and in vivo, and split-GFP analysis shows that the site of interaction is the peroxisomal membrane. Furthermore, we have generated PEX3 alleles that support peroxisome formation but fail to support recruitment of Inp1p to peroxisomes, and as a consequence are affected in peroxisome segregation. We conclude that Pex3p functions as an anchor for Inp1p at the peroxisomal membrane, and that this function is independent of its role at the ER in peroxisome biogenesis.
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spelling pubmed-27792232010-05-16 A dual function for Pex3p in peroxisome formation and inheritance Munck, Joanne M. Motley, Alison M. Nuttall, James M. Hettema, Ewald H. J Cell Biol Research Articles Saccharomyces cerevisiae Pex3p has been shown to act at the ER during de novo peroxisome formation. However, its steady state is at the peroxisomal membrane, where its role is debated. Here we show that Pex3p has a dual function: one in peroxisome formation and one in peroxisome segregation. We show that the peroxisome retention factor Inp1p interacts physically with Pex3p in vitro and in vivo, and split-GFP analysis shows that the site of interaction is the peroxisomal membrane. Furthermore, we have generated PEX3 alleles that support peroxisome formation but fail to support recruitment of Inp1p to peroxisomes, and as a consequence are affected in peroxisome segregation. We conclude that Pex3p functions as an anchor for Inp1p at the peroxisomal membrane, and that this function is independent of its role at the ER in peroxisome biogenesis. The Rockefeller University Press 2009-11-16 /pmc/articles/PMC2779223/ /pubmed/19948495 http://dx.doi.org/10.1083/jcb.200906161 Text en © 2009 Munck et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Munck, Joanne M.
Motley, Alison M.
Nuttall, James M.
Hettema, Ewald H.
A dual function for Pex3p in peroxisome formation and inheritance
title A dual function for Pex3p in peroxisome formation and inheritance
title_full A dual function for Pex3p in peroxisome formation and inheritance
title_fullStr A dual function for Pex3p in peroxisome formation and inheritance
title_full_unstemmed A dual function for Pex3p in peroxisome formation and inheritance
title_short A dual function for Pex3p in peroxisome formation and inheritance
title_sort dual function for pex3p in peroxisome formation and inheritance
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779223/
https://www.ncbi.nlm.nih.gov/pubmed/19948495
http://dx.doi.org/10.1083/jcb.200906161
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