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A dual function for Pex3p in peroxisome formation and inheritance
Saccharomyces cerevisiae Pex3p has been shown to act at the ER during de novo peroxisome formation. However, its steady state is at the peroxisomal membrane, where its role is debated. Here we show that Pex3p has a dual function: one in peroxisome formation and one in peroxisome segregation. We show...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779223/ https://www.ncbi.nlm.nih.gov/pubmed/19948495 http://dx.doi.org/10.1083/jcb.200906161 |
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author | Munck, Joanne M. Motley, Alison M. Nuttall, James M. Hettema, Ewald H. |
author_facet | Munck, Joanne M. Motley, Alison M. Nuttall, James M. Hettema, Ewald H. |
author_sort | Munck, Joanne M. |
collection | PubMed |
description | Saccharomyces cerevisiae Pex3p has been shown to act at the ER during de novo peroxisome formation. However, its steady state is at the peroxisomal membrane, where its role is debated. Here we show that Pex3p has a dual function: one in peroxisome formation and one in peroxisome segregation. We show that the peroxisome retention factor Inp1p interacts physically with Pex3p in vitro and in vivo, and split-GFP analysis shows that the site of interaction is the peroxisomal membrane. Furthermore, we have generated PEX3 alleles that support peroxisome formation but fail to support recruitment of Inp1p to peroxisomes, and as a consequence are affected in peroxisome segregation. We conclude that Pex3p functions as an anchor for Inp1p at the peroxisomal membrane, and that this function is independent of its role at the ER in peroxisome biogenesis. |
format | Text |
id | pubmed-2779223 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27792232010-05-16 A dual function for Pex3p in peroxisome formation and inheritance Munck, Joanne M. Motley, Alison M. Nuttall, James M. Hettema, Ewald H. J Cell Biol Research Articles Saccharomyces cerevisiae Pex3p has been shown to act at the ER during de novo peroxisome formation. However, its steady state is at the peroxisomal membrane, where its role is debated. Here we show that Pex3p has a dual function: one in peroxisome formation and one in peroxisome segregation. We show that the peroxisome retention factor Inp1p interacts physically with Pex3p in vitro and in vivo, and split-GFP analysis shows that the site of interaction is the peroxisomal membrane. Furthermore, we have generated PEX3 alleles that support peroxisome formation but fail to support recruitment of Inp1p to peroxisomes, and as a consequence are affected in peroxisome segregation. We conclude that Pex3p functions as an anchor for Inp1p at the peroxisomal membrane, and that this function is independent of its role at the ER in peroxisome biogenesis. The Rockefeller University Press 2009-11-16 /pmc/articles/PMC2779223/ /pubmed/19948495 http://dx.doi.org/10.1083/jcb.200906161 Text en © 2009 Munck et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Munck, Joanne M. Motley, Alison M. Nuttall, James M. Hettema, Ewald H. A dual function for Pex3p in peroxisome formation and inheritance |
title | A dual function for Pex3p in peroxisome formation and inheritance |
title_full | A dual function for Pex3p in peroxisome formation and inheritance |
title_fullStr | A dual function for Pex3p in peroxisome formation and inheritance |
title_full_unstemmed | A dual function for Pex3p in peroxisome formation and inheritance |
title_short | A dual function for Pex3p in peroxisome formation and inheritance |
title_sort | dual function for pex3p in peroxisome formation and inheritance |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779223/ https://www.ncbi.nlm.nih.gov/pubmed/19948495 http://dx.doi.org/10.1083/jcb.200906161 |
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