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Quantitative analysis of chromatin compaction in living cells using FLIM–FRET

We present a quantitative Förster resonance energy transfer (FRET)–based assay using multiphoton fluorescence lifetime imaging microscopy (FLIM) to measure chromatin compaction at the scale of nucleosomal arrays in live cells. The assay uses a human cell line coexpressing histone H2B tagged to eithe...

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Detalles Bibliográficos
Autores principales:	Llères, David, James, John, Swift, Sam, Norman, David G., Lamond, Angus I.
Formato:	Texto
Lenguaje:	English
Publicado:	The Rockefeller University Press 2009
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779238/ https://www.ncbi.nlm.nih.gov/pubmed/19948497 http://dx.doi.org/10.1083/jcb.200907029

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