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Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1

Nodal and Notch signaling pathways play essential roles in vertebrate development. Through a yeast two-hybrid screening, we identified Notch3 as a candidate binding partner of the Nodal coreceptor Cripto-1. Coimmunoprecipitation analysis confirmed the binding of Cripto-1 with all four mammalian Notc...

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Autores principales: Watanabe, Kazuhide, Nagaoka, Tadahiro, Lee, Joseph M., Bianco, Caterina, Gonzales, Monica, Castro, Nadia P., Rangel, Maria Cristina, Sakamoto, Kei, Sun, Youping, Callahan, Robert, Salomon, David S.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779239/
https://www.ncbi.nlm.nih.gov/pubmed/19948478
http://dx.doi.org/10.1083/jcb.200905105
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author Watanabe, Kazuhide
Nagaoka, Tadahiro
Lee, Joseph M.
Bianco, Caterina
Gonzales, Monica
Castro, Nadia P.
Rangel, Maria Cristina
Sakamoto, Kei
Sun, Youping
Callahan, Robert
Salomon, David S.
author_facet Watanabe, Kazuhide
Nagaoka, Tadahiro
Lee, Joseph M.
Bianco, Caterina
Gonzales, Monica
Castro, Nadia P.
Rangel, Maria Cristina
Sakamoto, Kei
Sun, Youping
Callahan, Robert
Salomon, David S.
author_sort Watanabe, Kazuhide
collection PubMed
description Nodal and Notch signaling pathways play essential roles in vertebrate development. Through a yeast two-hybrid screening, we identified Notch3 as a candidate binding partner of the Nodal coreceptor Cripto-1. Coimmunoprecipitation analysis confirmed the binding of Cripto-1 with all four mammalian Notch receptors. Deletion analyses revealed that the binding of Cripto-1 and Notch1 is mediated by the Cripto-1/FRL-1/Cryptic domain of Cripto-1 and the C-terminal region of epidermal growth factor–like repeats of Notch1. Binding of Cripto-1 to Notch1 occurred mainly in the endoplasmic reticulum–Golgi network. Cripto-1 expression resulted in the recruitment of Notch1 protein into lipid raft microdomains and enhancement of the furin-like protein convertase-mediated proteolytic maturation of Notch1 (S1 cleavage). Enhanced S1 cleavage resulted in the sensitization to ligand-induced activation of Notch signaling. In addition, knockdown of Cripto-1 expression in human and mouse embryonal carcinoma cells desensitized the ligand-induced Notch signaling activation. These results suggest a novel role of Cripto-1 in facilitating the posttranslational maturation of Notch receptors.
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spelling pubmed-27792392010-05-02 Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1 Watanabe, Kazuhide Nagaoka, Tadahiro Lee, Joseph M. Bianco, Caterina Gonzales, Monica Castro, Nadia P. Rangel, Maria Cristina Sakamoto, Kei Sun, Youping Callahan, Robert Salomon, David S. J Cell Biol Research Articles Nodal and Notch signaling pathways play essential roles in vertebrate development. Through a yeast two-hybrid screening, we identified Notch3 as a candidate binding partner of the Nodal coreceptor Cripto-1. Coimmunoprecipitation analysis confirmed the binding of Cripto-1 with all four mammalian Notch receptors. Deletion analyses revealed that the binding of Cripto-1 and Notch1 is mediated by the Cripto-1/FRL-1/Cryptic domain of Cripto-1 and the C-terminal region of epidermal growth factor–like repeats of Notch1. Binding of Cripto-1 to Notch1 occurred mainly in the endoplasmic reticulum–Golgi network. Cripto-1 expression resulted in the recruitment of Notch1 protein into lipid raft microdomains and enhancement of the furin-like protein convertase-mediated proteolytic maturation of Notch1 (S1 cleavage). Enhanced S1 cleavage resulted in the sensitization to ligand-induced activation of Notch signaling. In addition, knockdown of Cripto-1 expression in human and mouse embryonal carcinoma cells desensitized the ligand-induced Notch signaling activation. These results suggest a novel role of Cripto-1 in facilitating the posttranslational maturation of Notch receptors. The Rockefeller University Press 2009-11-02 /pmc/articles/PMC2779239/ /pubmed/19948478 http://dx.doi.org/10.1083/jcb.200905105 Text en © 2009 The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Watanabe, Kazuhide
Nagaoka, Tadahiro
Lee, Joseph M.
Bianco, Caterina
Gonzales, Monica
Castro, Nadia P.
Rangel, Maria Cristina
Sakamoto, Kei
Sun, Youping
Callahan, Robert
Salomon, David S.
Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1
title Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1
title_full Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1
title_fullStr Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1
title_full_unstemmed Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1
title_short Enhancement of Notch receptor maturation and signaling sensitivity by Cripto-1
title_sort enhancement of notch receptor maturation and signaling sensitivity by cripto-1
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779239/
https://www.ncbi.nlm.nih.gov/pubmed/19948478
http://dx.doi.org/10.1083/jcb.200905105
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