Cargando…
Heterogeneity in the physiological states and pharmacological responses of differentiating 3T3-L1 preadipocytes
Increases in key components of adipogenesis and lipolysis pathways correlate at the population-averaged level during adipogenesis. However, differentiating preadipocytes are highly heterogeneous in cellular and lipid droplet (LD) morphologies, and the degree to which individual cells follow populati...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779244/ https://www.ncbi.nlm.nih.gov/pubmed/19948481 http://dx.doi.org/10.1083/jcb.200904140 |
_version_ | 1782174354744803328 |
---|---|
author | Loo, Lit-Hsin Lin, Hai-Jui Singh, Dinesh K. Lyons, Kathleen M. Altschuler, Steven J. Wu, Lani F. |
author_facet | Loo, Lit-Hsin Lin, Hai-Jui Singh, Dinesh K. Lyons, Kathleen M. Altschuler, Steven J. Wu, Lani F. |
author_sort | Loo, Lit-Hsin |
collection | PubMed |
description | Increases in key components of adipogenesis and lipolysis pathways correlate at the population-averaged level during adipogenesis. However, differentiating preadipocytes are highly heterogeneous in cellular and lipid droplet (LD) morphologies, and the degree to which individual cells follow population-averaged trends is unclear. In this study, we analyze the molecular heterogeneity of differentiating 3T3-L1 preadipocytes using immunofluorescence microscopy. Unexpectedly, we only observe a small percentage of cells with high simultaneous expression of markers for adipogenesis (peroxisome proliferator-activated receptor γ [PPARγ], CCAAT/enhancer-binding protein α, and adiponectin) and lipid accumulation (hormone-sensitive lipase, perilipin A, and LDs). Instead, we identify subpopulations of cells with negatively correlated expressions of these readouts. Acute perturbation of adipocyte differentiation with PPARγ agonists, forskolin, and fatty acids induced subpopulation-specific effects, including redistribution of the percentage of cells in observed subpopulations and differential expression levels of PPARγ. Collectively, our results suggested that heterogeneity observed during 3T3-L1 adipogenesis reflects a dynamic mixture of subpopulations with distinct physiological states. |
format | Text |
id | pubmed-2779244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27792442010-05-02 Heterogeneity in the physiological states and pharmacological responses of differentiating 3T3-L1 preadipocytes Loo, Lit-Hsin Lin, Hai-Jui Singh, Dinesh K. Lyons, Kathleen M. Altschuler, Steven J. Wu, Lani F. J Cell Biol Research Articles Increases in key components of adipogenesis and lipolysis pathways correlate at the population-averaged level during adipogenesis. However, differentiating preadipocytes are highly heterogeneous in cellular and lipid droplet (LD) morphologies, and the degree to which individual cells follow population-averaged trends is unclear. In this study, we analyze the molecular heterogeneity of differentiating 3T3-L1 preadipocytes using immunofluorescence microscopy. Unexpectedly, we only observe a small percentage of cells with high simultaneous expression of markers for adipogenesis (peroxisome proliferator-activated receptor γ [PPARγ], CCAAT/enhancer-binding protein α, and adiponectin) and lipid accumulation (hormone-sensitive lipase, perilipin A, and LDs). Instead, we identify subpopulations of cells with negatively correlated expressions of these readouts. Acute perturbation of adipocyte differentiation with PPARγ agonists, forskolin, and fatty acids induced subpopulation-specific effects, including redistribution of the percentage of cells in observed subpopulations and differential expression levels of PPARγ. Collectively, our results suggested that heterogeneity observed during 3T3-L1 adipogenesis reflects a dynamic mixture of subpopulations with distinct physiological states. The Rockefeller University Press 2009-11-02 /pmc/articles/PMC2779244/ /pubmed/19948481 http://dx.doi.org/10.1083/jcb.200904140 Text en © 2009 Loo et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/). |
spellingShingle | Research Articles Loo, Lit-Hsin Lin, Hai-Jui Singh, Dinesh K. Lyons, Kathleen M. Altschuler, Steven J. Wu, Lani F. Heterogeneity in the physiological states and pharmacological responses of differentiating 3T3-L1 preadipocytes |
title | Heterogeneity in the physiological states and pharmacological responses of differentiating 3T3-L1 preadipocytes |
title_full | Heterogeneity in the physiological states and pharmacological responses of differentiating 3T3-L1 preadipocytes |
title_fullStr | Heterogeneity in the physiological states and pharmacological responses of differentiating 3T3-L1 preadipocytes |
title_full_unstemmed | Heterogeneity in the physiological states and pharmacological responses of differentiating 3T3-L1 preadipocytes |
title_short | Heterogeneity in the physiological states and pharmacological responses of differentiating 3T3-L1 preadipocytes |
title_sort | heterogeneity in the physiological states and pharmacological responses of differentiating 3t3-l1 preadipocytes |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779244/ https://www.ncbi.nlm.nih.gov/pubmed/19948481 http://dx.doi.org/10.1083/jcb.200904140 |
work_keys_str_mv | AT loolithsin heterogeneityinthephysiologicalstatesandpharmacologicalresponsesofdifferentiating3t3l1preadipocytes AT linhaijui heterogeneityinthephysiologicalstatesandpharmacologicalresponsesofdifferentiating3t3l1preadipocytes AT singhdineshk heterogeneityinthephysiologicalstatesandpharmacologicalresponsesofdifferentiating3t3l1preadipocytes AT lyonskathleenm heterogeneityinthephysiologicalstatesandpharmacologicalresponsesofdifferentiating3t3l1preadipocytes AT altschulerstevenj heterogeneityinthephysiologicalstatesandpharmacologicalresponsesofdifferentiating3t3l1preadipocytes AT wulanif heterogeneityinthephysiologicalstatesandpharmacologicalresponsesofdifferentiating3t3l1preadipocytes |