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Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast

Faithful segregation of sister chromatids requires the attachment of each kinetochore (Kt) to microtubules (MTs) that extend from opposite spindle poles. Merotelic Kt orientation is a Kt–MT misattachment in which a single Kt binds MTs from both spindle poles rather than just one. Genetic induction o...

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Detalles Bibliográficos
Autores principales: Courtheoux, Thibault, Gay, Guillaume, Gachet, Yannick, Tournier, Sylvie
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779255/
https://www.ncbi.nlm.nih.gov/pubmed/19948483
http://dx.doi.org/10.1083/jcb.200902093
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author Courtheoux, Thibault
Gay, Guillaume
Gachet, Yannick
Tournier, Sylvie
author_facet Courtheoux, Thibault
Gay, Guillaume
Gachet, Yannick
Tournier, Sylvie
author_sort Courtheoux, Thibault
collection PubMed
description Faithful segregation of sister chromatids requires the attachment of each kinetochore (Kt) to microtubules (MTs) that extend from opposite spindle poles. Merotelic Kt orientation is a Kt–MT misattachment in which a single Kt binds MTs from both spindle poles rather than just one. Genetic induction of merotelic Kt attachment during anaphase in fission yeast resulted in intra-Kt stretching followed by either correction or Kt disruption. Laser ablation of spindle MTs revealed that intra-Kt stretching and merotelic correction were dependent on MT forces. The presence of multiple merotelic chromosomes linearly antagonized the spindle elongation rate, and this phenomenon could be solved numerically using a simple force balance model. Based on the predictions of our mechanical model, we provide in vivo evidence that correction of merotelic attachment in anaphase is tension dependent and requires an Ase1/Prc1-dependent mechanism that prevents spindle collapse and thus asymmetric division and/or the appearance of the cut phenotype.
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spelling pubmed-27792552010-05-02 Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast Courtheoux, Thibault Gay, Guillaume Gachet, Yannick Tournier, Sylvie J Cell Biol Research Articles Faithful segregation of sister chromatids requires the attachment of each kinetochore (Kt) to microtubules (MTs) that extend from opposite spindle poles. Merotelic Kt orientation is a Kt–MT misattachment in which a single Kt binds MTs from both spindle poles rather than just one. Genetic induction of merotelic Kt attachment during anaphase in fission yeast resulted in intra-Kt stretching followed by either correction or Kt disruption. Laser ablation of spindle MTs revealed that intra-Kt stretching and merotelic correction were dependent on MT forces. The presence of multiple merotelic chromosomes linearly antagonized the spindle elongation rate, and this phenomenon could be solved numerically using a simple force balance model. Based on the predictions of our mechanical model, we provide in vivo evidence that correction of merotelic attachment in anaphase is tension dependent and requires an Ase1/Prc1-dependent mechanism that prevents spindle collapse and thus asymmetric division and/or the appearance of the cut phenotype. The Rockefeller University Press 2009-11-02 /pmc/articles/PMC2779255/ /pubmed/19948483 http://dx.doi.org/10.1083/jcb.200902093 Text en © 2009 Courtheoux et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Courtheoux, Thibault
Gay, Guillaume
Gachet, Yannick
Tournier, Sylvie
Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast
title Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast
title_full Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast
title_fullStr Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast
title_full_unstemmed Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast
title_short Ase1/Prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast
title_sort ase1/prc1-dependent spindle elongation corrects merotely during anaphase in fission yeast
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779255/
https://www.ncbi.nlm.nih.gov/pubmed/19948483
http://dx.doi.org/10.1083/jcb.200902093
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