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Fail-Safe Transcriptional Termination for Protein-Coding Genes in S. cerevisiae

Transcription termination of RNA polymerase II (Pol II) on protein-coding genes in S. cerevisiae relies on pA site recognition by 3′ end processing factors. Here we demonstrate the existence of two alternative termination mechanisms that rescue polymerases failing to disengage from the template at p...

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Detalles Bibliográficos
Autores principales: Rondón, Ana G., Mischo, Hannah E., Kawauchi, Junya, Proudfoot, Nick J.
Formato: Texto
Lenguaje:English
Publicado: Cell Press 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779338/
https://www.ncbi.nlm.nih.gov/pubmed/19818712
http://dx.doi.org/10.1016/j.molcel.2009.07.028
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author Rondón, Ana G.
Mischo, Hannah E.
Kawauchi, Junya
Proudfoot, Nick J.
author_facet Rondón, Ana G.
Mischo, Hannah E.
Kawauchi, Junya
Proudfoot, Nick J.
author_sort Rondón, Ana G.
collection PubMed
description Transcription termination of RNA polymerase II (Pol II) on protein-coding genes in S. cerevisiae relies on pA site recognition by 3′ end processing factors. Here we demonstrate the existence of two alternative termination mechanisms that rescue polymerases failing to disengage from the template at pA sites. One of these fail-safe mechanisms is mediated by the NRD complex, similar to termination of short noncoding genes. The other termination mechanism is mediated by Rnt1 cleavage of the nascent transcript. Both fail-safe termination mechanisms trigger degradation of readthrough transcripts by the exosome. However, Rnt1-mediated termination can also enhance the usage of weak pA signals and thereby generate functional mRNA. We propose that these alternative Pol II termination pathways serve the dual function of avoiding transcription interference and promoting rapid removal of aberrant transcripts.
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spelling pubmed-27793382009-11-23 Fail-Safe Transcriptional Termination for Protein-Coding Genes in S. cerevisiae Rondón, Ana G. Mischo, Hannah E. Kawauchi, Junya Proudfoot, Nick J. Mol Cell Article Transcription termination of RNA polymerase II (Pol II) on protein-coding genes in S. cerevisiae relies on pA site recognition by 3′ end processing factors. Here we demonstrate the existence of two alternative termination mechanisms that rescue polymerases failing to disengage from the template at pA sites. One of these fail-safe mechanisms is mediated by the NRD complex, similar to termination of short noncoding genes. The other termination mechanism is mediated by Rnt1 cleavage of the nascent transcript. Both fail-safe termination mechanisms trigger degradation of readthrough transcripts by the exosome. However, Rnt1-mediated termination can also enhance the usage of weak pA signals and thereby generate functional mRNA. We propose that these alternative Pol II termination pathways serve the dual function of avoiding transcription interference and promoting rapid removal of aberrant transcripts. Cell Press 2009-10-09 /pmc/articles/PMC2779338/ /pubmed/19818712 http://dx.doi.org/10.1016/j.molcel.2009.07.028 Text en © 2009 ELL & Excerpta Medica. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Article
Rondón, Ana G.
Mischo, Hannah E.
Kawauchi, Junya
Proudfoot, Nick J.
Fail-Safe Transcriptional Termination for Protein-Coding Genes in S. cerevisiae
title Fail-Safe Transcriptional Termination for Protein-Coding Genes in S. cerevisiae
title_full Fail-Safe Transcriptional Termination for Protein-Coding Genes in S. cerevisiae
title_fullStr Fail-Safe Transcriptional Termination for Protein-Coding Genes in S. cerevisiae
title_full_unstemmed Fail-Safe Transcriptional Termination for Protein-Coding Genes in S. cerevisiae
title_short Fail-Safe Transcriptional Termination for Protein-Coding Genes in S. cerevisiae
title_sort fail-safe transcriptional termination for protein-coding genes in s. cerevisiae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2779338/
https://www.ncbi.nlm.nih.gov/pubmed/19818712
http://dx.doi.org/10.1016/j.molcel.2009.07.028
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