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Simplified, Enhanced Protein Purification Using an Inducible, Autoprocessing Enzyme Tag

We introduce a new method for purifying recombinant proteins expressed in bacteria using a highly specific, inducible, self-cleaving protease tag. This tag is comprised of the Vibrio cholerae MARTX toxin cysteine protease domain (CPD), an autoprocessing enzyme that cleaves exclusively after a leucin...

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Detalles Bibliográficos
Autores principales:	Shen, Aimee, Lupardus, Patrick J., Morell, Montse, Ponder, Elizabeth L., Sadaghiani, A. Masoud, Garcia, K. Christopher, Bogyo, Matthew
Formato:	Texto
Lenguaje:	English
Publicado:	Public Library of Science 2009
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2780291/ https://www.ncbi.nlm.nih.gov/pubmed/19956581 http://dx.doi.org/10.1371/journal.pone.0008119

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