Cargando…
Gene expression profiling in sinonasal adenocarcinoma
BACKGROUND: Sinonasal adenocarcinomas are uncommon tumors which develop in the ethmoid sinus after exposure to wood dust. Although the etiology of these tumors is well defined, very little is known about their molecular basis and no diagnostic tool exists for their early detection in high-risk worke...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2780459/ https://www.ncbi.nlm.nih.gov/pubmed/19903339 http://dx.doi.org/10.1186/1755-8794-2-65 |
_version_ | 1782174489880035328 |
---|---|
author | Tripodi, Dominique Quéméner, Sylvia Renaudin, Karine Ferron, Christophe Malard, Olivier Guisle-Marsollier, Isabelle Sébille-Rivain, Véronique Verger, Christian Géraut, Christian Gratas-Rabbia-Ré, Catherine |
author_facet | Tripodi, Dominique Quéméner, Sylvia Renaudin, Karine Ferron, Christophe Malard, Olivier Guisle-Marsollier, Isabelle Sébille-Rivain, Véronique Verger, Christian Géraut, Christian Gratas-Rabbia-Ré, Catherine |
author_sort | Tripodi, Dominique |
collection | PubMed |
description | BACKGROUND: Sinonasal adenocarcinomas are uncommon tumors which develop in the ethmoid sinus after exposure to wood dust. Although the etiology of these tumors is well defined, very little is known about their molecular basis and no diagnostic tool exists for their early detection in high-risk workers. METHODS: To identify genes involved in this disease, we performed gene expression profiling using cancer-dedicated microarrays, on nine matched samples of sinonasal adenocarcinomas and non-tumor sinusal tissue. Microarray results were validated by quantitative RT-PCR and immunohistochemistry on two additional sets of tumors. RESULTS: Among the genes with significant differential expression we selected LGALS4, ACS5, CLU, SRI and CCT5 for further exploration. The overexpression of LGALS4, ACS5, SRI, CCT5 and the downregulation of CLU were confirmed by quantitative RT-PCR. Immunohistochemistry was performed for LGALS4 (Galectin 4), ACS5 (Acyl-CoA synthetase) and CLU (Clusterin) proteins: LGALS4 was highly up-regulated, particularly in the most differentiated tumors, while CLU was lost in all tumors. The expression of ACS5, was more heterogeneous and no correlation was observed with the tumor type. CONCLUSION: Within our microarray study in sinonasal adenocarcinoma we identified two proteins, LGALS4 and CLU, that were significantly differentially expressed in tumors compared to normal tissue. A further evaluation on a new set of tissues, including precancerous stages and low grade tumors, is necessary to evaluate the possibility of using them as diagnostic markers. |
format | Text |
id | pubmed-2780459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27804592009-11-21 Gene expression profiling in sinonasal adenocarcinoma Tripodi, Dominique Quéméner, Sylvia Renaudin, Karine Ferron, Christophe Malard, Olivier Guisle-Marsollier, Isabelle Sébille-Rivain, Véronique Verger, Christian Géraut, Christian Gratas-Rabbia-Ré, Catherine BMC Med Genomics Research article BACKGROUND: Sinonasal adenocarcinomas are uncommon tumors which develop in the ethmoid sinus after exposure to wood dust. Although the etiology of these tumors is well defined, very little is known about their molecular basis and no diagnostic tool exists for their early detection in high-risk workers. METHODS: To identify genes involved in this disease, we performed gene expression profiling using cancer-dedicated microarrays, on nine matched samples of sinonasal adenocarcinomas and non-tumor sinusal tissue. Microarray results were validated by quantitative RT-PCR and immunohistochemistry on two additional sets of tumors. RESULTS: Among the genes with significant differential expression we selected LGALS4, ACS5, CLU, SRI and CCT5 for further exploration. The overexpression of LGALS4, ACS5, SRI, CCT5 and the downregulation of CLU were confirmed by quantitative RT-PCR. Immunohistochemistry was performed for LGALS4 (Galectin 4), ACS5 (Acyl-CoA synthetase) and CLU (Clusterin) proteins: LGALS4 was highly up-regulated, particularly in the most differentiated tumors, while CLU was lost in all tumors. The expression of ACS5, was more heterogeneous and no correlation was observed with the tumor type. CONCLUSION: Within our microarray study in sinonasal adenocarcinoma we identified two proteins, LGALS4 and CLU, that were significantly differentially expressed in tumors compared to normal tissue. A further evaluation on a new set of tissues, including precancerous stages and low grade tumors, is necessary to evaluate the possibility of using them as diagnostic markers. BioMed Central 2009-11-10 /pmc/articles/PMC2780459/ /pubmed/19903339 http://dx.doi.org/10.1186/1755-8794-2-65 Text en Copyright ©2009 Tripodi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research article Tripodi, Dominique Quéméner, Sylvia Renaudin, Karine Ferron, Christophe Malard, Olivier Guisle-Marsollier, Isabelle Sébille-Rivain, Véronique Verger, Christian Géraut, Christian Gratas-Rabbia-Ré, Catherine Gene expression profiling in sinonasal adenocarcinoma |
title | Gene expression profiling in sinonasal adenocarcinoma |
title_full | Gene expression profiling in sinonasal adenocarcinoma |
title_fullStr | Gene expression profiling in sinonasal adenocarcinoma |
title_full_unstemmed | Gene expression profiling in sinonasal adenocarcinoma |
title_short | Gene expression profiling in sinonasal adenocarcinoma |
title_sort | gene expression profiling in sinonasal adenocarcinoma |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2780459/ https://www.ncbi.nlm.nih.gov/pubmed/19903339 http://dx.doi.org/10.1186/1755-8794-2-65 |
work_keys_str_mv | AT tripodidominique geneexpressionprofilinginsinonasaladenocarcinoma AT quemenersylvia geneexpressionprofilinginsinonasaladenocarcinoma AT renaudinkarine geneexpressionprofilinginsinonasaladenocarcinoma AT ferronchristophe geneexpressionprofilinginsinonasaladenocarcinoma AT malardolivier geneexpressionprofilinginsinonasaladenocarcinoma AT guislemarsollierisabelle geneexpressionprofilinginsinonasaladenocarcinoma AT sebillerivainveronique geneexpressionprofilinginsinonasaladenocarcinoma AT vergerchristian geneexpressionprofilinginsinonasaladenocarcinoma AT gerautchristian geneexpressionprofilinginsinonasaladenocarcinoma AT gratasrabbiarecatherine geneexpressionprofilinginsinonasaladenocarcinoma |