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Red-Mediated Transposition and Final Release of the Mini-F Vector of a Cloned Infectious Herpesvirus Genome
Bacterial artificial chromosomes (BACs) are well-established cloning vehicles for functional genomics and for constructing targeting vectors and infectious viral DNA clones. Red-recombination-based mutagenesis techniques have enabled the manipulation of BACs in Escherichia coli without any remaining...
Autores principales: | Wussow, Felix, Fickenscher, Helmut, Tischer, B. Karsten |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2780728/ https://www.ncbi.nlm.nih.gov/pubmed/19997639 http://dx.doi.org/10.1371/journal.pone.0008178 |
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