Cargando…

Comprehensive Analysis of Inflammatory Immune Mediators in Vitreoretinal Diseases

Inflammation affects the formation and the progression of various vitreoretinal diseases. We performed a comprehensive analysis of inflammatory immune mediators in the vitreous fluids from total of 345 patients with diabetic macular edema (DME, n = 92), proliferative diabetic retinopathy (PDR, n = 1...

Descripción completa

Detalles Bibliográficos
Autores principales: Yoshimura, Takeru, Sonoda, Koh-Hei, Sugahara, Mika, Mochizuki, Yasutaka, Enaida, Hiroshi, Oshima, Yuji, Ueno, Akifumi, Hata, Yasuaki, Yoshida, Hiroki, Ishibashi, Tatsuro
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2780733/
https://www.ncbi.nlm.nih.gov/pubmed/19997642
http://dx.doi.org/10.1371/journal.pone.0008158
_version_ 1782174527471484928
author Yoshimura, Takeru
Sonoda, Koh-Hei
Sugahara, Mika
Mochizuki, Yasutaka
Enaida, Hiroshi
Oshima, Yuji
Ueno, Akifumi
Hata, Yasuaki
Yoshida, Hiroki
Ishibashi, Tatsuro
author_facet Yoshimura, Takeru
Sonoda, Koh-Hei
Sugahara, Mika
Mochizuki, Yasutaka
Enaida, Hiroshi
Oshima, Yuji
Ueno, Akifumi
Hata, Yasuaki
Yoshida, Hiroki
Ishibashi, Tatsuro
author_sort Yoshimura, Takeru
collection PubMed
description Inflammation affects the formation and the progression of various vitreoretinal diseases. We performed a comprehensive analysis of inflammatory immune mediators in the vitreous fluids from total of 345 patients with diabetic macular edema (DME, n = 92), proliferative diabetic retinopathy (PDR, n = 147), branch retinal vein occlusion (BRVO, n = 30), central retinal vein occlusion (CRVO, n = 13) and rhegmatogenous retinal detachment (RRD, n = 63). As a control, we selected a total of 83 patients with either idiopathic macular hole (MH) or idiopathic epiretinal membrane (ERM) that were free of major pathogenic intraocular changes, such as ischemic retina and proliferative membranes. The concentrations of 20 soluble factors (nine cytokines, six chemokines, and five growth factors) were measured simultaneously by multiplex bead analysis system. Out of 20 soluble factors, three factors: interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemoattractant protein-1 (MCP-1) were significantly elevated in all groups of vitreoretinal diseases (DME, PDR, BRVO, CRVO, and RRD) compared with control group. According to the correlation analysis in the individual patient's level, these three factors that were simultaneously increased, did not show any independent upregulation in all the examined diseases. Vascular endothelial growth factor (VEGF) was significantly elevated in patients with PDR and CRVO. In PDR patients, the elevation of VEGF was significantly correlated with the three factors: IL-6, IL-8, and MCP-1, while no significant correlation was observed in CRVO patients. In conclusion, multiplex bead system enabled a comprehensive soluble factor analysis in vitreous fluid derived from variety of patients. Major three factors: IL-6, IL-8, and MCP-1 were strongly correlated with each other indicating a common pathway involved in inflammation process in vitreoretinal diseases.
format Text
id pubmed-2780733
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-27807332009-12-08 Comprehensive Analysis of Inflammatory Immune Mediators in Vitreoretinal Diseases Yoshimura, Takeru Sonoda, Koh-Hei Sugahara, Mika Mochizuki, Yasutaka Enaida, Hiroshi Oshima, Yuji Ueno, Akifumi Hata, Yasuaki Yoshida, Hiroki Ishibashi, Tatsuro PLoS One Research Article Inflammation affects the formation and the progression of various vitreoretinal diseases. We performed a comprehensive analysis of inflammatory immune mediators in the vitreous fluids from total of 345 patients with diabetic macular edema (DME, n = 92), proliferative diabetic retinopathy (PDR, n = 147), branch retinal vein occlusion (BRVO, n = 30), central retinal vein occlusion (CRVO, n = 13) and rhegmatogenous retinal detachment (RRD, n = 63). As a control, we selected a total of 83 patients with either idiopathic macular hole (MH) or idiopathic epiretinal membrane (ERM) that were free of major pathogenic intraocular changes, such as ischemic retina and proliferative membranes. The concentrations of 20 soluble factors (nine cytokines, six chemokines, and five growth factors) were measured simultaneously by multiplex bead analysis system. Out of 20 soluble factors, three factors: interleukin-6 (IL-6), interleukin-8 (IL-8), and monocyte chemoattractant protein-1 (MCP-1) were significantly elevated in all groups of vitreoretinal diseases (DME, PDR, BRVO, CRVO, and RRD) compared with control group. According to the correlation analysis in the individual patient's level, these three factors that were simultaneously increased, did not show any independent upregulation in all the examined diseases. Vascular endothelial growth factor (VEGF) was significantly elevated in patients with PDR and CRVO. In PDR patients, the elevation of VEGF was significantly correlated with the three factors: IL-6, IL-8, and MCP-1, while no significant correlation was observed in CRVO patients. In conclusion, multiplex bead system enabled a comprehensive soluble factor analysis in vitreous fluid derived from variety of patients. Major three factors: IL-6, IL-8, and MCP-1 were strongly correlated with each other indicating a common pathway involved in inflammation process in vitreoretinal diseases. Public Library of Science 2009-12-04 /pmc/articles/PMC2780733/ /pubmed/19997642 http://dx.doi.org/10.1371/journal.pone.0008158 Text en Yoshimura et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Yoshimura, Takeru
Sonoda, Koh-Hei
Sugahara, Mika
Mochizuki, Yasutaka
Enaida, Hiroshi
Oshima, Yuji
Ueno, Akifumi
Hata, Yasuaki
Yoshida, Hiroki
Ishibashi, Tatsuro
Comprehensive Analysis of Inflammatory Immune Mediators in Vitreoretinal Diseases
title Comprehensive Analysis of Inflammatory Immune Mediators in Vitreoretinal Diseases
title_full Comprehensive Analysis of Inflammatory Immune Mediators in Vitreoretinal Diseases
title_fullStr Comprehensive Analysis of Inflammatory Immune Mediators in Vitreoretinal Diseases
title_full_unstemmed Comprehensive Analysis of Inflammatory Immune Mediators in Vitreoretinal Diseases
title_short Comprehensive Analysis of Inflammatory Immune Mediators in Vitreoretinal Diseases
title_sort comprehensive analysis of inflammatory immune mediators in vitreoretinal diseases
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2780733/
https://www.ncbi.nlm.nih.gov/pubmed/19997642
http://dx.doi.org/10.1371/journal.pone.0008158
work_keys_str_mv AT yoshimuratakeru comprehensiveanalysisofinflammatoryimmunemediatorsinvitreoretinaldiseases
AT sonodakohhei comprehensiveanalysisofinflammatoryimmunemediatorsinvitreoretinaldiseases
AT sugaharamika comprehensiveanalysisofinflammatoryimmunemediatorsinvitreoretinaldiseases
AT mochizukiyasutaka comprehensiveanalysisofinflammatoryimmunemediatorsinvitreoretinaldiseases
AT enaidahiroshi comprehensiveanalysisofinflammatoryimmunemediatorsinvitreoretinaldiseases
AT oshimayuji comprehensiveanalysisofinflammatoryimmunemediatorsinvitreoretinaldiseases
AT uenoakifumi comprehensiveanalysisofinflammatoryimmunemediatorsinvitreoretinaldiseases
AT hatayasuaki comprehensiveanalysisofinflammatoryimmunemediatorsinvitreoretinaldiseases
AT yoshidahiroki comprehensiveanalysisofinflammatoryimmunemediatorsinvitreoretinaldiseases
AT ishibashitatsuro comprehensiveanalysisofinflammatoryimmunemediatorsinvitreoretinaldiseases