Cargando…
Assessment of antimalarial activity against Plasmodium falciparum and phytochemical screening of some Yemeni medicinal plants
Developing countries, where malaria is one of the most prevalent diseases, still rely on traditional medicine as a source for the treatment of this disease. In the present study, six selected plants (Acalypha fruticosa, Azadirachta indica, Cissus rotundifolia, Echium rauwalfii, Dendrosicyos socotran...
Autores principales: | , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2781778/ https://www.ncbi.nlm.nih.gov/pubmed/18955251 http://dx.doi.org/10.1093/ecam/nem148 |
_version_ | 1782174586187546624 |
---|---|
author | Alshawsh, Mohammed A. Mothana, Ramzi A. Al-shamahy, Hassan A. Alsllami, Salah F. Lindequist, Ulrike |
author_facet | Alshawsh, Mohammed A. Mothana, Ramzi A. Al-shamahy, Hassan A. Alsllami, Salah F. Lindequist, Ulrike |
author_sort | Alshawsh, Mohammed A. |
collection | PubMed |
description | Developing countries, where malaria is one of the most prevalent diseases, still rely on traditional medicine as a source for the treatment of this disease. In the present study, six selected plants (Acalypha fruticosa, Azadirachta indica, Cissus rotundifolia, Echium rauwalfii, Dendrosicyos socotrana and Boswellia elongata) commonly used in Yemen by traditional healers for the treatment of malaria as well as other diseases, were collected from different localities of Yemen, dried and extracted with methanol and water successfully. The antiplasmodial activity of the extracts was evaluated against fresh clinical isolates of Plasmodium falciparum. The selectivity parameters to evaluate the efficacy of these medicinal plants were measured by in vitro micro test (Mark III) according to World Health Organization (WHO) 1996 & WHO 2001 protocols of antimalarial drug tests. Among the investigated 12 extracts, three were found to have significant antiplasmodial activity with IC(50) values less than 4 µg/ml, namely the water extracts of A. fruticosa, A. indica and D. socotrana. Six extracts showed moderate activity with IC(50) values ranging from 10 to 30 µg/ml and three appeared to be inactive with IC(50) values more than 30 µg/ml. In addition, preliminary phytochemical screening of the methanolic and aqueous extracts indicated the presence of saponins, tannins, flavonoids, terpenoids, polysaccharides and peptides. |
format | Text |
id | pubmed-2781778 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-27817782010-12-01 Assessment of antimalarial activity against Plasmodium falciparum and phytochemical screening of some Yemeni medicinal plants Alshawsh, Mohammed A. Mothana, Ramzi A. Al-shamahy, Hassan A. Alsllami, Salah F. Lindequist, Ulrike Evid Based Complement Alternat Med Original Articles - Basic Science Developing countries, where malaria is one of the most prevalent diseases, still rely on traditional medicine as a source for the treatment of this disease. In the present study, six selected plants (Acalypha fruticosa, Azadirachta indica, Cissus rotundifolia, Echium rauwalfii, Dendrosicyos socotrana and Boswellia elongata) commonly used in Yemen by traditional healers for the treatment of malaria as well as other diseases, were collected from different localities of Yemen, dried and extracted with methanol and water successfully. The antiplasmodial activity of the extracts was evaluated against fresh clinical isolates of Plasmodium falciparum. The selectivity parameters to evaluate the efficacy of these medicinal plants were measured by in vitro micro test (Mark III) according to World Health Organization (WHO) 1996 & WHO 2001 protocols of antimalarial drug tests. Among the investigated 12 extracts, three were found to have significant antiplasmodial activity with IC(50) values less than 4 µg/ml, namely the water extracts of A. fruticosa, A. indica and D. socotrana. Six extracts showed moderate activity with IC(50) values ranging from 10 to 30 µg/ml and three appeared to be inactive with IC(50) values more than 30 µg/ml. In addition, preliminary phytochemical screening of the methanolic and aqueous extracts indicated the presence of saponins, tannins, flavonoids, terpenoids, polysaccharides and peptides. Oxford University Press 2009-12 2007-10-22 /pmc/articles/PMC2781778/ /pubmed/18955251 http://dx.doi.org/10.1093/ecam/nem148 Text en © 2007 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Articles - Basic Science Alshawsh, Mohammed A. Mothana, Ramzi A. Al-shamahy, Hassan A. Alsllami, Salah F. Lindequist, Ulrike Assessment of antimalarial activity against Plasmodium falciparum and phytochemical screening of some Yemeni medicinal plants |
title | Assessment of antimalarial activity against Plasmodium falciparum and phytochemical screening of some Yemeni medicinal plants |
title_full | Assessment of antimalarial activity against Plasmodium falciparum and phytochemical screening of some Yemeni medicinal plants |
title_fullStr | Assessment of antimalarial activity against Plasmodium falciparum and phytochemical screening of some Yemeni medicinal plants |
title_full_unstemmed | Assessment of antimalarial activity against Plasmodium falciparum and phytochemical screening of some Yemeni medicinal plants |
title_short | Assessment of antimalarial activity against Plasmodium falciparum and phytochemical screening of some Yemeni medicinal plants |
title_sort | assessment of antimalarial activity against plasmodium falciparum and phytochemical screening of some yemeni medicinal plants |
topic | Original Articles - Basic Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2781778/ https://www.ncbi.nlm.nih.gov/pubmed/18955251 http://dx.doi.org/10.1093/ecam/nem148 |
work_keys_str_mv | AT alshawshmohammeda assessmentofantimalarialactivityagainstplasmodiumfalciparumandphytochemicalscreeningofsomeyemenimedicinalplants AT mothanaramzia assessmentofantimalarialactivityagainstplasmodiumfalciparumandphytochemicalscreeningofsomeyemenimedicinalplants AT alshamahyhassana assessmentofantimalarialactivityagainstplasmodiumfalciparumandphytochemicalscreeningofsomeyemenimedicinalplants AT alsllamisalahf assessmentofantimalarialactivityagainstplasmodiumfalciparumandphytochemicalscreeningofsomeyemenimedicinalplants AT lindequistulrike assessmentofantimalarialactivityagainstplasmodiumfalciparumandphytochemicalscreeningofsomeyemenimedicinalplants |