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Association of Epstein Barr Virus Infection (EBV) with Breast Cancer in Rural Indian Women

INTRODUCTION: Breast cancer is the most common malignancy affecting females worldwide but conventional risk factors are able to explain only a small proportion of these cases. A possible viral etiology for breast cancer has been proposed and Epstein-Barr Virus (EBV) is a widely researched candidate...

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Autores principales: Joshi, Deepti, Quadri, Munira, Gangane, Neha, Joshi, Rajnish, Gangane, Nitin
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782138/
https://www.ncbi.nlm.nih.gov/pubmed/19997605
http://dx.doi.org/10.1371/journal.pone.0008180
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author Joshi, Deepti
Quadri, Munira
Gangane, Neha
Joshi, Rajnish
Gangane, Nitin
author_facet Joshi, Deepti
Quadri, Munira
Gangane, Neha
Joshi, Rajnish
Gangane, Nitin
author_sort Joshi, Deepti
collection PubMed
description INTRODUCTION: Breast cancer is the most common malignancy affecting females worldwide but conventional risk factors are able to explain only a small proportion of these cases. A possible viral etiology for breast cancer has been proposed and Epstein-Barr Virus (EBV) is a widely researched candidate virus. The aim of the present study, first one of its kind from India, was to determine if there is a greater association of EBV infection with breast cancer patients as compared to patients with benign breast diseases. METHODS: We looked for expression of Epstein-Barr Virus Nuclear Antigen-1 (EBNA-1) in breast cancer tissue specimens by employing immunohistochemistry (IHC). We also measured levels of anti-EBNA-1 Immunoglobulin (IgG) antibodies in stored sera of these patients using commercial Enzyme linked Immunosorbent Assay (ELISA) kit. Patients with benign breast diseases were used as a comparison group for both immunohistochemical and serological analysis. RESULTS: 58 cases of malignant breast disease and 63 of benign breast disease (controls) were included in the study. Using manufacturer determined cut-off of 3 IU/ml, 50/55 tested (90.9%) cases and 27/33 tested (81.8%) controls were seropositive for anti-EBNA-1 IgG. Mean antibody levels were significantly higher for cases (54.22 IU/ml) as compared to controls (18.68 IU/ml). IHC for EBNA-1 was positive in 28/51 cases (54.9%). No IHC positivity was noted in the tested 30 controls. Our results show that EBNA-1 expression is seen in a significant proportion of breast cancer tissue specimens from rural India and as compared to patients with benign breast diseases these patients also have a higher immunological response against EBNA-1.
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spelling pubmed-27821382009-12-08 Association of Epstein Barr Virus Infection (EBV) with Breast Cancer in Rural Indian Women Joshi, Deepti Quadri, Munira Gangane, Neha Joshi, Rajnish Gangane, Nitin PLoS One Research Article INTRODUCTION: Breast cancer is the most common malignancy affecting females worldwide but conventional risk factors are able to explain only a small proportion of these cases. A possible viral etiology for breast cancer has been proposed and Epstein-Barr Virus (EBV) is a widely researched candidate virus. The aim of the present study, first one of its kind from India, was to determine if there is a greater association of EBV infection with breast cancer patients as compared to patients with benign breast diseases. METHODS: We looked for expression of Epstein-Barr Virus Nuclear Antigen-1 (EBNA-1) in breast cancer tissue specimens by employing immunohistochemistry (IHC). We also measured levels of anti-EBNA-1 Immunoglobulin (IgG) antibodies in stored sera of these patients using commercial Enzyme linked Immunosorbent Assay (ELISA) kit. Patients with benign breast diseases were used as a comparison group for both immunohistochemical and serological analysis. RESULTS: 58 cases of malignant breast disease and 63 of benign breast disease (controls) were included in the study. Using manufacturer determined cut-off of 3 IU/ml, 50/55 tested (90.9%) cases and 27/33 tested (81.8%) controls were seropositive for anti-EBNA-1 IgG. Mean antibody levels were significantly higher for cases (54.22 IU/ml) as compared to controls (18.68 IU/ml). IHC for EBNA-1 was positive in 28/51 cases (54.9%). No IHC positivity was noted in the tested 30 controls. Our results show that EBNA-1 expression is seen in a significant proportion of breast cancer tissue specimens from rural India and as compared to patients with benign breast diseases these patients also have a higher immunological response against EBNA-1. Public Library of Science 2009-12-04 /pmc/articles/PMC2782138/ /pubmed/19997605 http://dx.doi.org/10.1371/journal.pone.0008180 Text en Joshi et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Joshi, Deepti
Quadri, Munira
Gangane, Neha
Joshi, Rajnish
Gangane, Nitin
Association of Epstein Barr Virus Infection (EBV) with Breast Cancer in Rural Indian Women
title Association of Epstein Barr Virus Infection (EBV) with Breast Cancer in Rural Indian Women
title_full Association of Epstein Barr Virus Infection (EBV) with Breast Cancer in Rural Indian Women
title_fullStr Association of Epstein Barr Virus Infection (EBV) with Breast Cancer in Rural Indian Women
title_full_unstemmed Association of Epstein Barr Virus Infection (EBV) with Breast Cancer in Rural Indian Women
title_short Association of Epstein Barr Virus Infection (EBV) with Breast Cancer in Rural Indian Women
title_sort association of epstein barr virus infection (ebv) with breast cancer in rural indian women
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782138/
https://www.ncbi.nlm.nih.gov/pubmed/19997605
http://dx.doi.org/10.1371/journal.pone.0008180
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