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Molecular Mechanism for Human Sperm Chemotaxis Mediated by Progesterone
Sperm chemotaxis is a chemical guiding mechanism that may orient spermatozoa to the egg surface. A picomolar concentration gradient of Progesterone (P), the main steroidal component secreted by the cumulus cells that surround the egg, attracts human spermatozoa. In order to elucidate the molecular m...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782141/ https://www.ncbi.nlm.nih.gov/pubmed/19997608 http://dx.doi.org/10.1371/journal.pone.0008211 |
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author | Teves, Maria E. Guidobaldi, Hector A. Uñates, Diego R. Sanchez, Raul Miska, Werner Publicover, Stephen J. Morales Garcia, Aduén A. Giojalas, Laura C. |
author_facet | Teves, Maria E. Guidobaldi, Hector A. Uñates, Diego R. Sanchez, Raul Miska, Werner Publicover, Stephen J. Morales Garcia, Aduén A. Giojalas, Laura C. |
author_sort | Teves, Maria E. |
collection | PubMed |
description | Sperm chemotaxis is a chemical guiding mechanism that may orient spermatozoa to the egg surface. A picomolar concentration gradient of Progesterone (P), the main steroidal component secreted by the cumulus cells that surround the egg, attracts human spermatozoa. In order to elucidate the molecular mechanism of sperm chemotaxis mediated by P, we combine the application of different strategies: pharmacological inhibition of signaling molecules, measurements of the concentrations of second messengers and activation of the chemotactic signaling. Our data implicate a number of classic signal transduction pathways in the response and provide a model for the sequence of events, where the tmAC-cAMP-PKA pathway is activated first, followed by protein tyrosine phosphorylation (equatorial band and flagellum) and calcium mobilization (through IP(3)R and SOC channels), whereas the sGC-cGMP-PKG cascade, is activated later. These events lead to sperm orientation towards the source of the chemoattractant. The finding proposes a molecular mechanism which contributes to the understanding of the signal transduction pathway that takes place in a physiological process as chemotaxis. |
format | Text |
id | pubmed-2782141 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-27821412009-12-08 Molecular Mechanism for Human Sperm Chemotaxis Mediated by Progesterone Teves, Maria E. Guidobaldi, Hector A. Uñates, Diego R. Sanchez, Raul Miska, Werner Publicover, Stephen J. Morales Garcia, Aduén A. Giojalas, Laura C. PLoS One Research Article Sperm chemotaxis is a chemical guiding mechanism that may orient spermatozoa to the egg surface. A picomolar concentration gradient of Progesterone (P), the main steroidal component secreted by the cumulus cells that surround the egg, attracts human spermatozoa. In order to elucidate the molecular mechanism of sperm chemotaxis mediated by P, we combine the application of different strategies: pharmacological inhibition of signaling molecules, measurements of the concentrations of second messengers and activation of the chemotactic signaling. Our data implicate a number of classic signal transduction pathways in the response and provide a model for the sequence of events, where the tmAC-cAMP-PKA pathway is activated first, followed by protein tyrosine phosphorylation (equatorial band and flagellum) and calcium mobilization (through IP(3)R and SOC channels), whereas the sGC-cGMP-PKG cascade, is activated later. These events lead to sperm orientation towards the source of the chemoattractant. The finding proposes a molecular mechanism which contributes to the understanding of the signal transduction pathway that takes place in a physiological process as chemotaxis. Public Library of Science 2009-12-08 /pmc/articles/PMC2782141/ /pubmed/19997608 http://dx.doi.org/10.1371/journal.pone.0008211 Text en Teves et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Teves, Maria E. Guidobaldi, Hector A. Uñates, Diego R. Sanchez, Raul Miska, Werner Publicover, Stephen J. Morales Garcia, Aduén A. Giojalas, Laura C. Molecular Mechanism for Human Sperm Chemotaxis Mediated by Progesterone |
title | Molecular Mechanism for Human Sperm Chemotaxis Mediated by Progesterone |
title_full | Molecular Mechanism for Human Sperm Chemotaxis Mediated by Progesterone |
title_fullStr | Molecular Mechanism for Human Sperm Chemotaxis Mediated by Progesterone |
title_full_unstemmed | Molecular Mechanism for Human Sperm Chemotaxis Mediated by Progesterone |
title_short | Molecular Mechanism for Human Sperm Chemotaxis Mediated by Progesterone |
title_sort | molecular mechanism for human sperm chemotaxis mediated by progesterone |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782141/ https://www.ncbi.nlm.nih.gov/pubmed/19997608 http://dx.doi.org/10.1371/journal.pone.0008211 |
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