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The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells

Statins have been postulated to affect the bone metabolism. Recent experimental and epidemiologic studies have suggested that statins may also have bone protective effects. This study assessed the effects of simvastatin on the proliferation and differentiation of human bone marrow stromal cells (BMS...

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Autores principales: Baek, Ki Hyun, Lee, Won Young, Oh, Ki Won, Tae, Hyun Jung, Lee, Jung Min, Lee, En Jung, Han, Je Ho, Kang, Moo Il, Cha, Bong Yun, Lee, Kwang Woo, Son, Ho Young, Kang, Sung Koo
Formato: Texto
Lenguaje:English
Publicado: The Korean Academy of Medical Sciences 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782200/
https://www.ncbi.nlm.nih.gov/pubmed/15953866
http://dx.doi.org/10.3346/jkms.2005.20.3.438
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author Baek, Ki Hyun
Lee, Won Young
Oh, Ki Won
Tae, Hyun Jung
Lee, Jung Min
Lee, En Jung
Han, Je Ho
Kang, Moo Il
Cha, Bong Yun
Lee, Kwang Woo
Son, Ho Young
Kang, Sung Koo
author_facet Baek, Ki Hyun
Lee, Won Young
Oh, Ki Won
Tae, Hyun Jung
Lee, Jung Min
Lee, En Jung
Han, Je Ho
Kang, Moo Il
Cha, Bong Yun
Lee, Kwang Woo
Son, Ho Young
Kang, Sung Koo
author_sort Baek, Ki Hyun
collection PubMed
description Statins have been postulated to affect the bone metabolism. Recent experimental and epidemiologic studies have suggested that statins may also have bone protective effects. This study assessed the effects of simvastatin on the proliferation and differentiation of human bone marrow stromal cells (BMSCs) in an ex vivo culture. The bone marrow was obtained from healthy donors. Mononuclear cells were isolated and cultured to osteoblastic lineage. In the primary culture, 10(-6) M simvastatin diminished the mean size of the colony forming units-fibroblastic (CFU-Fs) and enhanced matrix calcification. At near confluence, the cells were sub-cultured. Thereafter, the alkaline phosphatase (ALP) activities of each group were measured by the time course of the secondary culture. Simvastatin increased the ALP activity in a dose dependent manner, and this stimulatory effect was more evident during the early period of culture. A 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay was performed during the secondary culture in order to estimate the effect of simvastatin on the proliferation of human BMSCs. When compared to the control group, simvastatin significantly decreased the proliferation of cells of each culture well. 10(-6) M of simvastatin also significantly enhanced the osteocalcin mRNA expression level. This study shows that simvastatin has a stimulatory effect on bone formation through osteoblastic differentiation, and has an inhibitory effect on the proliferative potential of human BMSCs
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spelling pubmed-27822002009-11-30 The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells Baek, Ki Hyun Lee, Won Young Oh, Ki Won Tae, Hyun Jung Lee, Jung Min Lee, En Jung Han, Je Ho Kang, Moo Il Cha, Bong Yun Lee, Kwang Woo Son, Ho Young Kang, Sung Koo J Korean Med Sci Original Article Statins have been postulated to affect the bone metabolism. Recent experimental and epidemiologic studies have suggested that statins may also have bone protective effects. This study assessed the effects of simvastatin on the proliferation and differentiation of human bone marrow stromal cells (BMSCs) in an ex vivo culture. The bone marrow was obtained from healthy donors. Mononuclear cells were isolated and cultured to osteoblastic lineage. In the primary culture, 10(-6) M simvastatin diminished the mean size of the colony forming units-fibroblastic (CFU-Fs) and enhanced matrix calcification. At near confluence, the cells were sub-cultured. Thereafter, the alkaline phosphatase (ALP) activities of each group were measured by the time course of the secondary culture. Simvastatin increased the ALP activity in a dose dependent manner, and this stimulatory effect was more evident during the early period of culture. A 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay was performed during the secondary culture in order to estimate the effect of simvastatin on the proliferation of human BMSCs. When compared to the control group, simvastatin significantly decreased the proliferation of cells of each culture well. 10(-6) M of simvastatin also significantly enhanced the osteocalcin mRNA expression level. This study shows that simvastatin has a stimulatory effect on bone formation through osteoblastic differentiation, and has an inhibitory effect on the proliferative potential of human BMSCs The Korean Academy of Medical Sciences 2005-06 2005-06-30 /pmc/articles/PMC2782200/ /pubmed/15953866 http://dx.doi.org/10.3346/jkms.2005.20.3.438 Text en Copyright © 2005 The Korean Academy of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Baek, Ki Hyun
Lee, Won Young
Oh, Ki Won
Tae, Hyun Jung
Lee, Jung Min
Lee, En Jung
Han, Je Ho
Kang, Moo Il
Cha, Bong Yun
Lee, Kwang Woo
Son, Ho Young
Kang, Sung Koo
The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells
title The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells
title_full The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells
title_fullStr The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells
title_full_unstemmed The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells
title_short The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells
title_sort effect of simvastatin on the proliferation and differentiation of human bone marrow stromal cells
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782200/
https://www.ncbi.nlm.nih.gov/pubmed/15953866
http://dx.doi.org/10.3346/jkms.2005.20.3.438
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