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The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells
Statins have been postulated to affect the bone metabolism. Recent experimental and epidemiologic studies have suggested that statins may also have bone protective effects. This study assessed the effects of simvastatin on the proliferation and differentiation of human bone marrow stromal cells (BMS...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Korean Academy of Medical Sciences
2005
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782200/ https://www.ncbi.nlm.nih.gov/pubmed/15953866 http://dx.doi.org/10.3346/jkms.2005.20.3.438 |
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author | Baek, Ki Hyun Lee, Won Young Oh, Ki Won Tae, Hyun Jung Lee, Jung Min Lee, En Jung Han, Je Ho Kang, Moo Il Cha, Bong Yun Lee, Kwang Woo Son, Ho Young Kang, Sung Koo |
author_facet | Baek, Ki Hyun Lee, Won Young Oh, Ki Won Tae, Hyun Jung Lee, Jung Min Lee, En Jung Han, Je Ho Kang, Moo Il Cha, Bong Yun Lee, Kwang Woo Son, Ho Young Kang, Sung Koo |
author_sort | Baek, Ki Hyun |
collection | PubMed |
description | Statins have been postulated to affect the bone metabolism. Recent experimental and epidemiologic studies have suggested that statins may also have bone protective effects. This study assessed the effects of simvastatin on the proliferation and differentiation of human bone marrow stromal cells (BMSCs) in an ex vivo culture. The bone marrow was obtained from healthy donors. Mononuclear cells were isolated and cultured to osteoblastic lineage. In the primary culture, 10(-6) M simvastatin diminished the mean size of the colony forming units-fibroblastic (CFU-Fs) and enhanced matrix calcification. At near confluence, the cells were sub-cultured. Thereafter, the alkaline phosphatase (ALP) activities of each group were measured by the time course of the secondary culture. Simvastatin increased the ALP activity in a dose dependent manner, and this stimulatory effect was more evident during the early period of culture. A 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay was performed during the secondary culture in order to estimate the effect of simvastatin on the proliferation of human BMSCs. When compared to the control group, simvastatin significantly decreased the proliferation of cells of each culture well. 10(-6) M of simvastatin also significantly enhanced the osteocalcin mRNA expression level. This study shows that simvastatin has a stimulatory effect on bone formation through osteoblastic differentiation, and has an inhibitory effect on the proliferative potential of human BMSCs |
format | Text |
id | pubmed-2782200 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Korean Academy of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-27822002009-11-30 The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells Baek, Ki Hyun Lee, Won Young Oh, Ki Won Tae, Hyun Jung Lee, Jung Min Lee, En Jung Han, Je Ho Kang, Moo Il Cha, Bong Yun Lee, Kwang Woo Son, Ho Young Kang, Sung Koo J Korean Med Sci Original Article Statins have been postulated to affect the bone metabolism. Recent experimental and epidemiologic studies have suggested that statins may also have bone protective effects. This study assessed the effects of simvastatin on the proliferation and differentiation of human bone marrow stromal cells (BMSCs) in an ex vivo culture. The bone marrow was obtained from healthy donors. Mononuclear cells were isolated and cultured to osteoblastic lineage. In the primary culture, 10(-6) M simvastatin diminished the mean size of the colony forming units-fibroblastic (CFU-Fs) and enhanced matrix calcification. At near confluence, the cells were sub-cultured. Thereafter, the alkaline phosphatase (ALP) activities of each group were measured by the time course of the secondary culture. Simvastatin increased the ALP activity in a dose dependent manner, and this stimulatory effect was more evident during the early period of culture. A 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay was performed during the secondary culture in order to estimate the effect of simvastatin on the proliferation of human BMSCs. When compared to the control group, simvastatin significantly decreased the proliferation of cells of each culture well. 10(-6) M of simvastatin also significantly enhanced the osteocalcin mRNA expression level. This study shows that simvastatin has a stimulatory effect on bone formation through osteoblastic differentiation, and has an inhibitory effect on the proliferative potential of human BMSCs The Korean Academy of Medical Sciences 2005-06 2005-06-30 /pmc/articles/PMC2782200/ /pubmed/15953866 http://dx.doi.org/10.3346/jkms.2005.20.3.438 Text en Copyright © 2005 The Korean Academy of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Baek, Ki Hyun Lee, Won Young Oh, Ki Won Tae, Hyun Jung Lee, Jung Min Lee, En Jung Han, Je Ho Kang, Moo Il Cha, Bong Yun Lee, Kwang Woo Son, Ho Young Kang, Sung Koo The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells |
title | The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells |
title_full | The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells |
title_fullStr | The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells |
title_full_unstemmed | The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells |
title_short | The Effect of Simvastatin on the Proliferation and Differentiation of Human Bone Marrow Stromal Cells |
title_sort | effect of simvastatin on the proliferation and differentiation of human bone marrow stromal cells |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782200/ https://www.ncbi.nlm.nih.gov/pubmed/15953866 http://dx.doi.org/10.3346/jkms.2005.20.3.438 |
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