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Tissue Engineered Vascularized Bone Formation Using in vivo Implanted Osteoblast-Polyglycolic acid Scaffold
Repair of skeletal defects with vascularized bone grafts has many advantages over non-vascularized free grafts, but the availability of these grafts is extremely limited. This study was designed to determine whether new vascularized bone could be engineered by transplantation of osteoblasts around e...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Korean Academy of Medical Sciences
2005
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782207/ https://www.ncbi.nlm.nih.gov/pubmed/15953873 http://dx.doi.org/10.3346/jkms.2005.20.3.479 |
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author | Kim, Woo Seob Kim, Han Koo |
author_facet | Kim, Woo Seob Kim, Han Koo |
author_sort | Kim, Woo Seob |
collection | PubMed |
description | Repair of skeletal defects with vascularized bone grafts has many advantages over non-vascularized free grafts, but the availability of these grafts is extremely limited. This study was designed to determine whether new vascularized bone could be engineered by transplantation of osteoblasts around existing vascular pedicles using biodegradable, synthetic polymer as a cell delivery vehicle. Cells were isolated from the periosteum of fetal bovine humerus, and then seeded onto non-woven multifilament, polyglycolic acid polymer. The polymers provided three dimensional support during in vitro culture. The cell-polymer constructs were maintained in vitro for two weeks and then implanted around the right femoral vessels of twelve athymic nude rats. The polymer templates without the cells were implanted around left femoral vessels of each mouse as a control. Twelve rats were sacrificed at the following intervals: three rats at six,and nine rats at nine weeks. New bone formation was evident in 10 out of the 12 periosteal-derived cell seeded implants. At six weeks, the tissue was primarily composed of what appeared both grossly and histologically to be cartilage enveloping small islands of osteoid. The degree of osteoid and bone formation progressed with time, as blood vessels invaded the tissue. This tissue ultimately underwent morphogenesis to become an organized trabeculated bone with a vascular pedicle. We believe that this technique may prove to be useful in the reconstruction of bony defect. |
format | Text |
id | pubmed-2782207 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2005 |
publisher | The Korean Academy of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-27822072009-11-30 Tissue Engineered Vascularized Bone Formation Using in vivo Implanted Osteoblast-Polyglycolic acid Scaffold Kim, Woo Seob Kim, Han Koo J Korean Med Sci Original Article Repair of skeletal defects with vascularized bone grafts has many advantages over non-vascularized free grafts, but the availability of these grafts is extremely limited. This study was designed to determine whether new vascularized bone could be engineered by transplantation of osteoblasts around existing vascular pedicles using biodegradable, synthetic polymer as a cell delivery vehicle. Cells were isolated from the periosteum of fetal bovine humerus, and then seeded onto non-woven multifilament, polyglycolic acid polymer. The polymers provided three dimensional support during in vitro culture. The cell-polymer constructs were maintained in vitro for two weeks and then implanted around the right femoral vessels of twelve athymic nude rats. The polymer templates without the cells were implanted around left femoral vessels of each mouse as a control. Twelve rats were sacrificed at the following intervals: three rats at six,and nine rats at nine weeks. New bone formation was evident in 10 out of the 12 periosteal-derived cell seeded implants. At six weeks, the tissue was primarily composed of what appeared both grossly and histologically to be cartilage enveloping small islands of osteoid. The degree of osteoid and bone formation progressed with time, as blood vessels invaded the tissue. This tissue ultimately underwent morphogenesis to become an organized trabeculated bone with a vascular pedicle. We believe that this technique may prove to be useful in the reconstruction of bony defect. The Korean Academy of Medical Sciences 2005-06 2005-06-30 /pmc/articles/PMC2782207/ /pubmed/15953873 http://dx.doi.org/10.3346/jkms.2005.20.3.479 Text en Copyright © 2005 The Korean Academy of Medical Sciences http://creativecommons.org/licenses/by-nc/3.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Kim, Woo Seob Kim, Han Koo Tissue Engineered Vascularized Bone Formation Using in vivo Implanted Osteoblast-Polyglycolic acid Scaffold |
title | Tissue Engineered Vascularized Bone Formation Using in vivo Implanted Osteoblast-Polyglycolic acid Scaffold |
title_full | Tissue Engineered Vascularized Bone Formation Using in vivo Implanted Osteoblast-Polyglycolic acid Scaffold |
title_fullStr | Tissue Engineered Vascularized Bone Formation Using in vivo Implanted Osteoblast-Polyglycolic acid Scaffold |
title_full_unstemmed | Tissue Engineered Vascularized Bone Formation Using in vivo Implanted Osteoblast-Polyglycolic acid Scaffold |
title_short | Tissue Engineered Vascularized Bone Formation Using in vivo Implanted Osteoblast-Polyglycolic acid Scaffold |
title_sort | tissue engineered vascularized bone formation using in vivo implanted osteoblast-polyglycolic acid scaffold |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2782207/ https://www.ncbi.nlm.nih.gov/pubmed/15953873 http://dx.doi.org/10.3346/jkms.2005.20.3.479 |
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