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Knockout of Ku86 accelerates cellular senescence induced by high NaCl

NaCl induces DNA breaks, thus leading to cellular senescence. Here we showed that Ku86 deficiency accelerated the high NaCl-induced cellular senescence. We find that 1) high NaCl induces rapid cellular senescence in Ku86 deficient (xrs5) cells, 2) Ku86 deficiency shortens lifespan of C. elegans in h...

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Detalles Bibliográficos
Autores principales: Dmitrieva, Natalia I., Chen, Hua Tang, Nussenzweig, André, Burg, Maurice B.
Formato: Texto
Lenguaje:English
Publicado: Impact Journals LLC 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2783634/
https://www.ncbi.nlm.nih.gov/pubmed/19946467
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author Dmitrieva, Natalia I.
Chen, Hua Tang
Nussenzweig, André
Burg, Maurice B.
author_facet Dmitrieva, Natalia I.
Chen, Hua Tang
Nussenzweig, André
Burg, Maurice B.
author_sort Dmitrieva, Natalia I.
collection PubMed
description NaCl induces DNA breaks, thus leading to cellular senescence. Here we showed that Ku86 deficiency accelerated the high NaCl-induced cellular senescence. We find that 1) high NaCl induces rapid cellular senescence in Ku86 deficient (xrs5) cells, 2) Ku86 deficiency shortens lifespan of C. elegans in high NaCl, and 3) cellular senescence is greatly accelerated in renal inner medullas of Ku86(-/-) mice. Further, although water balance is known to be compromised in old mice, this occurs at much earlier age in Ku86(-/-) mice. When subjected to mild water restriction, 3 month old Ku86(-/-), but not Ku86(+/+), mice rapidly become dehydrated as evidenced by decrease in body weight, increased production of antidiuretic hormone, increased urine osmolality and decreased urine volume. The deficiency in water balance does not occur in Ku86(+/+)mice until they are much older (14 months). We conclude that Ku86 deficiency accelerates high NaCl-induced cellular senescence, particularly in the renal medulla where NaCl normally is high.
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spelling pubmed-27836342009-11-26 Knockout of Ku86 accelerates cellular senescence induced by high NaCl Dmitrieva, Natalia I. Chen, Hua Tang Nussenzweig, André Burg, Maurice B. Aging (Albany NY) Research Article NaCl induces DNA breaks, thus leading to cellular senescence. Here we showed that Ku86 deficiency accelerated the high NaCl-induced cellular senescence. We find that 1) high NaCl induces rapid cellular senescence in Ku86 deficient (xrs5) cells, 2) Ku86 deficiency shortens lifespan of C. elegans in high NaCl, and 3) cellular senescence is greatly accelerated in renal inner medullas of Ku86(-/-) mice. Further, although water balance is known to be compromised in old mice, this occurs at much earlier age in Ku86(-/-) mice. When subjected to mild water restriction, 3 month old Ku86(-/-), but not Ku86(+/+), mice rapidly become dehydrated as evidenced by decrease in body weight, increased production of antidiuretic hormone, increased urine osmolality and decreased urine volume. The deficiency in water balance does not occur in Ku86(+/+)mice until they are much older (14 months). We conclude that Ku86 deficiency accelerates high NaCl-induced cellular senescence, particularly in the renal medulla where NaCl normally is high. Impact Journals LLC 2009-02-12 /pmc/articles/PMC2783634/ /pubmed/19946467 Text en Copyright: ©2009 Dmitrieva et al. http://creativecommons.org/licenses/by/2.5/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Dmitrieva, Natalia I.
Chen, Hua Tang
Nussenzweig, André
Burg, Maurice B.
Knockout of Ku86 accelerates cellular senescence induced by high NaCl
title Knockout of Ku86 accelerates cellular senescence induced by high NaCl
title_full Knockout of Ku86 accelerates cellular senescence induced by high NaCl
title_fullStr Knockout of Ku86 accelerates cellular senescence induced by high NaCl
title_full_unstemmed Knockout of Ku86 accelerates cellular senescence induced by high NaCl
title_short Knockout of Ku86 accelerates cellular senescence induced by high NaCl
title_sort knockout of ku86 accelerates cellular senescence induced by high nacl
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2783634/
https://www.ncbi.nlm.nih.gov/pubmed/19946467
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