Versatile P(acman) BAC Libraries for Transgenesis Studies in Drosophila melanogaster

We constructed Drosophila melanogaster BAC libraries with 21-kb and 83-kb inserts in the P(acman) system. Clones representing 12-fold coverage and encompassing more than 95% of annotated genes were mapped onto the reference genome. These clones can be integrated into predetermined attP sites in the...

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Detalles Bibliográficos
Autores principales: Venken, Koen J. T., Carlson, Joseph W., Schulze, Karen L., Pan, Hongling, He, Yuchun, Spokony, Rebecca, Wan, Kenneth H., Koriabine, Maxim, de Jong, Pieter J., White, Kevin P., Bellen, Hugo J., Hoskins, Roger A.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2784134/
https://www.ncbi.nlm.nih.gov/pubmed/19465919
http://dx.doi.org/10.1038/nmeth.1331
Descripción
Sumario:We constructed Drosophila melanogaster BAC libraries with 21-kb and 83-kb inserts in the P(acman) system. Clones representing 12-fold coverage and encompassing more than 95% of annotated genes were mapped onto the reference genome. These clones can be integrated into predetermined attP sites in the genome using ΦC31 integrase to rescue mutations. They can be modified through recombineering, for example to incorporate protein tags and assess expression patterns.

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