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High-resolution, long-term characterization of bacterial motility using optical tweezers

We present a single-cell motility assay, which allows the quantification of bacterial swimming in a well-controlled environment, for durations of up to an hour and with a temporal resolution higher than the flagellar rotation rates of ~100 Hz. The assay is based on an instrument combining optical tw...

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Detalles Bibliográficos
Autores principales: Min, Taejin L., Mears, Patrick J., Chubiz, Lon M., Rao, Christopher V., Golding, Ido, Chemla, Yann R.
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2784139/
https://www.ncbi.nlm.nih.gov/pubmed/19801991
http://dx.doi.org/10.1038/nmeth.1380
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author Min, Taejin L.
Mears, Patrick J.
Chubiz, Lon M.
Rao, Christopher V.
Golding, Ido
Chemla, Yann R.
author_facet Min, Taejin L.
Mears, Patrick J.
Chubiz, Lon M.
Rao, Christopher V.
Golding, Ido
Chemla, Yann R.
author_sort Min, Taejin L.
collection PubMed
description We present a single-cell motility assay, which allows the quantification of bacterial swimming in a well-controlled environment, for durations of up to an hour and with a temporal resolution higher than the flagellar rotation rates of ~100 Hz. The assay is based on an instrument combining optical tweezers, light and fluorescence microscopy, and a microfluidic chamber. Using this device we characterized the long-term statistics of the run-tumble time series in individual Escherichia coli cells. We also quantified higher-order features of bacterial swimming, such as changes in velocity and reversals of swimming direction.
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spelling pubmed-27841392010-05-01 High-resolution, long-term characterization of bacterial motility using optical tweezers Min, Taejin L. Mears, Patrick J. Chubiz, Lon M. Rao, Christopher V. Golding, Ido Chemla, Yann R. Nat Methods Article We present a single-cell motility assay, which allows the quantification of bacterial swimming in a well-controlled environment, for durations of up to an hour and with a temporal resolution higher than the flagellar rotation rates of ~100 Hz. The assay is based on an instrument combining optical tweezers, light and fluorescence microscopy, and a microfluidic chamber. Using this device we characterized the long-term statistics of the run-tumble time series in individual Escherichia coli cells. We also quantified higher-order features of bacterial swimming, such as changes in velocity and reversals of swimming direction. 2009-10-04 2009-11 /pmc/articles/PMC2784139/ /pubmed/19801991 http://dx.doi.org/10.1038/nmeth.1380 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Min, Taejin L.
Mears, Patrick J.
Chubiz, Lon M.
Rao, Christopher V.
Golding, Ido
Chemla, Yann R.
High-resolution, long-term characterization of bacterial motility using optical tweezers
title High-resolution, long-term characterization of bacterial motility using optical tweezers
title_full High-resolution, long-term characterization of bacterial motility using optical tweezers
title_fullStr High-resolution, long-term characterization of bacterial motility using optical tweezers
title_full_unstemmed High-resolution, long-term characterization of bacterial motility using optical tweezers
title_short High-resolution, long-term characterization of bacterial motility using optical tweezers
title_sort high-resolution, long-term characterization of bacterial motility using optical tweezers
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2784139/
https://www.ncbi.nlm.nih.gov/pubmed/19801991
http://dx.doi.org/10.1038/nmeth.1380
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