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High-resolution, long-term characterization of bacterial motility using optical tweezers
We present a single-cell motility assay, which allows the quantification of bacterial swimming in a well-controlled environment, for durations of up to an hour and with a temporal resolution higher than the flagellar rotation rates of ~100 Hz. The assay is based on an instrument combining optical tw...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2784139/ https://www.ncbi.nlm.nih.gov/pubmed/19801991 http://dx.doi.org/10.1038/nmeth.1380 |
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author | Min, Taejin L. Mears, Patrick J. Chubiz, Lon M. Rao, Christopher V. Golding, Ido Chemla, Yann R. |
author_facet | Min, Taejin L. Mears, Patrick J. Chubiz, Lon M. Rao, Christopher V. Golding, Ido Chemla, Yann R. |
author_sort | Min, Taejin L. |
collection | PubMed |
description | We present a single-cell motility assay, which allows the quantification of bacterial swimming in a well-controlled environment, for durations of up to an hour and with a temporal resolution higher than the flagellar rotation rates of ~100 Hz. The assay is based on an instrument combining optical tweezers, light and fluorescence microscopy, and a microfluidic chamber. Using this device we characterized the long-term statistics of the run-tumble time series in individual Escherichia coli cells. We also quantified higher-order features of bacterial swimming, such as changes in velocity and reversals of swimming direction. |
format | Text |
id | pubmed-2784139 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
record_format | MEDLINE/PubMed |
spelling | pubmed-27841392010-05-01 High-resolution, long-term characterization of bacterial motility using optical tweezers Min, Taejin L. Mears, Patrick J. Chubiz, Lon M. Rao, Christopher V. Golding, Ido Chemla, Yann R. Nat Methods Article We present a single-cell motility assay, which allows the quantification of bacterial swimming in a well-controlled environment, for durations of up to an hour and with a temporal resolution higher than the flagellar rotation rates of ~100 Hz. The assay is based on an instrument combining optical tweezers, light and fluorescence microscopy, and a microfluidic chamber. Using this device we characterized the long-term statistics of the run-tumble time series in individual Escherichia coli cells. We also quantified higher-order features of bacterial swimming, such as changes in velocity and reversals of swimming direction. 2009-10-04 2009-11 /pmc/articles/PMC2784139/ /pubmed/19801991 http://dx.doi.org/10.1038/nmeth.1380 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms |
spellingShingle | Article Min, Taejin L. Mears, Patrick J. Chubiz, Lon M. Rao, Christopher V. Golding, Ido Chemla, Yann R. High-resolution, long-term characterization of bacterial motility using optical tweezers |
title | High-resolution, long-term characterization of bacterial motility using optical tweezers |
title_full | High-resolution, long-term characterization of bacterial motility using optical tweezers |
title_fullStr | High-resolution, long-term characterization of bacterial motility using optical tweezers |
title_full_unstemmed | High-resolution, long-term characterization of bacterial motility using optical tweezers |
title_short | High-resolution, long-term characterization of bacterial motility using optical tweezers |
title_sort | high-resolution, long-term characterization of bacterial motility using optical tweezers |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2784139/ https://www.ncbi.nlm.nih.gov/pubmed/19801991 http://dx.doi.org/10.1038/nmeth.1380 |
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