MethMarker: user-friendly design and optimization of gene-specific DNA methylation assays

DNA methylation is a key mechanism of epigenetic regulation that is frequently altered in diseases such as cancer. To confirm the biological or clinical relevance of such changes, gene-specific DNA methylation changes need to be validated in multiple samples. We have developed the MethMarker http://...

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Detalles Bibliográficos
Autores principales: Schüffler, Peter, Mikeska, Thomas, Waha, Andreas, Lengauer, Thomas, Bock, Christoph
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Software
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2784320/
https://www.ncbi.nlm.nih.gov/pubmed/19804638
http://dx.doi.org/10.1186/gb-2009-10-10-r105
Descripción
Sumario:DNA methylation is a key mechanism of epigenetic regulation that is frequently altered in diseases such as cancer. To confirm the biological or clinical relevance of such changes, gene-specific DNA methylation changes need to be validated in multiple samples. We have developed the MethMarker http://methmarker.mpi-inf.mpg.de/ software to help design robust and cost-efficient DNA methylation assays for six widely used methods. Furthermore, MethMarker implements a bioinformatic workflow for transforming disease-specific differentially methylated genomic regions into robust clinical biomarkers.

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