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Why Hypothetical Protein KPN00728 of Klebsiella pneumoniae Should Be Classified as Chain C of Succinate Dehydrogenase?

Twenty percent of genes that encode for hypothetical proteins from Klebsiella pneumoniae MGH78578 were identified, leading to KPN00728 and KPN00729 after bioinformatics analysis. Both open reading frames showed high sequence homology to Succinate dehydrogenase Chain C (SdhC) and D (SdhD) from Escher...

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Autores principales: Choi, Sy Bing, Normi, Yahaya M., Wahab, Habibah A.
Formato: Texto
Lenguaje:English
Publicado: Springer US 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2785890/
https://www.ncbi.nlm.nih.gov/pubmed/19859792
http://dx.doi.org/10.1007/s10930-009-9209-9
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author Choi, Sy Bing
Normi, Yahaya M.
Wahab, Habibah A.
author_facet Choi, Sy Bing
Normi, Yahaya M.
Wahab, Habibah A.
author_sort Choi, Sy Bing
collection PubMed
description Twenty percent of genes that encode for hypothetical proteins from Klebsiella pneumoniae MGH78578 were identified, leading to KPN00728 and KPN00729 after bioinformatics analysis. Both open reading frames showed high sequence homology to Succinate dehydrogenase Chain C (SdhC) and D (SdhD) from Escherichia coli. Recently, KPN00729 was assigned as SdhD. KPN00728 thus remains of particular interest as no annotated genes from the complete genome sequence encode for SdhC. We discovered KPN00728 has a missing region with conserved residues important for ubiquinone (UQ) and heme group binding. Structure and function prediction of KPN00728 coupled with secondary structure analysis and transmembrane topology showed KPN00728 adopts SDH-(subunit C)-like structure. To further probe its functionality, UQ was docked on the built model (consisting KPN00728 and KPN00729) and formation of hydrogen bonds between UQ and Ser27, Arg31 (KPN00728) and Tyr84 (KPN00729) further reinforces and supports that KPN00728 is indeed SDH. This is the first report on the structural and function prediction of KPN00728 of K. pneumoniae MGH78578 as SdhC.
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spelling pubmed-27858902009-12-04 Why Hypothetical Protein KPN00728 of Klebsiella pneumoniae Should Be Classified as Chain C of Succinate Dehydrogenase? Choi, Sy Bing Normi, Yahaya M. Wahab, Habibah A. Protein J Article Twenty percent of genes that encode for hypothetical proteins from Klebsiella pneumoniae MGH78578 were identified, leading to KPN00728 and KPN00729 after bioinformatics analysis. Both open reading frames showed high sequence homology to Succinate dehydrogenase Chain C (SdhC) and D (SdhD) from Escherichia coli. Recently, KPN00729 was assigned as SdhD. KPN00728 thus remains of particular interest as no annotated genes from the complete genome sequence encode for SdhC. We discovered KPN00728 has a missing region with conserved residues important for ubiquinone (UQ) and heme group binding. Structure and function prediction of KPN00728 coupled with secondary structure analysis and transmembrane topology showed KPN00728 adopts SDH-(subunit C)-like structure. To further probe its functionality, UQ was docked on the built model (consisting KPN00728 and KPN00729) and formation of hydrogen bonds between UQ and Ser27, Arg31 (KPN00728) and Tyr84 (KPN00729) further reinforces and supports that KPN00728 is indeed SDH. This is the first report on the structural and function prediction of KPN00728 of K. pneumoniae MGH78578 as SdhC. Springer US 2009-10-27 2009 /pmc/articles/PMC2785890/ /pubmed/19859792 http://dx.doi.org/10.1007/s10930-009-9209-9 Text en © The Author(s) 2009 https://creativecommons.org/licenses/by-nc/4.0/ This article is distributed under the terms of the Creative Commons Attribution Noncommercial License which permits any noncommercial use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.
spellingShingle Article
Choi, Sy Bing
Normi, Yahaya M.
Wahab, Habibah A.
Why Hypothetical Protein KPN00728 of Klebsiella pneumoniae Should Be Classified as Chain C of Succinate Dehydrogenase?
title Why Hypothetical Protein KPN00728 of Klebsiella pneumoniae Should Be Classified as Chain C of Succinate Dehydrogenase?
title_full Why Hypothetical Protein KPN00728 of Klebsiella pneumoniae Should Be Classified as Chain C of Succinate Dehydrogenase?
title_fullStr Why Hypothetical Protein KPN00728 of Klebsiella pneumoniae Should Be Classified as Chain C of Succinate Dehydrogenase?
title_full_unstemmed Why Hypothetical Protein KPN00728 of Klebsiella pneumoniae Should Be Classified as Chain C of Succinate Dehydrogenase?
title_short Why Hypothetical Protein KPN00728 of Klebsiella pneumoniae Should Be Classified as Chain C of Succinate Dehydrogenase?
title_sort why hypothetical protein kpn00728 of klebsiella pneumoniae should be classified as chain c of succinate dehydrogenase?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2785890/
https://www.ncbi.nlm.nih.gov/pubmed/19859792
http://dx.doi.org/10.1007/s10930-009-9209-9
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