Cargando…
Plasma cytokine profiles in systemic sclerosis: associations with autoantibody subsets and clinical manifestations
INTRODUCTION: Systemic sclerosis (SSc) (scleroderma) is a complex autoimmune disease that clinically manifests as progressive fibrosis of the skin and internal organs. Anti-centromere antibodies (ACAs), anti-topoisomerase antibodies (ATAs), and anti-RNA polymerase III antibodies (ARAs) are three mut...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2009
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787259/ https://www.ncbi.nlm.nih.gov/pubmed/19799786 http://dx.doi.org/10.1186/ar2821 |
_version_ | 1782174893709721600 |
---|---|
author | Gourh, Pravitt Arnett, Frank C Assassi, Shervin Tan, Filemon K Huang, Mei Diekman, Laura Mayes, Maureen D Reveille, John D Agarwal, Sandeep K |
author_facet | Gourh, Pravitt Arnett, Frank C Assassi, Shervin Tan, Filemon K Huang, Mei Diekman, Laura Mayes, Maureen D Reveille, John D Agarwal, Sandeep K |
author_sort | Gourh, Pravitt |
collection | PubMed |
description | INTRODUCTION: Systemic sclerosis (SSc) (scleroderma) is a complex autoimmune disease that clinically manifests as progressive fibrosis of the skin and internal organs. Anti-centromere antibodies (ACAs), anti-topoisomerase antibodies (ATAs), and anti-RNA polymerase III antibodies (ARAs) are three mutually exclusive SSc-associated autoantibodies that correlate with distinct clinical subsets characterized by extent of cutaneous involvement and pattern of organ involvement. The current report sought to determine whether plasma cytokine profiles differ in SSc patients grouped according to these SSc-associated autoantibody subsets. METHODS: Plasma from 444 SSc patients and 216 healthy controls was obtained from the Scleroderma Family Registry and University of Texas Rheumatology Division. Patients were classified according to the presence of ACAs, ATAs, ARAs, or none of the above (antibody-negative). Levels of 13 cytokines were determined using multiplex assays. RESULTS: Compared with females, healthy control males had higher plasma levels of IL-2 (P = 0.008), IL-5 (P = 0.01) and IL-8 (P = 0.01). In addition, in controls, IL-6 (P = 0.02) and IL-17 (P = 0.01) levels increased with advancing age. After adjusting for age and gender, SSc patients had higher circulating levels of TNFα (P < 0.0001), IL-6 (P < 0.0001), and IFNγ (P = 0.05) and lower IL-17 (P = 0.0005) and IL-23 (P = 0.014). Additional analyses demonstrated that disease duration also influenced these cytokine profiles. IL-6 was elevated in ATA-positive and ARA-positive patients, but not in ACA-positive patients. IL-8 was uniquely increased in the ATA-positive subset while both ATA-positive and ACA-positive subsets had elevated IFNγ and IL-10. IL-5 was only significantly increased in the ACA-positive subset. Lastly, patients with interstitial lung disease had elevated IL-6 and patients with pulmonary hypertension had elevated IL-6 and IL-13. CONCLUSIONS: Plasma cytokine profiles differ in SSc patients based on the presence of SSc-associated autoantibodies. Plasma cytokine profiles in SSc patients may also be affected by disease duration and the pattern of internal organ involvement. |
format | Text |
id | pubmed-2787259 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27872592009-12-02 Plasma cytokine profiles in systemic sclerosis: associations with autoantibody subsets and clinical manifestations Gourh, Pravitt Arnett, Frank C Assassi, Shervin Tan, Filemon K Huang, Mei Diekman, Laura Mayes, Maureen D Reveille, John D Agarwal, Sandeep K Arthritis Res Ther Research article INTRODUCTION: Systemic sclerosis (SSc) (scleroderma) is a complex autoimmune disease that clinically manifests as progressive fibrosis of the skin and internal organs. Anti-centromere antibodies (ACAs), anti-topoisomerase antibodies (ATAs), and anti-RNA polymerase III antibodies (ARAs) are three mutually exclusive SSc-associated autoantibodies that correlate with distinct clinical subsets characterized by extent of cutaneous involvement and pattern of organ involvement. The current report sought to determine whether plasma cytokine profiles differ in SSc patients grouped according to these SSc-associated autoantibody subsets. METHODS: Plasma from 444 SSc patients and 216 healthy controls was obtained from the Scleroderma Family Registry and University of Texas Rheumatology Division. Patients were classified according to the presence of ACAs, ATAs, ARAs, or none of the above (antibody-negative). Levels of 13 cytokines were determined using multiplex assays. RESULTS: Compared with females, healthy control males had higher plasma levels of IL-2 (P = 0.008), IL-5 (P = 0.01) and IL-8 (P = 0.01). In addition, in controls, IL-6 (P = 0.02) and IL-17 (P = 0.01) levels increased with advancing age. After adjusting for age and gender, SSc patients had higher circulating levels of TNFα (P < 0.0001), IL-6 (P < 0.0001), and IFNγ (P = 0.05) and lower IL-17 (P = 0.0005) and IL-23 (P = 0.014). Additional analyses demonstrated that disease duration also influenced these cytokine profiles. IL-6 was elevated in ATA-positive and ARA-positive patients, but not in ACA-positive patients. IL-8 was uniquely increased in the ATA-positive subset while both ATA-positive and ACA-positive subsets had elevated IFNγ and IL-10. IL-5 was only significantly increased in the ACA-positive subset. Lastly, patients with interstitial lung disease had elevated IL-6 and patients with pulmonary hypertension had elevated IL-6 and IL-13. CONCLUSIONS: Plasma cytokine profiles differ in SSc patients based on the presence of SSc-associated autoantibodies. Plasma cytokine profiles in SSc patients may also be affected by disease duration and the pattern of internal organ involvement. BioMed Central 2009 2009-10-02 /pmc/articles/PMC2787259/ /pubmed/19799786 http://dx.doi.org/10.1186/ar2821 Text en Copyright ©2009 Gourh et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research article Gourh, Pravitt Arnett, Frank C Assassi, Shervin Tan, Filemon K Huang, Mei Diekman, Laura Mayes, Maureen D Reveille, John D Agarwal, Sandeep K Plasma cytokine profiles in systemic sclerosis: associations with autoantibody subsets and clinical manifestations |
title | Plasma cytokine profiles in systemic sclerosis: associations with autoantibody subsets and clinical manifestations |
title_full | Plasma cytokine profiles in systemic sclerosis: associations with autoantibody subsets and clinical manifestations |
title_fullStr | Plasma cytokine profiles in systemic sclerosis: associations with autoantibody subsets and clinical manifestations |
title_full_unstemmed | Plasma cytokine profiles in systemic sclerosis: associations with autoantibody subsets and clinical manifestations |
title_short | Plasma cytokine profiles in systemic sclerosis: associations with autoantibody subsets and clinical manifestations |
title_sort | plasma cytokine profiles in systemic sclerosis: associations with autoantibody subsets and clinical manifestations |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787259/ https://www.ncbi.nlm.nih.gov/pubmed/19799786 http://dx.doi.org/10.1186/ar2821 |
work_keys_str_mv | AT gourhpravitt plasmacytokineprofilesinsystemicsclerosisassociationswithautoantibodysubsetsandclinicalmanifestations AT arnettfrankc plasmacytokineprofilesinsystemicsclerosisassociationswithautoantibodysubsetsandclinicalmanifestations AT assassishervin plasmacytokineprofilesinsystemicsclerosisassociationswithautoantibodysubsetsandclinicalmanifestations AT tanfilemonk plasmacytokineprofilesinsystemicsclerosisassociationswithautoantibodysubsetsandclinicalmanifestations AT huangmei plasmacytokineprofilesinsystemicsclerosisassociationswithautoantibodysubsetsandclinicalmanifestations AT diekmanlaura plasmacytokineprofilesinsystemicsclerosisassociationswithautoantibodysubsetsandclinicalmanifestations AT mayesmaureend plasmacytokineprofilesinsystemicsclerosisassociationswithautoantibodysubsetsandclinicalmanifestations AT reveillejohnd plasmacytokineprofilesinsystemicsclerosisassociationswithautoantibodysubsetsandclinicalmanifestations AT agarwalsandeepk plasmacytokineprofilesinsystemicsclerosisassociationswithautoantibodysubsetsandclinicalmanifestations |