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Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection
BACKGROUND: Staphylococcus aureus methicillin-resistant (MRSA) has been frequently isolated from endotracheal and lung puncture aspirates in malnourished children with pneumonia. In this work we evaluated the susceptibility of undernourished BALB/c mice and its ability to mount a protective immunity...
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787521/ https://www.ncbi.nlm.nih.gov/pubmed/19930660 http://dx.doi.org/10.1186/1471-2180-9-240 |
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author | França, Thais Graziela Donegá Ishikawa, Larissa Lumi Watanabe Zorzella-Pezavento, Sofia Fernanda Gonçalves Chiuso-Minicucci, Fernanda Guerino, Clara Pires Fujiara da Cunha, Maria de Lourdes Ribeiro de Souza Sartori, Alexandrina |
author_facet | França, Thais Graziela Donegá Ishikawa, Larissa Lumi Watanabe Zorzella-Pezavento, Sofia Fernanda Gonçalves Chiuso-Minicucci, Fernanda Guerino, Clara Pires Fujiara da Cunha, Maria de Lourdes Ribeiro de Souza Sartori, Alexandrina |
author_sort | França, Thais Graziela Donegá |
collection | PubMed |
description | BACKGROUND: Staphylococcus aureus methicillin-resistant (MRSA) has been frequently isolated from endotracheal and lung puncture aspirates in malnourished children with pneumonia. In this work we evaluated the susceptibility of undernourished BALB/c mice and its ability to mount a protective immunity against MRSA with emphasis on the lung involvement. RESULTS: BALB/c mice submitted to a 20% dietary restriction during 20 days presented a significant decrease in body weight, lymphocyte number and also atrophy in thymus and intestinal epithelium. Determination of bacterial load by the number of colony forming units (CFU) indicated a similar susceptibility whereas the findings of Gram stain clearly suggested a higher amount of bacteria in the lungs of normal mice than in the undernourished ones. Immunization reduced bacterial growth in the lungs of normal mice but not in the undernourished ones. Histopathological analysis showed that inflammation appeared in the lungs from normal mice only after infection and that immunization prevented this pulmonary inflammatory process. On the other hand, undernourished mice presented lung inflammation even before infection. In addition, the degree of this inflammatory process did not change with infection or previous immunization. CONCLUSION: Our results indicated that lung injury during MRSA infection is prevented by previous immunization in well nourished but not in undernourished mice. |
format | Text |
id | pubmed-2787521 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27875212009-12-03 Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection França, Thais Graziela Donegá Ishikawa, Larissa Lumi Watanabe Zorzella-Pezavento, Sofia Fernanda Gonçalves Chiuso-Minicucci, Fernanda Guerino, Clara Pires Fujiara da Cunha, Maria de Lourdes Ribeiro de Souza Sartori, Alexandrina BMC Microbiol Research article BACKGROUND: Staphylococcus aureus methicillin-resistant (MRSA) has been frequently isolated from endotracheal and lung puncture aspirates in malnourished children with pneumonia. In this work we evaluated the susceptibility of undernourished BALB/c mice and its ability to mount a protective immunity against MRSA with emphasis on the lung involvement. RESULTS: BALB/c mice submitted to a 20% dietary restriction during 20 days presented a significant decrease in body weight, lymphocyte number and also atrophy in thymus and intestinal epithelium. Determination of bacterial load by the number of colony forming units (CFU) indicated a similar susceptibility whereas the findings of Gram stain clearly suggested a higher amount of bacteria in the lungs of normal mice than in the undernourished ones. Immunization reduced bacterial growth in the lungs of normal mice but not in the undernourished ones. Histopathological analysis showed that inflammation appeared in the lungs from normal mice only after infection and that immunization prevented this pulmonary inflammatory process. On the other hand, undernourished mice presented lung inflammation even before infection. In addition, the degree of this inflammatory process did not change with infection or previous immunization. CONCLUSION: Our results indicated that lung injury during MRSA infection is prevented by previous immunization in well nourished but not in undernourished mice. BioMed Central 2009-11-23 /pmc/articles/PMC2787521/ /pubmed/19930660 http://dx.doi.org/10.1186/1471-2180-9-240 Text en Copyright ©2009 França et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research article França, Thais Graziela Donegá Ishikawa, Larissa Lumi Watanabe Zorzella-Pezavento, Sofia Fernanda Gonçalves Chiuso-Minicucci, Fernanda Guerino, Clara Pires Fujiara da Cunha, Maria de Lourdes Ribeiro de Souza Sartori, Alexandrina Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection |
title | Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection |
title_full | Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection |
title_fullStr | Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection |
title_full_unstemmed | Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection |
title_short | Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection |
title_sort | immunization protected well nourished mice but not undernourished ones from lung injury in methicillin-resistant staphylococcus aureus (mrsa) infection |
topic | Research article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787521/ https://www.ncbi.nlm.nih.gov/pubmed/19930660 http://dx.doi.org/10.1186/1471-2180-9-240 |
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