Cargando…

Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection

BACKGROUND: Staphylococcus aureus methicillin-resistant (MRSA) has been frequently isolated from endotracheal and lung puncture aspirates in malnourished children with pneumonia. In this work we evaluated the susceptibility of undernourished BALB/c mice and its ability to mount a protective immunity...

Descripción completa

Detalles Bibliográficos
Autores principales: França, Thais Graziela Donegá, Ishikawa, Larissa Lumi Watanabe, Zorzella-Pezavento, Sofia Fernanda Gonçalves, Chiuso-Minicucci, Fernanda, Guerino, Clara Pires Fujiara, da Cunha, Maria de Lourdes Ribeiro de Souza, Sartori, Alexandrina
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787521/
https://www.ncbi.nlm.nih.gov/pubmed/19930660
http://dx.doi.org/10.1186/1471-2180-9-240
_version_ 1782174915620765696
author França, Thais Graziela Donegá
Ishikawa, Larissa Lumi Watanabe
Zorzella-Pezavento, Sofia Fernanda Gonçalves
Chiuso-Minicucci, Fernanda
Guerino, Clara Pires Fujiara
da Cunha, Maria de Lourdes Ribeiro de Souza
Sartori, Alexandrina
author_facet França, Thais Graziela Donegá
Ishikawa, Larissa Lumi Watanabe
Zorzella-Pezavento, Sofia Fernanda Gonçalves
Chiuso-Minicucci, Fernanda
Guerino, Clara Pires Fujiara
da Cunha, Maria de Lourdes Ribeiro de Souza
Sartori, Alexandrina
author_sort França, Thais Graziela Donegá
collection PubMed
description BACKGROUND: Staphylococcus aureus methicillin-resistant (MRSA) has been frequently isolated from endotracheal and lung puncture aspirates in malnourished children with pneumonia. In this work we evaluated the susceptibility of undernourished BALB/c mice and its ability to mount a protective immunity against MRSA with emphasis on the lung involvement. RESULTS: BALB/c mice submitted to a 20% dietary restriction during 20 days presented a significant decrease in body weight, lymphocyte number and also atrophy in thymus and intestinal epithelium. Determination of bacterial load by the number of colony forming units (CFU) indicated a similar susceptibility whereas the findings of Gram stain clearly suggested a higher amount of bacteria in the lungs of normal mice than in the undernourished ones. Immunization reduced bacterial growth in the lungs of normal mice but not in the undernourished ones. Histopathological analysis showed that inflammation appeared in the lungs from normal mice only after infection and that immunization prevented this pulmonary inflammatory process. On the other hand, undernourished mice presented lung inflammation even before infection. In addition, the degree of this inflammatory process did not change with infection or previous immunization. CONCLUSION: Our results indicated that lung injury during MRSA infection is prevented by previous immunization in well nourished but not in undernourished mice.
format Text
id pubmed-2787521
institution National Center for Biotechnology Information
language English
publishDate 2009
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-27875212009-12-03 Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection França, Thais Graziela Donegá Ishikawa, Larissa Lumi Watanabe Zorzella-Pezavento, Sofia Fernanda Gonçalves Chiuso-Minicucci, Fernanda Guerino, Clara Pires Fujiara da Cunha, Maria de Lourdes Ribeiro de Souza Sartori, Alexandrina BMC Microbiol Research article BACKGROUND: Staphylococcus aureus methicillin-resistant (MRSA) has been frequently isolated from endotracheal and lung puncture aspirates in malnourished children with pneumonia. In this work we evaluated the susceptibility of undernourished BALB/c mice and its ability to mount a protective immunity against MRSA with emphasis on the lung involvement. RESULTS: BALB/c mice submitted to a 20% dietary restriction during 20 days presented a significant decrease in body weight, lymphocyte number and also atrophy in thymus and intestinal epithelium. Determination of bacterial load by the number of colony forming units (CFU) indicated a similar susceptibility whereas the findings of Gram stain clearly suggested a higher amount of bacteria in the lungs of normal mice than in the undernourished ones. Immunization reduced bacterial growth in the lungs of normal mice but not in the undernourished ones. Histopathological analysis showed that inflammation appeared in the lungs from normal mice only after infection and that immunization prevented this pulmonary inflammatory process. On the other hand, undernourished mice presented lung inflammation even before infection. In addition, the degree of this inflammatory process did not change with infection or previous immunization. CONCLUSION: Our results indicated that lung injury during MRSA infection is prevented by previous immunization in well nourished but not in undernourished mice. BioMed Central 2009-11-23 /pmc/articles/PMC2787521/ /pubmed/19930660 http://dx.doi.org/10.1186/1471-2180-9-240 Text en Copyright ©2009 França et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research article
França, Thais Graziela Donegá
Ishikawa, Larissa Lumi Watanabe
Zorzella-Pezavento, Sofia Fernanda Gonçalves
Chiuso-Minicucci, Fernanda
Guerino, Clara Pires Fujiara
da Cunha, Maria de Lourdes Ribeiro de Souza
Sartori, Alexandrina
Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection
title Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection
title_full Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection
title_fullStr Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection
title_full_unstemmed Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection
title_short Immunization protected well nourished mice but not undernourished ones from lung injury in Methicillin-resistant Staphylococcus aureus (MRSA) infection
title_sort immunization protected well nourished mice but not undernourished ones from lung injury in methicillin-resistant staphylococcus aureus (mrsa) infection
topic Research article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2787521/
https://www.ncbi.nlm.nih.gov/pubmed/19930660
http://dx.doi.org/10.1186/1471-2180-9-240
work_keys_str_mv AT francathaisgrazieladonega immunizationprotectedwellnourishedmicebutnotundernourishedonesfromlunginjuryinmethicillinresistantstaphylococcusaureusmrsainfection
AT ishikawalarissalumiwatanabe immunizationprotectedwellnourishedmicebutnotundernourishedonesfromlunginjuryinmethicillinresistantstaphylococcusaureusmrsainfection
AT zorzellapezaventosofiafernandagoncalves immunizationprotectedwellnourishedmicebutnotundernourishedonesfromlunginjuryinmethicillinresistantstaphylococcusaureusmrsainfection
AT chiusominicuccifernanda immunizationprotectedwellnourishedmicebutnotundernourishedonesfromlunginjuryinmethicillinresistantstaphylococcusaureusmrsainfection
AT guerinoclarapiresfujiara immunizationprotectedwellnourishedmicebutnotundernourishedonesfromlunginjuryinmethicillinresistantstaphylococcusaureusmrsainfection
AT dacunhamariadelourdesribeirodesouza immunizationprotectedwellnourishedmicebutnotundernourishedonesfromlunginjuryinmethicillinresistantstaphylococcusaureusmrsainfection
AT sartorialexandrina immunizationprotectedwellnourishedmicebutnotundernourishedonesfromlunginjuryinmethicillinresistantstaphylococcusaureusmrsainfection