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Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS
BACKGROUND: Dendritic cells (DCs) are the sentinels of the mammalian immune system, characterized by a complex maturation process driven by pathogen detection. Although multiple studies have described the analysis of activated DCs by transcriptional profiling, recent findings indicate that mRNAs are...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2788525/ https://www.ncbi.nlm.nih.gov/pubmed/19943945 http://dx.doi.org/10.1186/1745-7580-5-5 |
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author | Ceppi, Maurizio Clavarino, Giovanna Gatti, Evelina Schmidt, Enrico K de Gassart, Aude Blankenship, Derek Ogola, Gerald Banchereau, Jacques Chaussabel, Damien Pierre, Philippe |
author_facet | Ceppi, Maurizio Clavarino, Giovanna Gatti, Evelina Schmidt, Enrico K de Gassart, Aude Blankenship, Derek Ogola, Gerald Banchereau, Jacques Chaussabel, Damien Pierre, Philippe |
author_sort | Ceppi, Maurizio |
collection | PubMed |
description | BACKGROUND: Dendritic cells (DCs) are the sentinels of the mammalian immune system, characterized by a complex maturation process driven by pathogen detection. Although multiple studies have described the analysis of activated DCs by transcriptional profiling, recent findings indicate that mRNAs are also regulated at the translational level. A systematic analysis of the mRNAs being translationally regulated at various stages of DC activation was performed using translational profiling, which combines sucrose gradient fractionation of polysomal-bound mRNAs with DNA microarray analysis. RESULTS: Total and polysomal-bound mRNA populations purified from immature, 4 h and 16 h LPS-stimulated human monocyte-derived DCs were analyzed on Affymetrix microarrays U133 2.0. A group of 375 transcripts was identified as translationally regulated during DC-activation. In addition to several biochemical pathways related to immunity, the most statistically relevant biological function identified among the translationally regulated mRNAs was protein biosynthesis itself. We singled-out a cluster of 11 large ribosome proteins mRNAs, which are disengaged from polysomes at late time of maturation, suggesting the existence of a negative feedback loop regulating translation in DCs and linking ribosomal proteins to immuno-modulatory function. CONCLUSION: Our observations highlight the importance of translation regulation during the immune response, and may favor the identification of novel protein networks relevant for immunity. Our study also provides information on the potential absence of correlation between gene expression and protein production for specific mRNA molecules present in DCs. |
format | Text |
id | pubmed-2788525 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-27885252009-12-04 Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS Ceppi, Maurizio Clavarino, Giovanna Gatti, Evelina Schmidt, Enrico K de Gassart, Aude Blankenship, Derek Ogola, Gerald Banchereau, Jacques Chaussabel, Damien Pierre, Philippe Immunome Res Research BACKGROUND: Dendritic cells (DCs) are the sentinels of the mammalian immune system, characterized by a complex maturation process driven by pathogen detection. Although multiple studies have described the analysis of activated DCs by transcriptional profiling, recent findings indicate that mRNAs are also regulated at the translational level. A systematic analysis of the mRNAs being translationally regulated at various stages of DC activation was performed using translational profiling, which combines sucrose gradient fractionation of polysomal-bound mRNAs with DNA microarray analysis. RESULTS: Total and polysomal-bound mRNA populations purified from immature, 4 h and 16 h LPS-stimulated human monocyte-derived DCs were analyzed on Affymetrix microarrays U133 2.0. A group of 375 transcripts was identified as translationally regulated during DC-activation. In addition to several biochemical pathways related to immunity, the most statistically relevant biological function identified among the translationally regulated mRNAs was protein biosynthesis itself. We singled-out a cluster of 11 large ribosome proteins mRNAs, which are disengaged from polysomes at late time of maturation, suggesting the existence of a negative feedback loop regulating translation in DCs and linking ribosomal proteins to immuno-modulatory function. CONCLUSION: Our observations highlight the importance of translation regulation during the immune response, and may favor the identification of novel protein networks relevant for immunity. Our study also provides information on the potential absence of correlation between gene expression and protein production for specific mRNA molecules present in DCs. BioMed Central 2009-11-27 /pmc/articles/PMC2788525/ /pubmed/19943945 http://dx.doi.org/10.1186/1745-7580-5-5 Text en Copyright ©2009 Ceppi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Ceppi, Maurizio Clavarino, Giovanna Gatti, Evelina Schmidt, Enrico K de Gassart, Aude Blankenship, Derek Ogola, Gerald Banchereau, Jacques Chaussabel, Damien Pierre, Philippe Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS |
title | Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS |
title_full | Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS |
title_fullStr | Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS |
title_full_unstemmed | Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS |
title_short | Ribosomal protein mRNAs are translationally-regulated during human dendritic cells activation by LPS |
title_sort | ribosomal protein mrnas are translationally-regulated during human dendritic cells activation by lps |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2788525/ https://www.ncbi.nlm.nih.gov/pubmed/19943945 http://dx.doi.org/10.1186/1745-7580-5-5 |
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