Cargando…

An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe

Histone variant H2A.Z has a conserved role in genome stability, although it remains unclear how this is mediated. Here we demonstrate in fission yeast that the Swr1 ATPase inserts H2A.Z (Pht1) into chromatin and Kat5 acetyltransferase (Mst1) acetylates it. Deletion or unacetylatable mutation of Pht1...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Hyun-Soo, Vanoosthuyse, Vincent, Fillingham, Jeffrey, Roguev, Assen, Watt, Stephen, Kislinger, Thomas, Treyer, Alex, Carpenter, Laura Rocco, Bennett, Christopher S., Emili, Andrew, Greenblatt, Jack F., Hardwick, Kevin G., Krogan, Nevan J., Bähler, Jürg, Keogh, Michael-Christopher
Formato: Texto
Lenguaje:English
Publicado: 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2788674/
https://www.ncbi.nlm.nih.gov/pubmed/19915592
http://dx.doi.org/10.1038/nsmb.1688
_version_ 1782175007243239424
author Kim, Hyun-Soo
Vanoosthuyse, Vincent
Fillingham, Jeffrey
Roguev, Assen
Watt, Stephen
Kislinger, Thomas
Treyer, Alex
Carpenter, Laura Rocco
Bennett, Christopher S.
Emili, Andrew
Greenblatt, Jack F.
Hardwick, Kevin G.
Krogan, Nevan J.
Bähler, Jürg
Keogh, Michael-Christopher
author_facet Kim, Hyun-Soo
Vanoosthuyse, Vincent
Fillingham, Jeffrey
Roguev, Assen
Watt, Stephen
Kislinger, Thomas
Treyer, Alex
Carpenter, Laura Rocco
Bennett, Christopher S.
Emili, Andrew
Greenblatt, Jack F.
Hardwick, Kevin G.
Krogan, Nevan J.
Bähler, Jürg
Keogh, Michael-Christopher
author_sort Kim, Hyun-Soo
collection PubMed
description Histone variant H2A.Z has a conserved role in genome stability, although it remains unclear how this is mediated. Here we demonstrate in fission yeast that the Swr1 ATPase inserts H2A.Z (Pht1) into chromatin and Kat5 acetyltransferase (Mst1) acetylates it. Deletion or unacetylatable mutation of Pht1 leads to genome instability, primarily caused by chromosome entanglement/breakage at anaphase. This leads to the loss of telomere-proximal markers, though telomere protection and repeat length are unaffected by the absence of Pht1. Strikingly the chromosome entanglement in pht1Δ anaphase cells can be rescued by forcing chromosome condensation prior to anaphase onset. We show that the condensin complex, required for the maintenance of anaphase chromosome condensation, prematurely dissociates from chromatin in the absence of Pht1. This and other findings suggest an important role for H2A.Z in the architecture of anaphase chromosomes.
format Text
id pubmed-2788674
institution National Center for Biotechnology Information
language English
publishDate 2009
record_format MEDLINE/PubMed
spelling pubmed-27886742010-06-01 An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe Kim, Hyun-Soo Vanoosthuyse, Vincent Fillingham, Jeffrey Roguev, Assen Watt, Stephen Kislinger, Thomas Treyer, Alex Carpenter, Laura Rocco Bennett, Christopher S. Emili, Andrew Greenblatt, Jack F. Hardwick, Kevin G. Krogan, Nevan J. Bähler, Jürg Keogh, Michael-Christopher Nat Struct Mol Biol Article Histone variant H2A.Z has a conserved role in genome stability, although it remains unclear how this is mediated. Here we demonstrate in fission yeast that the Swr1 ATPase inserts H2A.Z (Pht1) into chromatin and Kat5 acetyltransferase (Mst1) acetylates it. Deletion or unacetylatable mutation of Pht1 leads to genome instability, primarily caused by chromosome entanglement/breakage at anaphase. This leads to the loss of telomere-proximal markers, though telomere protection and repeat length are unaffected by the absence of Pht1. Strikingly the chromosome entanglement in pht1Δ anaphase cells can be rescued by forcing chromosome condensation prior to anaphase onset. We show that the condensin complex, required for the maintenance of anaphase chromosome condensation, prematurely dissociates from chromatin in the absence of Pht1. This and other findings suggest an important role for H2A.Z in the architecture of anaphase chromosomes. 2009-11-15 2009-12 /pmc/articles/PMC2788674/ /pubmed/19915592 http://dx.doi.org/10.1038/nsmb.1688 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Kim, Hyun-Soo
Vanoosthuyse, Vincent
Fillingham, Jeffrey
Roguev, Assen
Watt, Stephen
Kislinger, Thomas
Treyer, Alex
Carpenter, Laura Rocco
Bennett, Christopher S.
Emili, Andrew
Greenblatt, Jack F.
Hardwick, Kevin G.
Krogan, Nevan J.
Bähler, Jürg
Keogh, Michael-Christopher
An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe
title An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe
title_full An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe
title_fullStr An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe
title_full_unstemmed An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe
title_short An acetylated form of histone H2A.Z regulates chromosome architecture in Schizosaccharomyces pombe
title_sort acetylated form of histone h2a.z regulates chromosome architecture in schizosaccharomyces pombe
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2788674/
https://www.ncbi.nlm.nih.gov/pubmed/19915592
http://dx.doi.org/10.1038/nsmb.1688
work_keys_str_mv AT kimhyunsoo anacetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT vanoosthuysevincent anacetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT fillinghamjeffrey anacetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT roguevassen anacetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT wattstephen anacetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT kislingerthomas anacetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT treyeralex anacetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT carpenterlaurarocco anacetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT bennettchristophers anacetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT emiliandrew anacetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT greenblattjackf anacetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT hardwickkeving anacetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT krogannevanj anacetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT bahlerjurg anacetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT keoghmichaelchristopher anacetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT kimhyunsoo acetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT vanoosthuysevincent acetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT fillinghamjeffrey acetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT roguevassen acetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT wattstephen acetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT kislingerthomas acetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT treyeralex acetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT carpenterlaurarocco acetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT bennettchristophers acetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT emiliandrew acetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT greenblattjackf acetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT hardwickkeving acetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT krogannevanj acetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT bahlerjurg acetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe
AT keoghmichaelchristopher acetylatedformofhistoneh2azregulateschromosomearchitectureinschizosaccharomycespombe