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Development and validation of a cytochrome c-coupled assay for pteridine reductase 1 and dihydrofolate reductase
Activity of the pterin- and folate-salvaging enzymes pteridine reductase 1 (PTR1) and dihydrofolate reductase–thymidylate synthetase (DHFR-TS) is commonly measured as a decrease in absorbance at 340 nm, corresponding to oxidation of nicotinamide adenine dinucleotide phosphate (NADPH). Although this...
| Autores principales: | , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Academic Press
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2789237/ https://www.ncbi.nlm.nih.gov/pubmed/19748480 http://dx.doi.org/10.1016/j.ab.2009.09.003 |