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Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis
Cytokinesis represents the final stage of eukaryotic cell division during which the cytoplasm becomes partitioned between daughter cells. The process differs to some extent between animal and plant cells, but proteins of the syntaxin family mediate membrane fusion in the plane of cell division in di...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2789941/ https://www.ncbi.nlm.nih.gov/pubmed/19959995 http://dx.doi.org/10.1038/emboj.2009.363 |
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author | Boutté, Yohann Frescatada-Rosa, Márcia Men, Shuzhen Chow, Cheung-Ming Ebine, Kazuo Gustavsson, Anna Johansson, Lenore Ueda, Takashi Moore, Ian Jürgens, Gerd Grebe, Markus |
author_facet | Boutté, Yohann Frescatada-Rosa, Márcia Men, Shuzhen Chow, Cheung-Ming Ebine, Kazuo Gustavsson, Anna Johansson, Lenore Ueda, Takashi Moore, Ian Jürgens, Gerd Grebe, Markus |
author_sort | Boutté, Yohann |
collection | PubMed |
description | Cytokinesis represents the final stage of eukaryotic cell division during which the cytoplasm becomes partitioned between daughter cells. The process differs to some extent between animal and plant cells, but proteins of the syntaxin family mediate membrane fusion in the plane of cell division in diverse organisms. How syntaxin localization is kept in check remains elusive. Here, we report that localization of the Arabidopsis KNOLLE syntaxin in the plane of cell division is maintained by sterol-dependent endocytosis involving a clathrin- and DYNAMIN-RELATED PROTEIN1A-dependent mechanism. On genetic or pharmacological interference with endocytosis, KNOLLE mis-localizes to lateral plasma membranes after cell-plate fusion. Fluorescence-loss-in-photo-bleaching and fluorescence-recovery-after-photo-bleaching experiments reveal lateral diffusion of GFP-KNOLLE from the plane of division to lateral membranes. In an endocytosis-defective sterol biosynthesis mutant displaying lateral KNOLLE diffusion, KNOLLE secretory trafficking remains unaffected. Thus, restriction of lateral diffusion by endocytosis may serve to maintain specificity of syntaxin localization during late cytokinesis. |
format | Text |
id | pubmed-2789941 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-27899412009-12-14 Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis Boutté, Yohann Frescatada-Rosa, Márcia Men, Shuzhen Chow, Cheung-Ming Ebine, Kazuo Gustavsson, Anna Johansson, Lenore Ueda, Takashi Moore, Ian Jürgens, Gerd Grebe, Markus EMBO J Article Cytokinesis represents the final stage of eukaryotic cell division during which the cytoplasm becomes partitioned between daughter cells. The process differs to some extent between animal and plant cells, but proteins of the syntaxin family mediate membrane fusion in the plane of cell division in diverse organisms. How syntaxin localization is kept in check remains elusive. Here, we report that localization of the Arabidopsis KNOLLE syntaxin in the plane of cell division is maintained by sterol-dependent endocytosis involving a clathrin- and DYNAMIN-RELATED PROTEIN1A-dependent mechanism. On genetic or pharmacological interference with endocytosis, KNOLLE mis-localizes to lateral plasma membranes after cell-plate fusion. Fluorescence-loss-in-photo-bleaching and fluorescence-recovery-after-photo-bleaching experiments reveal lateral diffusion of GFP-KNOLLE from the plane of division to lateral membranes. In an endocytosis-defective sterol biosynthesis mutant displaying lateral KNOLLE diffusion, KNOLLE secretory trafficking remains unaffected. Thus, restriction of lateral diffusion by endocytosis may serve to maintain specificity of syntaxin localization during late cytokinesis. Nature Publishing Group 2010-02-03 2009-12-03 /pmc/articles/PMC2789941/ /pubmed/19959995 http://dx.doi.org/10.1038/emboj.2009.363 Text en Copyright © 2010, European Molecular Biology Organization http://creativecommons.org/licenses/by-nc-nd/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits distribution, and reproduction in any medium, provided the original author and source are credited. This license does not permit commercial exploitation or the creation of derivative works without specific permission. |
spellingShingle | Article Boutté, Yohann Frescatada-Rosa, Márcia Men, Shuzhen Chow, Cheung-Ming Ebine, Kazuo Gustavsson, Anna Johansson, Lenore Ueda, Takashi Moore, Ian Jürgens, Gerd Grebe, Markus Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis |
title | Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis |
title_full | Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis |
title_fullStr | Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis |
title_full_unstemmed | Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis |
title_short | Endocytosis restricts Arabidopsis KNOLLE syntaxin to the cell division plane during late cytokinesis |
title_sort | endocytosis restricts arabidopsis knolle syntaxin to the cell division plane during late cytokinesis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2789941/ https://www.ncbi.nlm.nih.gov/pubmed/19959995 http://dx.doi.org/10.1038/emboj.2009.363 |
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