Cargando…

DNA cleavage and methylation specificity of the single polypeptide restriction–modification enzyme LlaGI

LlaGI is a single polypeptide restriction–modification enzyme encoded on the naturally-occurring plasmid pEW104 isolated from Lactococcus lactis ssp. cremoris W10. Bioinformatics analysis suggests that the enzyme contains domains characteristic of an mrr endonuclease, a superfamily 2 DNA helicase an...

Descripción completa

Detalles Bibliográficos
Autores principales:	Smith, Rachel M., Diffin, Fiona M., Savery, Nigel J., Josephsen, Jytte, Szczelkun, Mark D.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2009
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2790903/ https://www.ncbi.nlm.nih.gov/pubmed/19808936 http://dx.doi.org/10.1093/nar/gkp790

Ejemplares similares

An Mrr-family nuclease motif in the single polypeptide restriction–modification enzyme LlaGI
por: Smith, Rachel M., et al.
Publicado: (2009)

The single polypeptide restriction–modification enzyme LlaGI is a self-contained molecular motor that translocates DNA loops
por: Smith, Rachel M., et al.
Publicado: (2009)

The Type ISP Restriction–Modification enzymes LlaBIII and LlaGI use a translocation–collision mechanism to cleave non-specific DNA distant from their recognition sites
por: Šišáková, Eva, et al.
Publicado: (2013)

Recycling of protein subunits during DNA translocation and cleavage by Type I restriction-modification enzymes
por: Simons, Michelle, et al.
Publicado: (2011)

DNA cleavage by Type ISP Restriction–Modification enzymes is initially targeted to the 3′-5′ strand
por: van Aelst, Kara, et al.
Publicado: (2013)

ClpXP protease targets long-lived DNA translocation states of a helicase-like motor to cause restriction alleviation
por: Simons, Michelle, et al.
Publicado: (2014)

5′ modifications to CRISPR–Cas9 gRNA can change the dynamics and size of R-loops and inhibit DNA cleavage
por: Mullally, Grace, et al.
Publicado: (2020)

Mapping DNA cleavage by the Type ISP restriction-modification enzymes following long-range communication between DNA sites in different orientations
por: van Aelst, Kara, et al.
Publicado: (2015)

TstI, a Type II restriction–modification protein with DNA recognition, cleavage and methylation functions in a single polypeptide
por: Smith, Rachel M., et al.
Publicado: (2014)

DNA base flipping by both members of the PspGI restriction–modification system
por: Carpenter, Michael A., et al.
Publicado: (2008)

Controlling the motor activity of a transcription-repair coupling factor: autoinhibition and the role of RNA polymerase
por: Smith, Abigail J., et al.
Publicado: (2007)

DNA cleavage site selection by Type III restriction enzymes provides evidence for head-on protein collisions following 1D bidirectional motion
por: Schwarz, Friedrich W., et al.
Publicado: (2011)

Dissociation from DNA of Type III Restriction–Modification enzymes during helicase-dependent motion and following endonuclease activity
por: Tóth, Júlia, et al.
Publicado: (2012)

Length heterogeneity at conserved sequence block 2 in human mitochondrial DNA acts as a rheostat for RNA polymerase POLRMT activity
por: Tan, Benedict G., et al.
Publicado: (2016)

An investigation of the structural requirements for ATP hydrolysis and DNA cleavage by the EcoKI Type I DNA restriction and modification enzyme
por: Roberts, Gareth A., et al.
Publicado: (2011)

Organization of the BcgI restriction-modification protein for the cleavage of eight phosphodiester bonds in DNA
por: Smith, Rachel M., et al.
Publicado: (2013)

Structure and cleavage activity of the tetrameric MspJI DNA modification-dependent restriction endonuclease
por: Horton, John R., et al.
Publicado: (2012)

Restriction-modification system with methyl-inhibited base excision and abasic-site cleavage activities
por: Fukuyo, Masaki, et al.
Publicado: (2015)

Sequence-specific cleavage of RNA by Type II restriction enzymes
por: Murray, Iain A., et al.
Publicado: (2010)

Re-evaluating the kinetics of ATP hydrolysis during initiation of DNA sliding by Type III restriction enzymes
por: Tóth, Júlia, et al.
Publicado: (2015)

S-Adenosyl homocysteine and DNA ends stimulate promiscuous nuclease activities in the Type III restriction endonuclease EcoPI
por: Peakman, Luke J., et al.
Publicado: (2009)

Increasing cleavage specificity and activity of restriction endonuclease KpnI
por: Vasu, Kommireddy, et al.
Publicado: (2013)

DNA synapsis through transient tetramerization triggers cleavage by Ecl18kI restriction enzyme
por: Zaremba, Mindaugas, et al.
Publicado: (2010)

A model for the evolution of prokaryotic DNA restriction-modification systems based upon the structural malleability of Type I restriction-modification enzymes
por: Bower, Edward K M, et al.
Publicado: (2018)

DNA cleavage by CgII and NgoAVII requires interaction between N- and R-proteins and extensive nucleotide hydrolysis
por: Zaremba, Mindaugas, et al.
Publicado: (2014)

A RecB-family nuclease motif in the Type I restriction endonuclease EcoR124I
por: Šišáková, Eva, et al.
Publicado: (2008)

Cleavage of adenine-modified functionalized DNA by type II restriction endonucleases
por: Macíčková-Cahová, Hana, et al.
Publicado: (2009)

CgII cleaves DNA using a mechanism distinct from other ATP-dependent restriction endonucleases
por: Toliusis, Paulius, et al.
Publicado: (2017)

The MmeI family: type II restriction–modification enzymes that employ single-strand modification for host protection
por: Morgan, Richard D., et al.
Publicado: (2009)

Rational engineering of type II restriction endonuclease DNA binding and cleavage specificity
por: Morgan, Richard D., et al.
Publicado: (2009)

Target site cleavage by the monomeric restriction enzyme BcnI requires translocation to a random DNA sequence and a switch in enzyme orientation
por: Sasnauskas, Giedrius, et al.
Publicado: (2011)

Single-site DNA cleavage by Type III restriction endonuclease requires a site-bound enzyme and a trans-acting enzyme that are ATPase-activated
por: Ahmad, Ishtiyaq, et al.
Publicado: (2018)

BspRI restriction endonuclease: cloning, expression in Escherichia coli and sequential cleavage mechanism
por: Raskó, Tamás, et al.
Publicado: (2010)

Type III restriction endonucleases are heterotrimeric: comprising one helicase–nuclease subunit and a dimeric methyltransferase that binds only one specific DNA
por: Butterer, Annika, et al.
Publicado: (2014)

Novel m4C modification in type I restriction-modification systems
por: Morgan, Richard D., et al.
Publicado: (2016)

Multipartite control of the DNA translocase, Mfd
por: Smith, Abigail J., et al.
Publicado: (2012)

DNA-mediated coupling of ATPase, translocase and nuclease activities of a Type ISP restriction-modification enzyme
por: Chand, Mahesh Kumar, et al.
Publicado: (2020)

Catalytic domain of restriction endonuclease BmrI as a cleavage module for engineering endonucleases with novel substrate specificities
por: Chan, Siu-hong, et al.
Publicado: (2007)

The H-subunit of the restriction endonuclease CglI contains a prototype DEAD-Z1 helicase-like motor
por: Toliusis, Paulius, et al.
Publicado: (2018)

Antisense RNA associated with biological regulation of a restriction–modification system
por: Mruk, Iwona, et al.
Publicado: (2011)

Cannot write session to /tmp/vufind_sessions/sess_eijmpd40ho1hj2jbmj94vfq694