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The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases

Sunburn is a commonly occurring acute inflammatory process, with dermal vasodilatation and leukocyte infiltration as central features. Ultraviolet (UV) B-induced hydrolysis of membrane phospholipids releases polyunsaturated fatty acids, and their subsequent metabolism by cyclooxygenases (COXs) and l...

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Autores principales: Rhodes, Lesley E., Gledhill, Karl, Masoodi, Mojgan, Haylett, Ann K., Brownrigg, Margaret, Thody, Anthony J., Tobin, Desmond J., Nicolaou, Anna
Formato: Texto
Lenguaje:English
Publicado: The Federation of American Societies for Experimental Biology 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2791058/
https://www.ncbi.nlm.nih.gov/pubmed/19584301
http://dx.doi.org/10.1096/fj.09-136077
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author Rhodes, Lesley E.
Gledhill, Karl
Masoodi, Mojgan
Haylett, Ann K.
Brownrigg, Margaret
Thody, Anthony J.
Tobin, Desmond J.
Nicolaou, Anna
author_facet Rhodes, Lesley E.
Gledhill, Karl
Masoodi, Mojgan
Haylett, Ann K.
Brownrigg, Margaret
Thody, Anthony J.
Tobin, Desmond J.
Nicolaou, Anna
author_sort Rhodes, Lesley E.
collection PubMed
description Sunburn is a commonly occurring acute inflammatory process, with dermal vasodilatation and leukocyte infiltration as central features. Ultraviolet (UV) B-induced hydrolysis of membrane phospholipids releases polyunsaturated fatty acids, and their subsequent metabolism by cyclooxygenases (COXs) and lipoxygenases (LOXs) may produce potent eicosanoid mediators modulating different stages of the inflammation. Our objective was to identify candidate eicosanoids formed during the sunburn reaction in relation to its clinical and histological course. We exposed skin of healthy humans (n=32) to UVB and, for 72 h, examined expression of proinflammatory and anti-inflammatory eicosanoids using LC/ESI-MS/MS, and examined immunohistochemical expression of COX-2, 12-LOX, 15-LOX, and leukocyte markers, while quantifying clinical erythema. We show that vasodilatory prostaglandins (PGs) PGE(2), PGF(2α), and PGE(3) accompany the erythema in the first 24–48 h, associated with increased COX-2 expression at 24 h. Novel, potent leukocyte chemoattractants 11-, 12-, and 8-monohydroxy-eicosatetraenoic acid (HETE) are elevated from 4 to 72 h, in association with peak dermal neutrophil influx at 24 h, and increased dermal CD3(+) lymphocytes and 12- and 15-LOX expression from 24 to 72 h. Anti-inflammatory metabolite 15-HETE shows later expression, peaking at 72 h. Sunburn is characterized by overlapping sequential profiles of increases in COX products followed by LOX products that may regulate subsequent events and ultimately its resolution.—Rhodes, L. E., Gledhill, K., Masoodi, M., Haylett, A. K., Brownrigg, M., Thody, A. J., Tobin, D. J., Nicolaou, A. The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases.
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spelling pubmed-27910582010-01-28 The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases Rhodes, Lesley E. Gledhill, Karl Masoodi, Mojgan Haylett, Ann K. Brownrigg, Margaret Thody, Anthony J. Tobin, Desmond J. Nicolaou, Anna FASEB J Research Communications Sunburn is a commonly occurring acute inflammatory process, with dermal vasodilatation and leukocyte infiltration as central features. Ultraviolet (UV) B-induced hydrolysis of membrane phospholipids releases polyunsaturated fatty acids, and their subsequent metabolism by cyclooxygenases (COXs) and lipoxygenases (LOXs) may produce potent eicosanoid mediators modulating different stages of the inflammation. Our objective was to identify candidate eicosanoids formed during the sunburn reaction in relation to its clinical and histological course. We exposed skin of healthy humans (n=32) to UVB and, for 72 h, examined expression of proinflammatory and anti-inflammatory eicosanoids using LC/ESI-MS/MS, and examined immunohistochemical expression of COX-2, 12-LOX, 15-LOX, and leukocyte markers, while quantifying clinical erythema. We show that vasodilatory prostaglandins (PGs) PGE(2), PGF(2α), and PGE(3) accompany the erythema in the first 24–48 h, associated with increased COX-2 expression at 24 h. Novel, potent leukocyte chemoattractants 11-, 12-, and 8-monohydroxy-eicosatetraenoic acid (HETE) are elevated from 4 to 72 h, in association with peak dermal neutrophil influx at 24 h, and increased dermal CD3(+) lymphocytes and 12- and 15-LOX expression from 24 to 72 h. Anti-inflammatory metabolite 15-HETE shows later expression, peaking at 72 h. Sunburn is characterized by overlapping sequential profiles of increases in COX products followed by LOX products that may regulate subsequent events and ultimately its resolution.—Rhodes, L. E., Gledhill, K., Masoodi, M., Haylett, A. K., Brownrigg, M., Thody, A. J., Tobin, D. J., Nicolaou, A. The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases. The Federation of American Societies for Experimental Biology 2009-11 /pmc/articles/PMC2791058/ /pubmed/19584301 http://dx.doi.org/10.1096/fj.09-136077 Text en © 2009 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/us/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Communications
Rhodes, Lesley E.
Gledhill, Karl
Masoodi, Mojgan
Haylett, Ann K.
Brownrigg, Margaret
Thody, Anthony J.
Tobin, Desmond J.
Nicolaou, Anna
The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases
title The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases
title_full The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases
title_fullStr The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases
title_full_unstemmed The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases
title_short The sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases
title_sort sunburn response in human skin is characterized by sequential eicosanoid profiles that may mediate its early and late phases
topic Research Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2791058/
https://www.ncbi.nlm.nih.gov/pubmed/19584301
http://dx.doi.org/10.1096/fj.09-136077
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