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Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit

Loquat (Eriobotrya japonica Lindl.) is a subtropical fruit, with some cultivars such as ‘Luoyangqing’ (LYQ) susceptible to chilling injury (CI), while others such as ‘Baisha’ (BS) are resistant. Although loquats are non-climacteric, modulation of ethylene has an effect on ripening-related post-harve...

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Autores principales: Wang, Ping, Zhang, Bo, Li, Xian, Xu, Changjie, Yin, Xueren, Shan, Lanlan, Ferguson, Ian, Chen, Kunsong
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2791125/
https://www.ncbi.nlm.nih.gov/pubmed/19884229
http://dx.doi.org/10.1093/jxb/erp302
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author Wang, Ping
Zhang, Bo
Li, Xian
Xu, Changjie
Yin, Xueren
Shan, Lanlan
Ferguson, Ian
Chen, Kunsong
author_facet Wang, Ping
Zhang, Bo
Li, Xian
Xu, Changjie
Yin, Xueren
Shan, Lanlan
Ferguson, Ian
Chen, Kunsong
author_sort Wang, Ping
collection PubMed
description Loquat (Eriobotrya japonica Lindl.) is a subtropical fruit, with some cultivars such as ‘Luoyangqing’ (LYQ) susceptible to chilling injury (CI), while others such as ‘Baisha’ (BS) are resistant. Although loquats are non-climacteric, modulation of ethylene has an effect on ripening-related post-harvest CI. Therefore the role of ethylene signalling in the development of CI was investigated in fruit of both the LYQ and BS cultivars. Three ethylene receptor genes, one CTR1-like gene, and one EIN3-like gene were isolated and characterized in ripening fruit. All of these genes were expressed differentially within and between fruit of the two cultivars. Transcripts either declined over fruit development (EjERS1a in both cultivars and EjEIL1 in LYQ) or showed an increase in the middle stages of fruit development before declining (EjETR1, EjERS1b, and EjCTR1 in both cultivars and EjEIL1 in BS). The main cultivar differences were in levels rather than in patterns of expression during post-harvest storage. EjETR1, EjCTR1, and EjEIL1 genes showed increased expression in response to low temperature and this was particularly notable for EjETR1, and EjEIL1 during CI development in LYQ fruit. The genes were also differentially responsive to ethylene treatment, 1-methycyclopropene (1-MCP) and low temperature conditioning, confirming a role for ethylene in regulation of CI in loquat fruit.
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spelling pubmed-27911252009-12-10 Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit Wang, Ping Zhang, Bo Li, Xian Xu, Changjie Yin, Xueren Shan, Lanlan Ferguson, Ian Chen, Kunsong J Exp Bot Research Papers Loquat (Eriobotrya japonica Lindl.) is a subtropical fruit, with some cultivars such as ‘Luoyangqing’ (LYQ) susceptible to chilling injury (CI), while others such as ‘Baisha’ (BS) are resistant. Although loquats are non-climacteric, modulation of ethylene has an effect on ripening-related post-harvest CI. Therefore the role of ethylene signalling in the development of CI was investigated in fruit of both the LYQ and BS cultivars. Three ethylene receptor genes, one CTR1-like gene, and one EIN3-like gene were isolated and characterized in ripening fruit. All of these genes were expressed differentially within and between fruit of the two cultivars. Transcripts either declined over fruit development (EjERS1a in both cultivars and EjEIL1 in LYQ) or showed an increase in the middle stages of fruit development before declining (EjETR1, EjERS1b, and EjCTR1 in both cultivars and EjEIL1 in BS). The main cultivar differences were in levels rather than in patterns of expression during post-harvest storage. EjETR1, EjCTR1, and EjEIL1 genes showed increased expression in response to low temperature and this was particularly notable for EjETR1, and EjEIL1 during CI development in LYQ fruit. The genes were also differentially responsive to ethylene treatment, 1-methycyclopropene (1-MCP) and low temperature conditioning, confirming a role for ethylene in regulation of CI in loquat fruit. Oxford University Press 2010-01 2009-10-30 /pmc/articles/PMC2791125/ /pubmed/19884229 http://dx.doi.org/10.1093/jxb/erp302 Text en © 2009 The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)
spellingShingle Research Papers
Wang, Ping
Zhang, Bo
Li, Xian
Xu, Changjie
Yin, Xueren
Shan, Lanlan
Ferguson, Ian
Chen, Kunsong
Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit
title Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit
title_full Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit
title_fullStr Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit
title_full_unstemmed Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit
title_short Ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit
title_sort ethylene signal transduction elements involved in chilling injury in non-climacteric loquat fruit
topic Research Papers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2791125/
https://www.ncbi.nlm.nih.gov/pubmed/19884229
http://dx.doi.org/10.1093/jxb/erp302
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