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In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE)

Melanoma antigen family (MAGE) genes are widely expressed in various tumor types but silent in normal cells except germ-line cells lacking human leukocyte antigen (HLA) expression. Over 25 MAGE genes have been identified in different tissues, mostly located in Xq28 of human chromosome and some of th...

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Detalles Bibliográficos
Autores principales: Amin, Ruhul, Jesmin, Jamil, Hasan, Hossain, M. Anwar
Formato: Texto
Lenguaje:English
Publicado: Libertas Academica 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2791492/
https://www.ncbi.nlm.nih.gov/pubmed/20011463
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author Amin, Ruhul
Jesmin,
Jamil, Hasan
Hossain, M. Anwar
author_facet Amin, Ruhul
Jesmin,
Jamil, Hasan
Hossain, M. Anwar
author_sort Amin, Ruhul
collection PubMed
description Melanoma antigen family (MAGE) genes are widely expressed in various tumor types but silent in normal cells except germ-line cells lacking human leukocyte antigen (HLA) expression. Over 25 MAGE genes have been identified in different tissues, mostly located in Xq28 of human chromosome and some of them in chromosome 3 and 15, containing either single or multiple-exons. This in silico study predicted the genes on hTERT location and identified a distant relative of MAGE gene located on chromosome 5. The study identified a single exon coding ~850 residues polypeptide sharing ~30% homology with Macfa-MAGE E1 and hMAGE-E1. dbEST search of the predicted transcript matches 5′ and 3′ flanking ESTs. The predicted protein showed sequence homology within the MAGE homology domain 2 (MHD2). UCSC genome annotation of CpG Island around the coding region reveals that this gene could be silent by methylation. Affymetrix all-exon track indicates the gene could be expressed in different tissues particularly in cancer cells as they widely undergo a genome wide demethylation process.
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spelling pubmed-27914922009-12-11 In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE) Amin, Ruhul Jesmin, Jamil, Hasan Hossain, M. Anwar Cancer Inform Hypothesis Melanoma antigen family (MAGE) genes are widely expressed in various tumor types but silent in normal cells except germ-line cells lacking human leukocyte antigen (HLA) expression. Over 25 MAGE genes have been identified in different tissues, mostly located in Xq28 of human chromosome and some of them in chromosome 3 and 15, containing either single or multiple-exons. This in silico study predicted the genes on hTERT location and identified a distant relative of MAGE gene located on chromosome 5. The study identified a single exon coding ~850 residues polypeptide sharing ~30% homology with Macfa-MAGE E1 and hMAGE-E1. dbEST search of the predicted transcript matches 5′ and 3′ flanking ESTs. The predicted protein showed sequence homology within the MAGE homology domain 2 (MHD2). UCSC genome annotation of CpG Island around the coding region reveals that this gene could be silent by methylation. Affymetrix all-exon track indicates the gene could be expressed in different tissues particularly in cancer cells as they widely undergo a genome wide demethylation process. Libertas Academica 2009-11-24 /pmc/articles/PMC2791492/ /pubmed/20011463 Text en © 2009 The authors. http://creativecommons.org/licenses/by/2.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/2.0/).
spellingShingle Hypothesis
Amin, Ruhul
Jesmin,
Jamil, Hasan
Hossain, M. Anwar
In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE)
title In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE)
title_full In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE)
title_fullStr In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE)
title_full_unstemmed In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE)
title_short In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE)
title_sort in silico analysis of human telomerase reverse transcriptase (htert) gene: identification of a distant homolog of melanoma antigen family gene (mage)
topic Hypothesis
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2791492/
https://www.ncbi.nlm.nih.gov/pubmed/20011463
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