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In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE)
Melanoma antigen family (MAGE) genes are widely expressed in various tumor types but silent in normal cells except germ-line cells lacking human leukocyte antigen (HLA) expression. Over 25 MAGE genes have been identified in different tissues, mostly located in Xq28 of human chromosome and some of th...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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Libertas Academica
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2791492/ https://www.ncbi.nlm.nih.gov/pubmed/20011463 |
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author | Amin, Ruhul Jesmin, Jamil, Hasan Hossain, M. Anwar |
author_facet | Amin, Ruhul Jesmin, Jamil, Hasan Hossain, M. Anwar |
author_sort | Amin, Ruhul |
collection | PubMed |
description | Melanoma antigen family (MAGE) genes are widely expressed in various tumor types but silent in normal cells except germ-line cells lacking human leukocyte antigen (HLA) expression. Over 25 MAGE genes have been identified in different tissues, mostly located in Xq28 of human chromosome and some of them in chromosome 3 and 15, containing either single or multiple-exons. This in silico study predicted the genes on hTERT location and identified a distant relative of MAGE gene located on chromosome 5. The study identified a single exon coding ~850 residues polypeptide sharing ~30% homology with Macfa-MAGE E1 and hMAGE-E1. dbEST search of the predicted transcript matches 5′ and 3′ flanking ESTs. The predicted protein showed sequence homology within the MAGE homology domain 2 (MHD2). UCSC genome annotation of CpG Island around the coding region reveals that this gene could be silent by methylation. Affymetrix all-exon track indicates the gene could be expressed in different tissues particularly in cancer cells as they widely undergo a genome wide demethylation process. |
format | Text |
id | pubmed-2791492 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Libertas Academica |
record_format | MEDLINE/PubMed |
spelling | pubmed-27914922009-12-11 In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE) Amin, Ruhul Jesmin, Jamil, Hasan Hossain, M. Anwar Cancer Inform Hypothesis Melanoma antigen family (MAGE) genes are widely expressed in various tumor types but silent in normal cells except germ-line cells lacking human leukocyte antigen (HLA) expression. Over 25 MAGE genes have been identified in different tissues, mostly located in Xq28 of human chromosome and some of them in chromosome 3 and 15, containing either single or multiple-exons. This in silico study predicted the genes on hTERT location and identified a distant relative of MAGE gene located on chromosome 5. The study identified a single exon coding ~850 residues polypeptide sharing ~30% homology with Macfa-MAGE E1 and hMAGE-E1. dbEST search of the predicted transcript matches 5′ and 3′ flanking ESTs. The predicted protein showed sequence homology within the MAGE homology domain 2 (MHD2). UCSC genome annotation of CpG Island around the coding region reveals that this gene could be silent by methylation. Affymetrix all-exon track indicates the gene could be expressed in different tissues particularly in cancer cells as they widely undergo a genome wide demethylation process. Libertas Academica 2009-11-24 /pmc/articles/PMC2791492/ /pubmed/20011463 Text en © 2009 The authors. http://creativecommons.org/licenses/by/2.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/2.0/). |
spellingShingle | Hypothesis Amin, Ruhul Jesmin, Jamil, Hasan Hossain, M. Anwar In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE) |
title | In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE) |
title_full | In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE) |
title_fullStr | In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE) |
title_full_unstemmed | In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE) |
title_short | In silico Analysis of Human Telomerase Reverse Transcriptase (hTERT) Gene: Identification of a Distant Homolog of Melanoma Antigen Family Gene (MAGE) |
title_sort | in silico analysis of human telomerase reverse transcriptase (htert) gene: identification of a distant homolog of melanoma antigen family gene (mage) |
topic | Hypothesis |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2791492/ https://www.ncbi.nlm.nih.gov/pubmed/20011463 |
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