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Salmonella Induced IL-23 and IL-1β Allow for IL-12 Production by Monocytes and Mϕ1 through Induction of IFN-γ in CD56(+) NK/NK-Like T Cells

BACKGROUND: The type-1 cytokine pathway plays a pivotal role in immunity against intracellular bacterial pathogens such as Salmonellae and Mycobacteria. Bacterial stimulation of pattern recognition receptors on monocytes, macrophages and dendritic cells initiates this pathway, and results in the pro...

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Autores principales: van de Wetering, Diederik, de Paus, Roelof A., van Dissel, Jaap T., van de Vosse, Esther
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2791865/
https://www.ncbi.nlm.nih.gov/pubmed/20027291
http://dx.doi.org/10.1371/journal.pone.0008396
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author van de Wetering, Diederik
de Paus, Roelof A.
van Dissel, Jaap T.
van de Vosse, Esther
author_facet van de Wetering, Diederik
de Paus, Roelof A.
van Dissel, Jaap T.
van de Vosse, Esther
author_sort van de Wetering, Diederik
collection PubMed
description BACKGROUND: The type-1 cytokine pathway plays a pivotal role in immunity against intracellular bacterial pathogens such as Salmonellae and Mycobacteria. Bacterial stimulation of pattern recognition receptors on monocytes, macrophages and dendritic cells initiates this pathway, and results in the production of cytokines that activate lymphocytes to produce interferon (IFN)-γ. Interleukin (IL)-12 and IL-23 are thought to be the key cytokines required for initiating a type-1 cytokine immune response to Mycobacteria and Salmonellae. The relative contribution of IL-23 and IL-12 to this process is uncertain. METHODOLOGY/PRINCIPAL FINDINGS: We show that various TLR agonists induce the production of IL-23 but not IL-12 in freshly isolated human monocytes and cultured human macrophages. In addition, type 1 pro-inflammatory macrophages (Mϕ1) differentiated in the presence of GM-CSF and infected with live Salmonella produce IL-23, IL-1β and IL-18, but not IL-12. Supernatants of Salmonella-infected Mϕ1 contained more IL-18 and IL-1β as compared with supernatants of Mϕ1 stimulated with isolated TLR agonists, and induced IFN-γ production in human CD56(+) cells in an IL-23 and IL-1β-dependent but IL-12-independent manner. In addition, IL-23 together with IL-18 or IL-1β led to the production of GM-CSF in CD56(+) cells. Both IFN-γ and GM-CSF enhanced IL-23 production by monocytes in response to TLR agonists, as well as induced IL-12 production. CONCLUSIONS/SIGNIFICANCE: The findings implicate a positive feedback loop in which IL-23 can enhance its release via induction of IFN-γ and GM-CSF. The IL-23 induced cytokines allow for the subsequent production of IL-12 and amplify the IFN-γ production in the type-1 cytokine pathway.
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spelling pubmed-27918652009-12-22 Salmonella Induced IL-23 and IL-1β Allow for IL-12 Production by Monocytes and Mϕ1 through Induction of IFN-γ in CD56(+) NK/NK-Like T Cells van de Wetering, Diederik de Paus, Roelof A. van Dissel, Jaap T. van de Vosse, Esther PLoS One Research Article BACKGROUND: The type-1 cytokine pathway plays a pivotal role in immunity against intracellular bacterial pathogens such as Salmonellae and Mycobacteria. Bacterial stimulation of pattern recognition receptors on monocytes, macrophages and dendritic cells initiates this pathway, and results in the production of cytokines that activate lymphocytes to produce interferon (IFN)-γ. Interleukin (IL)-12 and IL-23 are thought to be the key cytokines required for initiating a type-1 cytokine immune response to Mycobacteria and Salmonellae. The relative contribution of IL-23 and IL-12 to this process is uncertain. METHODOLOGY/PRINCIPAL FINDINGS: We show that various TLR agonists induce the production of IL-23 but not IL-12 in freshly isolated human monocytes and cultured human macrophages. In addition, type 1 pro-inflammatory macrophages (Mϕ1) differentiated in the presence of GM-CSF and infected with live Salmonella produce IL-23, IL-1β and IL-18, but not IL-12. Supernatants of Salmonella-infected Mϕ1 contained more IL-18 and IL-1β as compared with supernatants of Mϕ1 stimulated with isolated TLR agonists, and induced IFN-γ production in human CD56(+) cells in an IL-23 and IL-1β-dependent but IL-12-independent manner. In addition, IL-23 together with IL-18 or IL-1β led to the production of GM-CSF in CD56(+) cells. Both IFN-γ and GM-CSF enhanced IL-23 production by monocytes in response to TLR agonists, as well as induced IL-12 production. CONCLUSIONS/SIGNIFICANCE: The findings implicate a positive feedback loop in which IL-23 can enhance its release via induction of IFN-γ and GM-CSF. The IL-23 induced cytokines allow for the subsequent production of IL-12 and amplify the IFN-γ production in the type-1 cytokine pathway. Public Library of Science 2009-12-21 /pmc/articles/PMC2791865/ /pubmed/20027291 http://dx.doi.org/10.1371/journal.pone.0008396 Text en van de Wetering et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
van de Wetering, Diederik
de Paus, Roelof A.
van Dissel, Jaap T.
van de Vosse, Esther
Salmonella Induced IL-23 and IL-1β Allow for IL-12 Production by Monocytes and Mϕ1 through Induction of IFN-γ in CD56(+) NK/NK-Like T Cells
title Salmonella Induced IL-23 and IL-1β Allow for IL-12 Production by Monocytes and Mϕ1 through Induction of IFN-γ in CD56(+) NK/NK-Like T Cells
title_full Salmonella Induced IL-23 and IL-1β Allow for IL-12 Production by Monocytes and Mϕ1 through Induction of IFN-γ in CD56(+) NK/NK-Like T Cells
title_fullStr Salmonella Induced IL-23 and IL-1β Allow for IL-12 Production by Monocytes and Mϕ1 through Induction of IFN-γ in CD56(+) NK/NK-Like T Cells
title_full_unstemmed Salmonella Induced IL-23 and IL-1β Allow for IL-12 Production by Monocytes and Mϕ1 through Induction of IFN-γ in CD56(+) NK/NK-Like T Cells
title_short Salmonella Induced IL-23 and IL-1β Allow for IL-12 Production by Monocytes and Mϕ1 through Induction of IFN-γ in CD56(+) NK/NK-Like T Cells
title_sort salmonella induced il-23 and il-1β allow for il-12 production by monocytes and mϕ1 through induction of ifn-γ in cd56(+) nk/nk-like t cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2791865/
https://www.ncbi.nlm.nih.gov/pubmed/20027291
http://dx.doi.org/10.1371/journal.pone.0008396
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