Validation of a new method for immobilising kinetoplastid parasites for live cell imaging

The kinetoplastid parasites are responsible for three of the ten most neglected tropical diseases as classified by the WHO. Recent advances in molecular and cellular analyses have allowed rapid progress in our understanding of the biology of these lethal pathogens. In this study we validate a new me...

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Detalles Bibliográficos
Autores principales: Price, Helen P., MacLean, Lorna, Marrison, Joanne, O’Toole, Peter J., Smith, Deborah F.
Formato: Texto
Lenguaje:English
Publicado: Elsevier/North-Holland Biomedical Press 2010
Materias:
Short Technical Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2791879/
https://www.ncbi.nlm.nih.gov/pubmed/19815033
http://dx.doi.org/10.1016/j.molbiopara.2009.09.008
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author Price, Helen P.
MacLean, Lorna
Marrison, Joanne
O’Toole, Peter J.
Smith, Deborah F.
author_facet Price, Helen P.
MacLean, Lorna
Marrison, Joanne
O’Toole, Peter J.
Smith, Deborah F.
author_sort Price, Helen P.
collection PubMed
description The kinetoplastid parasites are responsible for three of the ten most neglected tropical diseases as classified by the WHO. Recent advances in molecular and cellular analyses have allowed rapid progress in our understanding of the biology of these lethal pathogens. In this study we validate a new method for immobilising Trypanosoma brucei and Leishmania major parasites while maintaining a high level of viability. This allows reproducible live cell imaging of these highly motile organisms, thus enabling a full complement of advanced microscopic techniques to be utilised to better understand these pathogenic species.
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spelling pubmed-27918792009-12-22 Validation of a new method for immobilising kinetoplastid parasites for live cell imaging Price, Helen P. MacLean, Lorna Marrison, Joanne O’Toole, Peter J. Smith, Deborah F. Mol Biochem Parasitol Short Technical Report The kinetoplastid parasites are responsible for three of the ten most neglected tropical diseases as classified by the WHO. Recent advances in molecular and cellular analyses have allowed rapid progress in our understanding of the biology of these lethal pathogens. In this study we validate a new method for immobilising Trypanosoma brucei and Leishmania major parasites while maintaining a high level of viability. This allows reproducible live cell imaging of these highly motile organisms, thus enabling a full complement of advanced microscopic techniques to be utilised to better understand these pathogenic species. Elsevier/North-Holland Biomedical Press 2010-01 /pmc/articles/PMC2791879/ /pubmed/19815033 http://dx.doi.org/10.1016/j.molbiopara.2009.09.008 Text en © 2010 Elsevier B.V. https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Short Technical Report
Price, Helen P.
MacLean, Lorna
Marrison, Joanne
O’Toole, Peter J.
Smith, Deborah F.
Validation of a new method for immobilising kinetoplastid parasites for live cell imaging
title Validation of a new method for immobilising kinetoplastid parasites for live cell imaging
title_full Validation of a new method for immobilising kinetoplastid parasites for live cell imaging
title_fullStr Validation of a new method for immobilising kinetoplastid parasites for live cell imaging
title_full_unstemmed Validation of a new method for immobilising kinetoplastid parasites for live cell imaging
title_short Validation of a new method for immobilising kinetoplastid parasites for live cell imaging
title_sort validation of a new method for immobilising kinetoplastid parasites for live cell imaging
topic Short Technical Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2791879/
https://www.ncbi.nlm.nih.gov/pubmed/19815033
http://dx.doi.org/10.1016/j.molbiopara.2009.09.008
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