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Effects of constitutively active GTPases on fibroblast behavior

The GTP-binding proteins RhoA, Cdc42 and Rac1 regulate the organization and turnover of the cytoskeleton and cell-matrix adhesions, structures bridging cells to their support, and translating forces, external or generated within the cell. To investigate the specific requirements of Rho GTPases for b...

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Autores principales: Zhang, Z.-G., Lambert, C. A., Servotte, S., Chometon, G., Eckes, B., Krieg, T., Lapière, C. M., Nusgens, B. V., Aumailley, M.
Formato: Texto
Lenguaje:English
Publicado: Birkhäuser-Verlag 2005
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2792356/
https://www.ncbi.nlm.nih.gov/pubmed/16378244
http://dx.doi.org/10.1007/s00018-005-5416-5
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author Zhang, Z.-G.
Lambert, C. A.
Servotte, S.
Chometon, G.
Eckes, B.
Krieg, T.
Lapière, C. M.
Nusgens, B. V.
Aumailley, M.
author_facet Zhang, Z.-G.
Lambert, C. A.
Servotte, S.
Chometon, G.
Eckes, B.
Krieg, T.
Lapière, C. M.
Nusgens, B. V.
Aumailley, M.
author_sort Zhang, Z.-G.
collection PubMed
description The GTP-binding proteins RhoA, Cdc42 and Rac1 regulate the organization and turnover of the cytoskeleton and cell-matrix adhesions, structures bridging cells to their support, and translating forces, external or generated within the cell. To investigate the specific requirements of Rho GTPases for biomechanical activities of clonal cell populations, we compared side-by-side stable lines of human fibroblasts expressing constitutively active (CA) RhoA, Cdc42 or Rac1. There was no marked effect of any CA GTPase on cell adhesion to different extracellular matrix proteins. Cell spreading was CA Rho GTPase specific and independent of the extracellular matrix proteins allowing adhesion. Mechanical properties were dramatically restricted by CA RhoA on bi- and in tri-dimensional surroundings, were boosted by CA Rac1 on bi-dimensional surroundings only, and were not or marginally affected by CA Cdc42. In conclusion, the action of Rho GTPases appears to depend on the task cells are performing.
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spelling pubmed-27923562009-12-23 Effects of constitutively active GTPases on fibroblast behavior Zhang, Z.-G. Lambert, C. A. Servotte, S. Chometon, G. Eckes, B. Krieg, T. Lapière, C. M. Nusgens, B. V. Aumailley, M. Cell Mol Life Sci Research Article The GTP-binding proteins RhoA, Cdc42 and Rac1 regulate the organization and turnover of the cytoskeleton and cell-matrix adhesions, structures bridging cells to their support, and translating forces, external or generated within the cell. To investigate the specific requirements of Rho GTPases for biomechanical activities of clonal cell populations, we compared side-by-side stable lines of human fibroblasts expressing constitutively active (CA) RhoA, Cdc42 or Rac1. There was no marked effect of any CA GTPase on cell adhesion to different extracellular matrix proteins. Cell spreading was CA Rho GTPase specific and independent of the extracellular matrix proteins allowing adhesion. Mechanical properties were dramatically restricted by CA RhoA on bi- and in tri-dimensional surroundings, were boosted by CA Rac1 on bi-dimensional surroundings only, and were not or marginally affected by CA Cdc42. In conclusion, the action of Rho GTPases appears to depend on the task cells are performing. Birkhäuser-Verlag 2005-12-27 2006-01 /pmc/articles/PMC2792356/ /pubmed/16378244 http://dx.doi.org/10.1007/s00018-005-5416-5 Text en © Birkhäuser Verlag, Basel 2006
spellingShingle Research Article
Zhang, Z.-G.
Lambert, C. A.
Servotte, S.
Chometon, G.
Eckes, B.
Krieg, T.
Lapière, C. M.
Nusgens, B. V.
Aumailley, M.
Effects of constitutively active GTPases on fibroblast behavior
title Effects of constitutively active GTPases on fibroblast behavior
title_full Effects of constitutively active GTPases on fibroblast behavior
title_fullStr Effects of constitutively active GTPases on fibroblast behavior
title_full_unstemmed Effects of constitutively active GTPases on fibroblast behavior
title_short Effects of constitutively active GTPases on fibroblast behavior
title_sort effects of constitutively active gtpases on fibroblast behavior
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2792356/
https://www.ncbi.nlm.nih.gov/pubmed/16378244
http://dx.doi.org/10.1007/s00018-005-5416-5
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