Rapid and Simple RPHPLC Method for the Estimation of Metformin in Rat Plasma

A simple reverse phase high-performance liquid chromatographic method has been developed for determining the concentration of metformin in rat plasma. The method employs C(18) column (300 mm × 2.4 mm i.d.), ammonium acetate (0.15 M) and acetonitrile (90:10; pH-5.5; 1.0 ml/min) as mobile phase and ul...

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Autores principales: Wanjari, M. M., There, A. W., Tajne, M. R., Chopde, C. T., Umathe, S. N.
Formato: Texto
Lenguaje:English
Publicado: Medknow Publications 2008
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2792473/
https://www.ncbi.nlm.nih.gov/pubmed/20046712
http://dx.doi.org/10.4103/0250-474X.41455
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author Wanjari, M. M.
There, A. W.
Tajne, M. R.
Chopde, C. T.
Umathe, S. N.
author_facet Wanjari, M. M.
There, A. W.
Tajne, M. R.
Chopde, C. T.
Umathe, S. N.
author_sort Wanjari, M. M.
collection PubMed
description A simple reverse phase high-performance liquid chromatographic method has been developed for determining the concentration of metformin in rat plasma. The method employs C(18) column (300 mm × 2.4 mm i.d.), ammonium acetate (0.15 M) and acetonitrile (90:10; pH-5.5; 1.0 ml/min) as mobile phase and ultraviolet detection at 236 nm. Acetonitrile was used to simultaneously deproteinize rat plasma and extract metformin. The assay was linear in the concentration range of 0.33 μg-16.6 μg/ml with co-efficient of correlation 0.994. The retention time was 4.7 min. The method was found to be precise (% CV < 15%), accurate and suitable for pharmacokinetic study of orally administered metformin in rats.
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spelling pubmed-27924732009-12-14 Rapid and Simple RPHPLC Method for the Estimation of Metformin in Rat Plasma Wanjari, M. M. There, A. W. Tajne, M. R. Chopde, C. T. Umathe, S. N. Indian J Pharm Sci Research Paper A simple reverse phase high-performance liquid chromatographic method has been developed for determining the concentration of metformin in rat plasma. The method employs C(18) column (300 mm × 2.4 mm i.d.), ammonium acetate (0.15 M) and acetonitrile (90:10; pH-5.5; 1.0 ml/min) as mobile phase and ultraviolet detection at 236 nm. Acetonitrile was used to simultaneously deproteinize rat plasma and extract metformin. The assay was linear in the concentration range of 0.33 μg-16.6 μg/ml with co-efficient of correlation 0.994. The retention time was 4.7 min. The method was found to be precise (% CV < 15%), accurate and suitable for pharmacokinetic study of orally administered metformin in rats. Medknow Publications 2008 /pmc/articles/PMC2792473/ /pubmed/20046712 http://dx.doi.org/10.4103/0250-474X.41455 Text en © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Paper
Wanjari, M. M.
There, A. W.
Tajne, M. R.
Chopde, C. T.
Umathe, S. N.
Rapid and Simple RPHPLC Method for the Estimation of Metformin in Rat Plasma
title Rapid and Simple RPHPLC Method for the Estimation of Metformin in Rat Plasma
title_full Rapid and Simple RPHPLC Method for the Estimation of Metformin in Rat Plasma
title_fullStr Rapid and Simple RPHPLC Method for the Estimation of Metformin in Rat Plasma
title_full_unstemmed Rapid and Simple RPHPLC Method for the Estimation of Metformin in Rat Plasma
title_short Rapid and Simple RPHPLC Method for the Estimation of Metformin in Rat Plasma
title_sort rapid and simple rphplc method for the estimation of metformin in rat plasma
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2792473/
https://www.ncbi.nlm.nih.gov/pubmed/20046712
http://dx.doi.org/10.4103/0250-474X.41455
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