Cargando…
Simultaneous Determination of Valsartan and Hydrochlorothiazide in Tablets by RP-HPLC
A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of valsartan and hydrochlorothiazide in tablets. A column having 200 × 4.6 mm i.d. in isocratic mode with mobile phase containing methanol:acetonitrile:water...
Autores principales: | , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications
2008
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2792532/ https://www.ncbi.nlm.nih.gov/pubmed/20046750 http://dx.doi.org/10.4103/0250-474X.43006 |
_version_ | 1782175251414646784 |
---|---|
author | Tian, D. F. Tian, X. L. Tian, T. Wang, Z. Y. Mo, F. K. |
author_facet | Tian, D. F. Tian, X. L. Tian, T. Wang, Z. Y. Mo, F. K. |
author_sort | Tian, D. F. |
collection | PubMed |
description | A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of valsartan and hydrochlorothiazide in tablets. A column having 200 × 4.6 mm i.d. in isocratic mode with mobile phase containing methanol:acetonitrile:water:isopropylalcohol (22:18:68:2; adjusted to pH 8.0 using triethylamine; v/v) was used. The flow rate was 1.0 ml/min and effluent was monitored at 270 nm. The retention time (min) and linearity range (μg/ml) for valsartan and hydrochlorothiazide were (3.42, 8.43) and (5-150, 78-234), respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of valsartan and hydrochlorothiazide in tablets. |
format | Text |
id | pubmed-2792532 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Medknow Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-27925322009-12-14 Simultaneous Determination of Valsartan and Hydrochlorothiazide in Tablets by RP-HPLC Tian, D. F. Tian, X. L. Tian, T. Wang, Z. Y. Mo, F. K. Indian J Pharm Sci Short Communications A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of valsartan and hydrochlorothiazide in tablets. A column having 200 × 4.6 mm i.d. in isocratic mode with mobile phase containing methanol:acetonitrile:water:isopropylalcohol (22:18:68:2; adjusted to pH 8.0 using triethylamine; v/v) was used. The flow rate was 1.0 ml/min and effluent was monitored at 270 nm. The retention time (min) and linearity range (μg/ml) for valsartan and hydrochlorothiazide were (3.42, 8.43) and (5-150, 78-234), respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of valsartan and hydrochlorothiazide in tablets. Medknow Publications 2008 /pmc/articles/PMC2792532/ /pubmed/20046750 http://dx.doi.org/10.4103/0250-474X.43006 Text en © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communications Tian, D. F. Tian, X. L. Tian, T. Wang, Z. Y. Mo, F. K. Simultaneous Determination of Valsartan and Hydrochlorothiazide in Tablets by RP-HPLC |
title | Simultaneous Determination of Valsartan and Hydrochlorothiazide in Tablets by RP-HPLC |
title_full | Simultaneous Determination of Valsartan and Hydrochlorothiazide in Tablets by RP-HPLC |
title_fullStr | Simultaneous Determination of Valsartan and Hydrochlorothiazide in Tablets by RP-HPLC |
title_full_unstemmed | Simultaneous Determination of Valsartan and Hydrochlorothiazide in Tablets by RP-HPLC |
title_short | Simultaneous Determination of Valsartan and Hydrochlorothiazide in Tablets by RP-HPLC |
title_sort | simultaneous determination of valsartan and hydrochlorothiazide in tablets by rp-hplc |
topic | Short Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2792532/ https://www.ncbi.nlm.nih.gov/pubmed/20046750 http://dx.doi.org/10.4103/0250-474X.43006 |
work_keys_str_mv | AT tiandf simultaneousdeterminationofvalsartanandhydrochlorothiazideintabletsbyrphplc AT tianxl simultaneousdeterminationofvalsartanandhydrochlorothiazideintabletsbyrphplc AT tiant simultaneousdeterminationofvalsartanandhydrochlorothiazideintabletsbyrphplc AT wangzy simultaneousdeterminationofvalsartanandhydrochlorothiazideintabletsbyrphplc AT mofk simultaneousdeterminationofvalsartanandhydrochlorothiazideintabletsbyrphplc |