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RP-HPLC Estimation of Risperidone in Tablet Dosage Forms
A simple, specific, accurate, and precise reverse phase liquid chromatographic method was developed and validated for the estimation of risperidone in tablet dosage forms. A Phenomenex Gemini C-18, 5 μm column having 250×4.6 mm i.d. in isocratic mode, with mobile phase containing methanol: acetonitr...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications
2008
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2792550/ https://www.ncbi.nlm.nih.gov/pubmed/20046778 http://dx.doi.org/10.4103/0250-474X.44601 |
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author | Baldania, S. L. Bhatt, K. K. Mehta, R. S. Shah, D. A. |
author_facet | Baldania, S. L. Bhatt, K. K. Mehta, R. S. Shah, D. A. |
author_sort | Baldania, S. L. |
collection | PubMed |
description | A simple, specific, accurate, and precise reverse phase liquid chromatographic method was developed and validated for the estimation of risperidone in tablet dosage forms. A Phenomenex Gemini C-18, 5 μm column having 250×4.6 mm i.d. in isocratic mode, with mobile phase containing methanol: acetonitrile: 50 mM potassium dihydrogen orthophosphate (80:10:10 v/v) was used. The flow rate was 1.3 ml/min and effluents were monitored at 234 nm. Clozapine was used as an internal standard. The retention time of risperidone and clozapine were 2.5 min and 3.3 min, respectively. The method was validated for linearity, accuracy, precision, specificity, limit of quantification, limit of detection, robustness and stability. The limit of detection and limit of quantification for estimation of risperidone was found to be 500 ng/ml and 990 ng/ml, respectively. Recovery of risperidone was found to be in the range of 99.02-101.68%. Proposed method was successfully applied for the quantitative determination of risperidone in tablet formulations. |
format | Text |
id | pubmed-2792550 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2008 |
publisher | Medknow Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-27925502009-12-14 RP-HPLC Estimation of Risperidone in Tablet Dosage Forms Baldania, S. L. Bhatt, K. K. Mehta, R. S. Shah, D. A. Indian J Pharm Sci Short Communications A simple, specific, accurate, and precise reverse phase liquid chromatographic method was developed and validated for the estimation of risperidone in tablet dosage forms. A Phenomenex Gemini C-18, 5 μm column having 250×4.6 mm i.d. in isocratic mode, with mobile phase containing methanol: acetonitrile: 50 mM potassium dihydrogen orthophosphate (80:10:10 v/v) was used. The flow rate was 1.3 ml/min and effluents were monitored at 234 nm. Clozapine was used as an internal standard. The retention time of risperidone and clozapine were 2.5 min and 3.3 min, respectively. The method was validated for linearity, accuracy, precision, specificity, limit of quantification, limit of detection, robustness and stability. The limit of detection and limit of quantification for estimation of risperidone was found to be 500 ng/ml and 990 ng/ml, respectively. Recovery of risperidone was found to be in the range of 99.02-101.68%. Proposed method was successfully applied for the quantitative determination of risperidone in tablet formulations. Medknow Publications 2008 /pmc/articles/PMC2792550/ /pubmed/20046778 http://dx.doi.org/10.4103/0250-474X.44601 Text en © Indian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communications Baldania, S. L. Bhatt, K. K. Mehta, R. S. Shah, D. A. RP-HPLC Estimation of Risperidone in Tablet Dosage Forms |
title | RP-HPLC Estimation of Risperidone in Tablet Dosage Forms |
title_full | RP-HPLC Estimation of Risperidone in Tablet Dosage Forms |
title_fullStr | RP-HPLC Estimation of Risperidone in Tablet Dosage Forms |
title_full_unstemmed | RP-HPLC Estimation of Risperidone in Tablet Dosage Forms |
title_short | RP-HPLC Estimation of Risperidone in Tablet Dosage Forms |
title_sort | rp-hplc estimation of risperidone in tablet dosage forms |
topic | Short Communications |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2792550/ https://www.ncbi.nlm.nih.gov/pubmed/20046778 http://dx.doi.org/10.4103/0250-474X.44601 |
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