Sample purification procedure for confirmation of 3′-hydroxy stanozolol by gas chromatography/high resolution mass spectrometry

OBJECTIVE: To improve the detection limit of 3′-hydroxy-stanozolol by using the double extraction procedure, specific for basic drugs. MATERIALS AND METHODS: The urine samples were spiked in four replicates with 3′-hydroxy-stanozolol at different concentrations of 1, 2, 5 and 10 ng/mL, processed by two different methods and injected into gas chromatography/high resolution mass spectrometry (GC-HRMS) instrument. The data was analyzed by comparing the recovery values and the ion match criterion of the two procedures. RESULTS: The results show that the recovery percentage and ion match criterion of 3′-hydroxy-stanozolol at lower concentrations has a significant improvement when Solid phase extraction was performed, instead of Liquid-liquid extraction in the second extraction procedure. CONCLUSION: The sample preparation procedure using Oasis-MCX cartridges allows confirmation of 3′-hydroxy-stanozolol at the minimum required performance limit (MRPL) decided by the World Anti Doping Agency. This procedure may be used for the confirmation of suspicious samples found in routine testing, as it efficiently fulfills the ion-matching criterion laid down by the World Anti Doping Agency.