Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus

We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay prod...

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Detalles Bibliográficos
Autores principales: Jayawardena, Shanthi, Cheung, Chung Y., Barr, Ian, Chan, Kwok H., Chen, Honglin, Guan, Yi, Peiris, J.S. Malik, Poon, Leo L.M.
Formato: Texto
Lenguaje:English
Publicado: Centers for Disease Control and Prevention 2007
Materias:
Dispatch
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2792861/
https://www.ncbi.nlm.nih.gov/pubmed/17553231
http://dx.doi.org/10.3201/eid1306.061572
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author Jayawardena, Shanthi
Cheung, Chung Y.
Barr, Ian
Chan, Kwok H.
Chen, Honglin
Guan, Yi
Peiris, J.S. Malik
Poon, Leo L.M.
author_facet Jayawardena, Shanthi
Cheung, Chung Y.
Barr, Ian
Chan, Kwok H.
Chen, Honglin
Guan, Yi
Peiris, J.S. Malik
Poon, Leo L.M.
author_sort Jayawardena, Shanthi
collection PubMed
description We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.
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spelling pubmed-27928612009-12-15 Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus Jayawardena, Shanthi Cheung, Chung Y. Barr, Ian Chan, Kwok H. Chen, Honglin Guan, Yi Peiris, J.S. Malik Poon, Leo L.M. Emerg Infect Dis Dispatch We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes. Centers for Disease Control and Prevention 2007-06 /pmc/articles/PMC2792861/ /pubmed/17553231 http://dx.doi.org/10.3201/eid1306.061572 Text en https://creativecommons.org/licenses/by/4.0/This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited.
spellingShingle Dispatch
Jayawardena, Shanthi
Cheung, Chung Y.
Barr, Ian
Chan, Kwok H.
Chen, Honglin
Guan, Yi
Peiris, J.S. Malik
Poon, Leo L.M.
Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus
title Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus
title_full Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus
title_fullStr Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus
title_full_unstemmed Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus
title_short Loop-Mediated Isothermal Amplification for Influenza A (H5N1) Virus
title_sort loop-mediated isothermal amplification for influenza a (h5n1) virus
topic Dispatch
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2792861/
https://www.ncbi.nlm.nih.gov/pubmed/17553231
http://dx.doi.org/10.3201/eid1306.061572
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