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Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart
Cardiac ischemia-reperfusion experiments in the mouse are important in vivo models of human disease. Infarct size is a particularly important scientific readout as virtually all cardiocirculatory pathways are affected by it. Therefore, such measurements must be exact and valid. The histological anal...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
American Physiological Society
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2793132/ https://www.ncbi.nlm.nih.gov/pubmed/19820193 http://dx.doi.org/10.1152/ajpheart.00836.2009 |
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author | Bohl, Steffen Medway, Debra J. Schulz-Menger, Jeanette Schneider, Jurgen E. Neubauer, Stefan Lygate, Craig A. |
author_facet | Bohl, Steffen Medway, Debra J. Schulz-Menger, Jeanette Schneider, Jurgen E. Neubauer, Stefan Lygate, Craig A. |
author_sort | Bohl, Steffen |
collection | PubMed |
description | Cardiac ischemia-reperfusion experiments in the mouse are important in vivo models of human disease. Infarct size is a particularly important scientific readout as virtually all cardiocirculatory pathways are affected by it. Therefore, such measurements must be exact and valid. The histological analysis, however, remains technically challenging, and the resulting quality is often unsatisfactory. For this report we have scrutinized each step involved in standard double-staining histology. We have tested published approaches and challenged their practicality. As a result, we propose an improved and streamlined protocol, which consistently yields high-quality histology, thereby minimizing experimental noise and group sizes. |
format | Text |
id | pubmed-2793132 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | American Physiological Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-27931322010-12-01 Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart Bohl, Steffen Medway, Debra J. Schulz-Menger, Jeanette Schneider, Jurgen E. Neubauer, Stefan Lygate, Craig A. Am J Physiol Heart Circ Physiol Articles Cardiac ischemia-reperfusion experiments in the mouse are important in vivo models of human disease. Infarct size is a particularly important scientific readout as virtually all cardiocirculatory pathways are affected by it. Therefore, such measurements must be exact and valid. The histological analysis, however, remains technically challenging, and the resulting quality is often unsatisfactory. For this report we have scrutinized each step involved in standard double-staining histology. We have tested published approaches and challenged their practicality. As a result, we propose an improved and streamlined protocol, which consistently yields high-quality histology, thereby minimizing experimental noise and group sizes. American Physiological Society 2009-12 2009-10-09 /pmc/articles/PMC2793132/ /pubmed/19820193 http://dx.doi.org/10.1152/ajpheart.00836.2009 Text en Copyright © 2009 the American Physiological Society This document may be redistributed and reused, subject to www.the-aps.org/publications/journals/funding_addendum_policy.htm (http://www.the-aps.org/publications/journals/funding_addendum_policy.htm) . |
spellingShingle | Articles Bohl, Steffen Medway, Debra J. Schulz-Menger, Jeanette Schneider, Jurgen E. Neubauer, Stefan Lygate, Craig A. Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart |
title | Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart |
title_full | Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart |
title_fullStr | Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart |
title_full_unstemmed | Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart |
title_short | Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart |
title_sort | refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2793132/ https://www.ncbi.nlm.nih.gov/pubmed/19820193 http://dx.doi.org/10.1152/ajpheart.00836.2009 |
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