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Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart

Cardiac ischemia-reperfusion experiments in the mouse are important in vivo models of human disease. Infarct size is a particularly important scientific readout as virtually all cardiocirculatory pathways are affected by it. Therefore, such measurements must be exact and valid. The histological anal...

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Detalles Bibliográficos
Autores principales: Bohl, Steffen, Medway, Debra J., Schulz-Menger, Jeanette, Schneider, Jurgen E., Neubauer, Stefan, Lygate, Craig A.
Formato: Texto
Lenguaje:English
Publicado: American Physiological Society 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2793132/
https://www.ncbi.nlm.nih.gov/pubmed/19820193
http://dx.doi.org/10.1152/ajpheart.00836.2009
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author Bohl, Steffen
Medway, Debra J.
Schulz-Menger, Jeanette
Schneider, Jurgen E.
Neubauer, Stefan
Lygate, Craig A.
author_facet Bohl, Steffen
Medway, Debra J.
Schulz-Menger, Jeanette
Schneider, Jurgen E.
Neubauer, Stefan
Lygate, Craig A.
author_sort Bohl, Steffen
collection PubMed
description Cardiac ischemia-reperfusion experiments in the mouse are important in vivo models of human disease. Infarct size is a particularly important scientific readout as virtually all cardiocirculatory pathways are affected by it. Therefore, such measurements must be exact and valid. The histological analysis, however, remains technically challenging, and the resulting quality is often unsatisfactory. For this report we have scrutinized each step involved in standard double-staining histology. We have tested published approaches and challenged their practicality. As a result, we propose an improved and streamlined protocol, which consistently yields high-quality histology, thereby minimizing experimental noise and group sizes.
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spelling pubmed-27931322010-12-01 Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart Bohl, Steffen Medway, Debra J. Schulz-Menger, Jeanette Schneider, Jurgen E. Neubauer, Stefan Lygate, Craig A. Am J Physiol Heart Circ Physiol Articles Cardiac ischemia-reperfusion experiments in the mouse are important in vivo models of human disease. Infarct size is a particularly important scientific readout as virtually all cardiocirculatory pathways are affected by it. Therefore, such measurements must be exact and valid. The histological analysis, however, remains technically challenging, and the resulting quality is often unsatisfactory. For this report we have scrutinized each step involved in standard double-staining histology. We have tested published approaches and challenged their practicality. As a result, we propose an improved and streamlined protocol, which consistently yields high-quality histology, thereby minimizing experimental noise and group sizes. American Physiological Society 2009-12 2009-10-09 /pmc/articles/PMC2793132/ /pubmed/19820193 http://dx.doi.org/10.1152/ajpheart.00836.2009 Text en Copyright © 2009 the American Physiological Society This document may be redistributed and reused, subject to www.the-aps.org/publications/journals/funding_addendum_policy.htm (http://www.the-aps.org/publications/journals/funding_addendum_policy.htm) .
spellingShingle Articles
Bohl, Steffen
Medway, Debra J.
Schulz-Menger, Jeanette
Schneider, Jurgen E.
Neubauer, Stefan
Lygate, Craig A.
Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart
title Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart
title_full Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart
title_fullStr Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart
title_full_unstemmed Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart
title_short Refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart
title_sort refined approach for quantification of in vivo ischemia-reperfusion injury in the mouse heart
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2793132/
https://www.ncbi.nlm.nih.gov/pubmed/19820193
http://dx.doi.org/10.1152/ajpheart.00836.2009
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