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Differential Effects of Thiazolidinediones on Adipocyte Growth and Recruitment in Zucker Fatty Rats
BACKGROUND: Adipose tissue grows by two mechanisms: hyperplasia (cell number increase) and hypertrophy (cell size increase). Thiazolidinediones are insulin-sensitizing peroxisome proliferator-activated receptor gamma agonists that are known to affect the morphology of adipose tissue. METHODOLOGY: In...
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2794370/ https://www.ncbi.nlm.nih.gov/pubmed/20041138 http://dx.doi.org/10.1371/journal.pone.0008196 |
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author | MacKellar, Jennifer Cushman, Samuel W. Periwal, Vipul |
author_facet | MacKellar, Jennifer Cushman, Samuel W. Periwal, Vipul |
author_sort | MacKellar, Jennifer |
collection | PubMed |
description | BACKGROUND: Adipose tissue grows by two mechanisms: hyperplasia (cell number increase) and hypertrophy (cell size increase). Thiazolidinediones are insulin-sensitizing peroxisome proliferator-activated receptor gamma agonists that are known to affect the morphology of adipose tissue. METHODOLOGY: In this study, adipose cell-size probability distributions were measured in six Zucker fa/fa rats over a period of 24 days, from four weeks of age, using micro-biopsies to obtain subcutaneous (inguinal) fat tissue from the animals. Three of the rats were gavaged daily with rosiglitazone, a thiazolidinedione, and three served as controls. These longitudinal probability distributions were analyzed to obtain the rate of increase in cell-size diameter in rosiglitazone-treated animals, and the hyperplasia induced by treatment quantitatively. CONCLUSIONS: We found that treatment leads to hypertrophy that leads to an approximately linear rate of cell diameter increase (2 [Image: see text]m/day), and that the hyperplasia evident in treated animals occurs largely within the first eight days of treatment. The availability of additional lipid storage due to treatment may alleviate lipotoxicity and thereby promote insulin sensitivity. The hypothesis that a TZD regimen involving repeated treatments of limited duration may suffice for improvements in insulin sensitivity merits further investigation. |
format | Text |
id | pubmed-2794370 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-27943702009-12-30 Differential Effects of Thiazolidinediones on Adipocyte Growth and Recruitment in Zucker Fatty Rats MacKellar, Jennifer Cushman, Samuel W. Periwal, Vipul PLoS One Research Article BACKGROUND: Adipose tissue grows by two mechanisms: hyperplasia (cell number increase) and hypertrophy (cell size increase). Thiazolidinediones are insulin-sensitizing peroxisome proliferator-activated receptor gamma agonists that are known to affect the morphology of adipose tissue. METHODOLOGY: In this study, adipose cell-size probability distributions were measured in six Zucker fa/fa rats over a period of 24 days, from four weeks of age, using micro-biopsies to obtain subcutaneous (inguinal) fat tissue from the animals. Three of the rats were gavaged daily with rosiglitazone, a thiazolidinedione, and three served as controls. These longitudinal probability distributions were analyzed to obtain the rate of increase in cell-size diameter in rosiglitazone-treated animals, and the hyperplasia induced by treatment quantitatively. CONCLUSIONS: We found that treatment leads to hypertrophy that leads to an approximately linear rate of cell diameter increase (2 [Image: see text]m/day), and that the hyperplasia evident in treated animals occurs largely within the first eight days of treatment. The availability of additional lipid storage due to treatment may alleviate lipotoxicity and thereby promote insulin sensitivity. The hypothesis that a TZD regimen involving repeated treatments of limited duration may suffice for improvements in insulin sensitivity merits further investigation. Public Library of Science 2009-12-24 /pmc/articles/PMC2794370/ /pubmed/20041138 http://dx.doi.org/10.1371/journal.pone.0008196 Text en This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. |
spellingShingle | Research Article MacKellar, Jennifer Cushman, Samuel W. Periwal, Vipul Differential Effects of Thiazolidinediones on Adipocyte Growth and Recruitment in Zucker Fatty Rats |
title | Differential Effects of Thiazolidinediones on Adipocyte Growth and Recruitment in Zucker Fatty Rats |
title_full | Differential Effects of Thiazolidinediones on Adipocyte Growth and Recruitment in Zucker Fatty Rats |
title_fullStr | Differential Effects of Thiazolidinediones on Adipocyte Growth and Recruitment in Zucker Fatty Rats |
title_full_unstemmed | Differential Effects of Thiazolidinediones on Adipocyte Growth and Recruitment in Zucker Fatty Rats |
title_short | Differential Effects of Thiazolidinediones on Adipocyte Growth and Recruitment in Zucker Fatty Rats |
title_sort | differential effects of thiazolidinediones on adipocyte growth and recruitment in zucker fatty rats |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2794370/ https://www.ncbi.nlm.nih.gov/pubmed/20041138 http://dx.doi.org/10.1371/journal.pone.0008196 |
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