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Toward Engineering Synthetic Microbial Metabolism
The generation of well-characterized parts and the formulation of biological design principles in synthetic biology are laying the foundation for more complex and advanced microbial metabolic engineering. Improvements in de novo DNA synthesis and codon-optimization alone are already contributing to...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796345/ https://www.ncbi.nlm.nih.gov/pubmed/20037734 http://dx.doi.org/10.1155/2010/459760 |
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author | McArthur, George H. Fong, Stephen S. |
author_facet | McArthur, George H. Fong, Stephen S. |
author_sort | McArthur, George H. |
collection | PubMed |
description | The generation of well-characterized parts and the formulation of biological design principles in synthetic biology are laying the foundation for more complex and advanced microbial metabolic engineering. Improvements in de novo DNA synthesis and codon-optimization alone are already contributing to the manufacturing of pathway enzymes with improved or novel function. Further development of analytical and computer-aided design tools should accelerate the forward engineering of precisely regulated synthetic pathways by providing a standard framework for the predictable design of biological systems from well-characterized parts. In this review we discuss the current state of synthetic biology within a four-stage framework (design, modeling, synthesis, analysis) and highlight areas requiring further advancement to facilitate true engineering of synthetic microbial metabolism. |
format | Text |
id | pubmed-2796345 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-27963452009-12-23 Toward Engineering Synthetic Microbial Metabolism McArthur, George H. Fong, Stephen S. J Biomed Biotechnol Review Article The generation of well-characterized parts and the formulation of biological design principles in synthetic biology are laying the foundation for more complex and advanced microbial metabolic engineering. Improvements in de novo DNA synthesis and codon-optimization alone are already contributing to the manufacturing of pathway enzymes with improved or novel function. Further development of analytical and computer-aided design tools should accelerate the forward engineering of precisely regulated synthetic pathways by providing a standard framework for the predictable design of biological systems from well-characterized parts. In this review we discuss the current state of synthetic biology within a four-stage framework (design, modeling, synthesis, analysis) and highlight areas requiring further advancement to facilitate true engineering of synthetic microbial metabolism. Hindawi Publishing Corporation 2010 2009-12-14 /pmc/articles/PMC2796345/ /pubmed/20037734 http://dx.doi.org/10.1155/2010/459760 Text en Copyright © 2010 G. H. McArthur IV and S. S. Fong. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Review Article McArthur, George H. Fong, Stephen S. Toward Engineering Synthetic Microbial Metabolism |
title | Toward Engineering Synthetic Microbial Metabolism |
title_full | Toward Engineering Synthetic Microbial Metabolism |
title_fullStr | Toward Engineering Synthetic Microbial Metabolism |
title_full_unstemmed | Toward Engineering Synthetic Microbial Metabolism |
title_short | Toward Engineering Synthetic Microbial Metabolism |
title_sort | toward engineering synthetic microbial metabolism |
topic | Review Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796345/ https://www.ncbi.nlm.nih.gov/pubmed/20037734 http://dx.doi.org/10.1155/2010/459760 |
work_keys_str_mv | AT mcarthurgeorgeh towardengineeringsyntheticmicrobialmetabolism AT fongstephens towardengineeringsyntheticmicrobialmetabolism |