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A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture
Background. Bone engineering requires thicker three-dimensional constructs than the maximum thickness supported by standard cell-culture techniques (2 mm). A flow-perfusion bioreactor was developed to provide chemotransportation to thick (6 mm) scaffolds. Methods. Polyurethane scaffolds, seeded with...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2009
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796393/ https://www.ncbi.nlm.nih.gov/pubmed/20037739 http://dx.doi.org/10.1155/2009/873816 |
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author | Sailon, Alexander M. Allori, Alexander C. Davidson, Edward H. Reformat, Derek D. Allen, Robert J. Warren, Stephen M. |
author_facet | Sailon, Alexander M. Allori, Alexander C. Davidson, Edward H. Reformat, Derek D. Allen, Robert J. Warren, Stephen M. |
author_sort | Sailon, Alexander M. |
collection | PubMed |
description | Background. Bone engineering requires thicker three-dimensional constructs than the maximum thickness supported by standard cell-culture techniques (2 mm). A flow-perfusion bioreactor was developed to provide chemotransportation to thick (6 mm) scaffolds. Methods. Polyurethane scaffolds, seeded with murine preosteoblasts, were loaded into a novel bioreactor. Control scaffolds remained in static culture. Samples were harvested at days 2, 4, 6, and 8 and analyzed for cellular distribution, viability, metabolic activity, and density at the periphery and core. Results. By day 8, static scaffolds had a periphery cell density of 67% ± 5.0%, while in the core it was 0.3% ± 0.3%. Flow-perfused scaffolds demonstrated peripheral cell density of 94% ± 8.3% and core density of 76% ± 3.1% at day 8. Conclusions. Flow perfusion provides chemotransportation to thick scaffolds. This system may permit high throughput study of 3D tissues in vitro and enable prefabrication of biological constructs large enough to solve clinical problems. |
format | Text |
id | pubmed-2796393 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-27963932009-12-23 A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture Sailon, Alexander M. Allori, Alexander C. Davidson, Edward H. Reformat, Derek D. Allen, Robert J. Warren, Stephen M. J Biomed Biotechnol Research Article Background. Bone engineering requires thicker three-dimensional constructs than the maximum thickness supported by standard cell-culture techniques (2 mm). A flow-perfusion bioreactor was developed to provide chemotransportation to thick (6 mm) scaffolds. Methods. Polyurethane scaffolds, seeded with murine preosteoblasts, were loaded into a novel bioreactor. Control scaffolds remained in static culture. Samples were harvested at days 2, 4, 6, and 8 and analyzed for cellular distribution, viability, metabolic activity, and density at the periphery and core. Results. By day 8, static scaffolds had a periphery cell density of 67% ± 5.0%, while in the core it was 0.3% ± 0.3%. Flow-perfused scaffolds demonstrated peripheral cell density of 94% ± 8.3% and core density of 76% ± 3.1% at day 8. Conclusions. Flow perfusion provides chemotransportation to thick scaffolds. This system may permit high throughput study of 3D tissues in vitro and enable prefabrication of biological constructs large enough to solve clinical problems. Hindawi Publishing Corporation 2009 2009-12-09 /pmc/articles/PMC2796393/ /pubmed/20037739 http://dx.doi.org/10.1155/2009/873816 Text en Copyright © 2009 Alexander M. Sailon et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Sailon, Alexander M. Allori, Alexander C. Davidson, Edward H. Reformat, Derek D. Allen, Robert J. Warren, Stephen M. A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture |
title | A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture |
title_full | A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture |
title_fullStr | A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture |
title_full_unstemmed | A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture |
title_short | A Novel Flow-Perfusion Bioreactor Supports 3D Dynamic Cell Culture |
title_sort | novel flow-perfusion bioreactor supports 3d dynamic cell culture |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796393/ https://www.ncbi.nlm.nih.gov/pubmed/20037739 http://dx.doi.org/10.1155/2009/873816 |
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